Cycloheximide (CHX; Sigma-Aldrich), anti-DYKDDDDK (Flag) G1 Affinity Resin (GenScript), phenylmethylsulfonyl fluoride (PMSF) (Gold Bio), immunoprecipitation (IP) lysis buffer (Thermo Scientific), TPCK-treated trypsin (Sigma-Aldrich), proteasome inhibitor MG132 (Carbobenzoxy-L-leucyl-L-leucyl-L-leucinal, Selleck chem), NH4Cl (Ammonium chloride, Selleck chem), and recombinant human IFN-α2 (GenScript) and IFN-γ (GenScript) were purchased from the indicated manufacturers. Antibodies against JAK1, STAT1, phospho-STAT1, and β-actin were purchased from Sigma-Aldrich; antibodies against SOCS1 and SOCS3 were purchased from GeneTex, antibodies against influenza virus NP, M1, and NS1 were purchased from GeneTex; antibodies against DYKDDDDK (Flag) tag and HA tag were purchased from Cell Signaling Technology. Human SOCS1 siRNAs si-1, GCAUCCGCGUGCACUUUCAdTdT, and si-2, CUACCUGAGCUCCUUCCCCdTdT were synthesized by Gene Pharma.
Antibodies against dykddddk flag tag and ha tag
Manufactured by Cell Signaling Technology
Antibodies against DYKDDDDK (Flag) tag and HA tag are laboratory tools used to detect and study proteins that have been tagged with these specific peptide sequences. These antibodies can be used in various applications, such as immunoprecipitation, Western blotting, and immunofluorescence, to identify and analyze the tagged proteins.
Automatically generated - may contain errors
Lab products found in correlation
Cycloheximide (chx), by Merck Group (2 mentions)
Antibodies against socs1 and socs3, by GeneTex (2 mentions)
Nh4cl, by Selleck Chemicals (2 mentions)
Tpck treated trypsin, by Merck Group (1 mentions)
Anti dykddddk flag g1 affinity resin, by GenScript (1 mentions)
Immunoprecipitation ip lysis buffer, by Thermo Fisher Scientific (1 mentions)
β actin, by Merck Group (1 mentions)
Ifn γ, by GenScript (1 mentions)
Mg132, by Selleck Chemicals (1 mentions)
Proteasome inhibitor mg132, by Selleck Chemicals (1 mentions)
2 protocols using antibodies against dykddddk flag tag and ha tag
1
Interferon Signaling Pathway Modulation
2
Investigating SOCS1 Signaling Pathways
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Cycloheximide (CHX; Sigma-Aldrich), anti-DYKDDDDK (FLAG) G1 A nity Resin (GenScript), phenylmethylsulfonyl uoride (PMSF) (Gold Bio), immunoprecipitation (IP) lysis buffer (Thermo Scienti c), proteasome inhibitor MG132 (Selleck chem), NH 4 Cl (Selleck chem), and recombinant human IFN-α2 and IFN-γ (GenScript) were purchased from the indicated manufacturers. Antibodies against JAK1, STAT1, phospho-STAT1, and β-actin were purchased from Sigma; antibodies against SOCS1 and SOCS3 were purchased from GeneTex, antibodies against in uenza virus NP, M1, and NS1 were purchased from GeneTex; antibodies against DYKDDDDK (FLAG) tag and HA tag were purchased from Cell Signaling Technology. Human SOCS1 siRNAs si-1, GCAUCCGCGUGCACUUUCAdTdT, and si-2, CUACCUGAGCUCCUUCCCCdTdT were synthesized by Gene Pharma.
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