Bafilomycin a1

Manufactured by MedChemExpress

Sourced in United States, China

Bafilomycin A1 is a macrolide lactone compound isolated from the culture broth of Streptomyces species. It functions as a highly potent and selective inhibitor of vacuolar-type H+-ATPases (V-ATPases), which are proton pumps that regulate intracellular pH and membrane potential across various cell membranes.

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Lab products found in correlation

3 methyladenine, by MedChemExpress (5 mentions) Chloroquine, by Merck Group (4 mentions) Rapamycin, by MedChemExpress (4 mentions) Mg132, by Merck Group (4 mentions) Z vad fmk, by Selleck Chemicals (3 mentions) Mg132, by MedChemExpress (3 mentions) Mg132, by Selleck Chemicals (3 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions) Cycloheximide (chx), by Selleck Chemicals (2 mentions) Bortezomib, by MedChemExpress (2 mentions) Hydrocortisone, by MedChemExpress (2 mentions) Hydroxychloroquine, by MedChemExpress (2 mentions) Leupeptin hemisulfate, by MedChemExpress (2 mentions) Dextran sulfate sodium salt, by MedChemExpress (2 mentions) Nigericin sodium salt, by Selleck Chemicals (2 mentions) Curcumin, by MedChemExpress (2 mentions) Lipopolysaccharide lps from escherichia coli o111 b4, by Merck Group (2 mentions) Nh4cl, by Sangon (2 mentions) β mercaptoethanol, by Thermo Fisher Scientific (2 mentions) Disuccinimidyl suberate dss, by MedChemExpress (2 mentions)

Spelling variants of this product

Bafilomycin A1 (Baf A1) (18 citations) Bafilomycin A1 (HY-100558) (5 citations) Bafilomycin A1 (BAF), (2 citations)

44 protocols using bafilomycin a1

Induction and Assessment of Acute Kidney Injury in Mice

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Numbers were randomly assigned to each mouse and split equally into groups for each study. Another individual performed assays and data analyses and was blinded to group assignments until analyses were completed. Rhabdo-AKI was induced in 13- to 14-week-old male mice with intramuscular glycerol, unilateral IRI-AKI with delayed nephrectomy (IRI-AKI DN) was performed on 10- to 12-week-old male mice, and tGFR were measured at day 27; each procedure was as previously described (52 (link), 55 (link), 96 (link)). Mice that underwent right nephrectomy alone were controls. For bilateral IRI-AKI, 10- to 12-week-old male mice underwent bilateral renal pedicle clamping for 28 minutes. For bafilomycin A1 studies, mice were injected IP with 3 mg/kg bafilomycin A1 (Med Chem Express LLC, 50-196-7747) in corn oil 1 hour before IRI-AKI surgery, as described (27 (link)). Controls were given corn oil. Detailed information about AKI models and renal function assays is provided in Supplemental Methods.

Yang M., Lopez L.N., Brewer M., Delgado R., Menshikh A., Clouthier K., Zhu Y., Vanichapol T., Yang H., Harris R.C., Gewin L., Brooks C.R., Davidson A.J, & de Caestecker M. (2023). Inhibition of retinoic acid signaling in proximal tubular epithelial cells protects against acute kidney injury. JCI Insight, 8(20), e173144.

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Induction and Assessment of Acute Kidney Injury in Mice

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Hypoxia-Induced Cell Signaling Assay

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Human embryonic kidney cells (HEK293) and mouse aortic VSM cells (MOVAS) (ATCC) were seeded at 60–70% confluence in Dulbecco modified eagle medium (DMEM) (Hyclone), supplemented with 10% fetal bovine serum (Hyclone) and 1% penicillin/streptomycin (Hyclone). Transfections of WNK4, KLHL3, and JAB1 plasmid (preserved in our laboratory) and JAB1 siRNA (GenePharma) were carried out using Lipofectamine 2000 (Thermo Fisher) and OptiMEM (Thermo Fisher) following the manufacturer's instructions. After transfection for 24 h, cells were incubated at 5% CO₂ level with 1% O₂ for 16 h. Meanwhile, we used 100 nM bafilomycin A1 (MedChemExpress) in the DMEM and then collected cells for further Western blot and immunofluorescence (IF), respectively.

Zhang Y., Jiang G, & Zhang C. (2021). Downregulation of Cullin 3 Ligase Signaling Pathways Contributes to Hypertension in Preeclampsia. Frontiers in Cardiovascular Medicine, 8, 654254.

