F344/Stm (NBRP-Rat No. 0140) rats were provided by the National Bio Resource Project for the Rat in Japan (www.anim.med.kyoto-u.ac.jp/nbr ). Jcl:Wistar rats and C57BL/6JJcl mice were obtained from CLEA Japan Inc. (Tokyo, Japan). The animals were kept under conditions of 50% humidity and a 14:10 h light:dark cycle. The newly developed W-Rosaem1(CAG-GFP)Kyo rats (NBRP-Rat No. 0770) were deposited into the National Bio Resource Project—Rat in Japan. They were fed a standard pellet diet (F-2, Oriental Yeast Co., Tokyo, Japan) and tap water ad libitum. Animal care and experiments conformed to the Guidelines for Animal Experiments of Kyoto University and were approved by the Animal Research Committee of Kyoto University.
C57bl 6jjcl mice
Manufactured by CLEA Japan
Sourced in Japan
C57BL/6JJcl mice are a inbred mouse strain commonly used in biomedical research. They are a sub-strain of the C57BL/6J strain, which is one of the most widely used mouse models. C57BL/6JJcl mice are genetically homogeneous and exhibit consistent biological characteristics.
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Lab products found in correlation
Prism 4, by GraphPad (1 mentions)
B6 cg tg k18 ace2 2prlmn j k18 hace2 mice, by Jackson ImmunoResearch (1 mentions)
Ain93g fomula diet, by Oriental Yeast (1 mentions)
C57bl 6jjmsslc mice, by Japan SLC (1 mentions)
Syrian hamsters, by Japan SLC (1 mentions)
F344 jcl rats, by CLEA Japan (1 mentions)
3 hydroxybenzylhydrazine, by Merck Group (1 mentions)
Tropicamide, by Santen (1 mentions)
Phenylephrine hydrochloride, by Santen (1 mentions)
Crf 1, by Oriental Yeast (1 mentions)
B6 cg tg nes cre 1kln j line, by Jackson ImmunoResearch (1 mentions)
Male icr mice, by CLEA Japan (1 mentions)
Cb 17 icr jcl mice, by CLEA Japan (1 mentions)
C b 17 icr scid scidjcl mice, by CLEA Japan (1 mentions)
C57bl 6j mice, by Charles River Laboratories (1 mentions)
Metformin, by Fujifilm (1 mentions)
D12331, by Research Diets (1 mentions)
B6 cb17 prkdcscid szj scid mice, by Jackson ImmunoResearch (1 mentions)
Gfap cre mice, by Jackson ImmunoResearch (1 mentions)
37 protocols using c57bl 6jjcl mice
1
Rat and Mouse Husbandry Protocol
2
NASH Model Liver Effects Study
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Example 6
Effect on Livers in Non-Alcoholic Steatohepatitis (NASH) Models
This study is conducted using NASH model mice/STAM (registered trademark) model mice (Medical Molecular Morphology, 46, 141 (2013)) from Stelic Institute & Co., Inc.
Fourteen-day-pregnant C57BL6J/JcL mice (CLEA Japan, Inc.) are fed and allowed to give the birth. Two-day-old mice are subcutaneously injected with streptozotocin (SIGMA-ALDRICH JAPAN) in physiological saline (Japanese Pharmacopoeia, Otsuka Pharmaceutical Co., Ltd.) one time to their backs. After 4 weeks of age, the mice are fed with high fat diet (High Fat Diet 32 (sterilized by radiation, CLEA Japan, Inc.) until the end of the experimental.
The compound is orally administered daily from 5- or 6-week-old. At 9- or 11-week-old, the animals are sacrificed under anesthesia. The livers are collected and their wet weights are measured. Paraffin sections or frozen sections are prepared from part of the livers, and are histopathologically examined, and the NAFLD activity score is measured. Further, RNA is extracted from the part of the livers, and the expression of fibrosis marker gene is measured by a quantitative PCR method. The results are statistically analyzed using EXSUS or Prism 4 (manufactured by GraphPad Software).
3
Bdnf Mutant Mice Husbandry Protocol
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All animal procedures were conducted in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and approved by the Keio University Institutional Animal Care and Use Committee. Male C57BL/6JJcl mice were obtained from CLEA Japan, Inc. (Tokyo, Japan). Bdnf mutant mice were generated as described below. All mice received food and water ad libitum and were housed in an atmosphere-controlled room with 12-hour light/dark cycles.
