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Dakoenvision system hrp labelled polymer anti rabbit secondary antibody

Manufactured by Agilent Technologies
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The DakoEnVision+ System-HRP Labelled Polymer Anti-Rabbit secondary antibody is a laboratory reagent used in immunohistochemistry (IHC) applications. It is designed to detect and amplify the signal from primary antibodies that have bound to target antigens in tissue samples. The antibody is conjugated with horseradish peroxidase (HRP) enzyme, which catalyzes a colorimetric reaction to visualize the antigen-antibody complexes.

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Lab products found in correlation

Las ez software, by Leica (2 mentions) Rabbit anti phospho erk thr202 tyr204 d13.14.4e, by Cell Signaling Technology (2 mentions) Dab chromogen, by Agilent Technologies (2 mentions) Dmlb microscope, by Leica (2 mentions)

Close spelling variants of this product

HRP-labelled secondary antibody Labelled polymer-HRP Anti-rabbit secondary antibody Polymer-HRP Anti-TM

2 protocols using dakoenvision system hrp labelled polymer anti rabbit secondary antibody

1

Phospho-ERK Immunohistochemistry in NOD/SCID Mice

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Formalin-fixed, paraffin-embedded tumor material from NOD (nonobese diabetic)/SCID (severe combined immunodeficient) mice (in compliance with all relevant ethical regulations) were used from a previous study50 (link), and subjected to immunoperoxidase staining with rabbit anti-phospho-ERK (Thr202/Tyr204)(D13.14.4E) (Cell Signaling #4370).
After incubation with DakoEnVision+ System-HRP Labelled Polymer Anti-Rabbit secondary antibody (Dako #K4002), immunoreactivity was revealed by incubation in DAB chromogen (Dako) and sections were counterstained with Hematoxylin. Images were captured with the Leica LAS EZ software using a Leica DM LB microscope. Morphometric quantitation was performed by ImageJ software using spectral image segmentation. Software outputs were manually verified by visual inspection of digital images.
Ponsioen B., Post J.B., des Amorie J.R., Laskaris D., van Ineveld R.L., Kersten S., Bertotti A., Sassi F., Sipieter F., Cappe B., Mertens S., Verlaan-Klink I., Boj S.F., Vries R.G., Rehmann H., Vandenabeele P., Riquet F.B., Trusolino L., Bos J.L, & Snippert H.J. (2021). Quantifying single-cell ERK dynamics in colorectal cancer organoids reveals EGFR as an amplifier of oncogenic MAPK pathway signaling. Nature cell biology, 23(4), 377-390.
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2

Phospho-ERK Immunohistochemistry in NOD/SCID Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Formalin-fixed, paraffin-embedded tumor material from NOD (nonobese diabetic)/SCID (severe combined immunodeficient) mice (in compliance with all relevant ethical regulations) were used from a previous study50 (link), and subjected to immunoperoxidase staining with rabbit anti-phospho-ERK (Thr202/Tyr204)(D13.14.4E) (Cell Signaling #4370).
After incubation with DakoEnVision+ System-HRP Labelled Polymer Anti-Rabbit secondary antibody (Dako #K4002), immunoreactivity was revealed by incubation in DAB chromogen (Dako) and sections were counterstained with Hematoxylin. Images were captured with the Leica LAS EZ software using a Leica DM LB microscope. Morphometric quantitation was performed by ImageJ software using spectral image segmentation. Software outputs were manually verified by visual inspection of digital images.
Ponsioen B., Post J.B., des Amorie J.R., Laskaris D., van Ineveld R.L., Kersten S., Bertotti A., Sassi F., Sipieter F., Cappe B., Mertens S., Verlaan-Klink I., Boj S.F., Vries R.G., Rehmann H., Vandenabeele P., Riquet F.B., Trusolino L., Bos J.L, & Snippert H.J. (2021). Quantifying single-cell ERK dynamics in colorectal cancer organoids reveals EGFR as an amplifier of oncogenic MAPK pathway signaling. Nature cell biology, 23(4), 377-390.
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