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Pharmacological Modulation of Cellular Dynamics

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Cells were treated with the following drugs with the given concentration and incubation time: Golgicide A 10 µM 30 min (Merck, catalog # G0923), Bafilomycin A1 100 nM 1 h (MedChemExpress, catalog # HY-100558), Histamine 100 µM cells immediately imaged (Merck, catalog # H7125), PF-573228 10 µM 15 min (Merck, catalog # PZ0117), Nocodazole 10 µM 1 h (Merck, catalog # M1404), Cytochalasin D 500 nM 1 h (Merck, catalog # C8273), Para-nitroblebbistatin 20 µM 15 min (Cayman Chemical Company, item 24171), β-methyl-cyclodextrin 5 mM 15 min (Merck, catalog # C4555). Hypo-osmotic shocks were made by adding water in the media with a volume ratio of 1:1 and cells were imaged 15 min after. For all drug conditions, a paired design has been used: the same cell is imaged before and after the treatment.

Lachuer H., Le L., Lévêque-Fort S., Goud B, & Schauer K. (2023). Spatial organization of lysosomal exocytosis relies on membrane tension gradients. Proceedings of the National Academy of Sciences of the United States of America, 120(8), e2207425120.

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Autophagy and Wnt Signaling Modulation

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Bafilomycin A1 (Autophagy inhibitor), XAV939 (β-catenin inhibitor) and SKL2001(β-catenin agonist) were from MedChemExpress.

Yan B., Luo J., Kaltenmeier C., Du Q., Stolz D.B., Loughran P., Yan Y., Cui X, & Geller D.A. (2020). Interferon Regulatory Factor-1 (IRF1) activates autophagy to promote liver ischemia/reperfusion injury by inhibiting β-catenin in mice. PLoS ONE, 15(11), e0239119.

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Investigating Chloroquine and NAC

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Chloroquine (CQ) and N-Acetylcysteine (NAC) were purchased from Sigma-Aldrich, Mirin and Bafilomycin A1 (Baf) were obtained from MedChemExpress, and Panobinostat (LBH) was provided by Novartis Pharmaceuticals.

Ovejero-Sánchez M., González-Sarmiento R, & Herrero A.B. (2021). Synergistic effect of Chloroquine and Panobinostat in ovarian cancer through induction of DNA damage and inhibition of DNA repair. Neoplasia (New York, N.Y.), 23(5), 515-528.

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Screening Receptor Tyrosine Kinase Inhibitors

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Ceritinib (HY-15656), NVP-TAE684 (HY-10192), ASP3026 (HY-13326), and bafilomycin A1 (HY-100558) were purchased from MedChemExpress, NJ, USA. GSK1904529A (S1093) was purchased from Selleckchem and FAK-inhibitor 14 (sc-203950) from Santa Cruz. RTK(i)s used for screening were purchased from MedChemExpress, NJ, USA and are listed in Table S1. The inhibitors drugs were prepared as 1–10 mM stock in DMSO according to the solubility. Poly (2-hydroxyethyl methacrylate) (PolyHEMA) (P3932-10G) was purchased from Sigma Aldrich, St. Louis, MO, USA. Corning^® Matrigel^® Basement Membrane Matrix, LDEV-free (354234) was purchased from Corning, Bedford, MA, USA.

Myint K.Z., Balasubramanian B., Venkatraman S., Phimsen S., Sripramote S., Jantra J., Choeiphuk C., Mingphruedhi S., Muangkaew P., Rungsakulkij N., Tangtawee P., Suragul W., Farquharson W.V., Wongprasert K., Chutipongtanate S., Sanvarinda P., Ponpuak M., Poungvarin N., Janvilisri T., Suthiphongchai T., Yacqub-Usman K., Grabowska A.M., Bates D.O, & Tohtong R. (2024). Therapeutic Implications of Ceritinib in Cholangiocarcinoma beyond ALK Expression and Mutation. Pharmaceuticals, 17(2), 197.

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Comprehensive Protocol for Cell Stress Assays