4
Temperature-Modulated SARS-CoV-2 Infection in Mice
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Six-week-old female C57BL/6JJmsSlc mice and 4-week-old female Syrian hamsters obtained from Japan SLC, Inc. were used as WT controls. For some experiments we used aged (52- to 122-week-old) female C57BL/6JJcl mice obtained from CLEA Japan, Inc. (Fig. 1e, f and Supplementary Figs. 7 and 10 ). B6.Cg-Tg(K18-ACE2)2Prlmn/J (K18-hACE2) mice were purchased from The Jackson Laboratory and subsequently bred at The University of Tokyo. Mice were kept in an incubator (MIR-154, PHCbi, Japan) at 4 °C (80% relative humidity), 22 °C (53% relative humidity), or 36 °C (25% relative humidity). Cold, RT, or high-heat exposures were started 7 days before infection and continued for the entire duration of the experiments. These mice were allowed free access to food and drinking water and kept on a 12 h light/dark cycle. All animals used in this study were fed with a γ-ray-sterilized normal diet (CE-2, CLEA Japan, Inc.) or AIN93G-fomula diet (Oriental Yeast, Japan) as a LF diet. We confirmed that drinking water did not freeze at 4 °C. All animals were euthanized with deep anesthesia and cervical dislocation. All animal experiments were performed in accordance with The University of Tokyo’s Regulations for Animal Care and Use, which were approved by the Animal Experiment Committee of the Institute of Medical Science, The University of Tokyo (PA15-92, PA19-87, PA22-33).
5
Adenine-Induced Tubular Injury Model
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All animal experiments were approved by the Animal Committee of Tohoku University School of Medicine (2016PhA-019) on 8 February 2016. Experimental protocols and animal care were performed according to the guidelines for the care and use of animals established by Tohoku University. Male 8- to 9-week-old C57BL/6JJcl mice were purchased from CLEA Japan, Inc. (Tokyo, Japan). The mice were randomized to control and RF groups. Control-group mice were fed a normal diet (Oriental Yeast, Tokyo, Japan) for 7 weeks. RF-group mice were fed a diet containing 0.2% wt/wt adenine (Oriental Yeast) for 7 weeks to induce tubular injury [47 (link)], a model we used in previous work [17 (link)], with other mice. After 7 weeks, each group was further divided into two groups, one of which received 8% (wt/wt) AST-120 (Kremezin®; Kureha Pharmaceuticals, Tokyo, Japan). The concentration of AST-120 was selected based on findings from previous studies [25 (link),48 (link)]. After 4 weeks, all mice were euthanized, and blood and organ samples were collected. Perfusion with saline was performed before organ collection. Blood samples were collected into EDTA-treated tubes.
6
Mice Housing and Acclimation
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We purchased male C57BL/6JJcl mice (age: 27‐35 weeks) from CLEA Japan, Inc (Tokyo, Japan). All mice were housed in pairs (five to six per cage) in a temperature‐controlled animal holding room at 23 ± 1°C, with a relative humidity of 55 ± 10% under a 12‐hours/12‐hours light/dark cycle (lights on 8:00 am ‒ off 8:00 pm ). They were given ad libitum access to a commercial diet and tap water. All animals were considered healthy based on physical examination.
7
Parvalbumin-Expressing Neuron Characterization
8
C57BL/6JJcl Mice Nutrition Study
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Five-week-old male C57BL/6JJcl mice (n = 15) (CLEA Japan, Inc., Tokyo, Japan) were housed under specific pathogen free conditions at a temperature of 23 ± 2°C and a humidity of 60 ± 15% with a 12-h light-dark cycle, and were allowed free access to water and a special AIN-93G diet containing VD3 (200 IU/100 g).
9
Murine Skin Extraction for MG Suspension
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Small pieces of murine skin were harvested to prepare MG suspension. C57BL/6JJcl mice (male, 8–9 weeks old) (CLEA Japan, Inc., Osaka, Japan) were shaved with an electric shaver (Thrive; Daito Electric Machine Ind. Co., Ltd., Tokyo, Japan) and depilated using depilation cream (Kracie, Tokyo, Japan). The skin of the mice was obtained using an 8 mm punch (Kai Industries Co., Ltd., Tokyo, Japan) after euthanasia by carbon dioxide gas inhalation. Two skin sheets of 8 mm in diameter (total area: approximately 100 mm2) were cut into small pieces using scissors and placed into Rigeneracons with 1 mL saline solution (Otsuka Pharmaceutical Factory, Inc., Tokyo, Japan). The skin was minced using a rotating blade in Rigeneracons for 2 minutes and passed through the blade holes (50 μm in diameter) to generate the MG suspension.
10
High-Pressure Skin Graft Preservation
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Briefly, C57BL6J/Jcl mice (CLEA Japan Inc., Tokyo, Japan) were euthanized by carbon dioxide inhalation and their skin backs were resected. Then, 8-mm punch biopsy tools (Kai Industries Ltd., Gifu, Japan) were used to obtain full-thickness skin grafts (total of 80 skin grafts). Ten grafts were packed into each of 25-ml cryopreservation bottles (Perfluoroalkoxy [PFA] bottle; Tech-jam, Osaka, Japan) filled with DMEM solution (Nissui Pharmaceutical Co.). Skin grafts in each bottle were pressurized at 50 MPa for 36 h using a new automated HHP device as mentioned above or at 200 MPa for 10 min using another custom-made device (Echigo Seika Co.), as described in our previous studies11 (link),30 (link) (Fig. 1 b). After pressurization, those specimens were washed 2 times with PBS and transplanted to the dorsal fascia of other mice (2 grafts per mouse, total 12 grafts per group). We then covered the grafts with collagen sheets and closed the skin by nylon 5-0 sutures (Fig. 1 b).
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