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JNUTS013 (≥98% purity) was synthesized in the Tang laboratory. Lipopolysaccharide (LPS, from Escherichia coli O111:B4) was purchased from Sigma-Aldrich (Cat#L2630, Steinheim, Germany). Dimethyl sulfoxide (DMSO) was purchased from Aladdin Bio-Chem Technology (Cat#67–68-5, Shanghai, China). d-galactosamine (D-galactosamine) was purchased from Sigma-Aldrich (Cat#G1639, Steinheim, Germany). Hydrocortisone (Cat#HY-N0583), curcumin (Cat#HY-N0005), rapamycin (Cat#HY-10219), MG132 (Cat#HY-13259), bortezomib (Cat#HY-10227), hydroxychloroquine (Cat#HY-W031727), leupeptin hemisulfate (Cat#HY-18234A), bafilomycin A1 (Cat#HY-100558), 3-methyladenine (Cat#HY-19312), dextran sulfate sodium salt (DSSS, Cat#HY-116282B) and disuccinimidyl suberate (DSS) (Cat#HY-W019543) were purchased from MedChemExpress (Monmouth Junction, NJ, USA). Phorbol 12-myristate 13-acetate (PMA, Cat#S7791), nigericin sodium salt (Cat#S6653), cycloheximide (Cat#S7418) and Z-VAD-FMK (Cat#S7023) were purchased from Selleckchem (Houston, TX, USA). NP-40 lysis buffer (Cat#K1127) was purchased from Apexbio (Houston, TX, USA). NH₄Cl (Cat#A100621) was purchased from Sangon Biotech (Shanghai, China). β-Mercaptoethanol was from Thermo/Fisher (Cat#BP176100, Waltham, MA, USA). Details of other materials and suppliers are provided in the specific sections.

Wang F., Zhang M., Yuan M., Xia Z., Yang F., Zhang S., Lin T., Luo L., Tang J, & Zhang Y. (2023). A novel sorbicillinoid compound as a potent anti-inflammation agent through inducing NLRP3 protein degradation. British journal of pharmacology, 180(15), 1930-1948.

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Investigating Autophagy and Signaling Pathways

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Antibodies against mTOR (7C10) rabbit mAb (catalog 2983), phospho-mTOR (Ser2448) antibody (catalog 2971), p70 S6K antibody (catalog 9202), phospho-p70 S6K (Thr389) antibody (catalog 9205), AMPKα (D5A2) rabbit mAb (catalog 5831), phospho-AMPKα (Thr172) (40H9) rabbit mAb (catalog 2535), Atg5 (D5F5U) rabbit mAb (catalog 12994), Beclin 1 (D40C5) rabbit mAb (catalog 3495), SQSTM1/p62 antibody (catalog 5114), Akt antibody (catalog 9272), phospho-Akt (Ser473) antibody (catalog 9271), p44/42 MAPK (Erk1/2) antibody (catalog 9102), phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) rabbit mAb (catalog 4377), and LC3A/B (D3U4C) XP rabbit mAb (catalog 12741) were obtained from Cell Signaling Technologies. Antibody against PARP (catalog CY6850) was obtained from Abways Technology. Antibody against β-actin (catalog A5441) was from MilliporeSigma. Sertraline (catalog S6319), fluphenazine (catalog PHR1792), erlotinib (catalog CDS022564), chloroquine phosphate (catalog PHR1258), avertin (catalog T48402, 152463), and dimethyl sulfoxide (catalog D2650) were purchased from MilliporeSigma. SCH772984 (catalog HY-50846), rapamycin (catalog HY-10219), dorsomorphin (catalog HY-13418A), and bafilomycin A1 (catalog HY-100558) were obtained from MedChemExpress, and 3-MA (catalog S2767) and Z-VAD-FMK (catalog S7023) were obtained from Selleck. All compounds were dissolved in dimethyl sulfoxide.

Jiang X., Lu W., Shen X., Wang Q., Lv J., Liu M., Cheng F., Zhao Z, & Pang X. (2018). Repurposing sertraline sensitizes non–small cell lung cancer cells to erlotinib by inducing autophagy. JCI Insight, 3(11), e98921.

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Pharmacology of Novel Compounds

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Erastin (Catalog # HY-15,763), Cytochalasin D (Catalog # HY-N6682), 2-Deoxy-D-glucose (Catalog # HY-13,966), Rosiglitazone (Catalog # HY-17,386), T0070907 (Catalog # HY-13,202), and Bafilomycin A1 (Catalog # HY-100,558) were purchased from MedChem Express (Monmouth Junction, NJ, USA). MDA (Catalog # ab243066) was purchased from Abcam (Cambridge, MA, USA), ROS (Catalog # S0033S) was purchased from Beyotime (Shanghai, China), Recombinant human STC-1 (Catalog # 9400-S0-050) was purchased from R&D (Boston, MO, USA). Clodronate liposome was purchased from Liposoma B.V (Vrije University, Netherlands). LPS derived from Escherichia coli (Catalog # 0111: B4) was purchased from Sigma-Aldrich (St. Louis, MO, USA).

Wang J., Li J., Yin L., Wang X., Dong Y., Zhao G., Shen S, & Hou Y. (2024). MSCs promote the efferocytosis of large peritoneal macrophages to eliminate ferroptotic monocytes/macrophages in the injured endometria. Stem Cell Research & Therapy, 15, 127.

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