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Mouse anti inos antibody

Manufactured by BD
Sourced in United States

The mouse anti-iNOS antibody is a reagent used for the detection and quantification of inducible nitric oxide synthase (iNOS) in biological samples. iNOS is an enzyme that catalyzes the production of nitric oxide, a signaling molecule involved in various physiological and pathological processes. The antibody can be used in techniques such as Western blotting, immunohistochemistry, and ELISA to identify and measure iNOS expression levels.

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3 protocols using mouse anti inos antibody

1

Immunostaining Protocol for Neurodegeneration

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Example 1

Maraviroc was purchased from Selleck Chemicals (Houston, Tex.). Mouse anti-TH antibody (Immunostar), rabbit anti-α-syn antibody (clone: MJFR1) (Abcam), anti-CD4 antibody (Thermofisher), anti-CD8 antibody (Thermofisher), anti-Iba1 antibody (Abcam), anti-GFAP antibody (Agilent), and mouse anti-iNOS antibody (BD Bioscience) were purchased from different vendors. Cy2- and Cy5-conjugated secondary antibodies were obtained from Jackson Immuno Research Laboratories (West Grove, Pa.).

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2

Ginsenoside Rg3 Neuroprotective Effects

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20(S)-Ginsenoside Rg3 from Panax ginseng (C42H72O13; formula weight, 785.01; purity, 95%~99%) was purchased from Biopurify Phytochemicals (Chengdu, China). Mouse anti-Bax and mouse anti-Bcl-2 antibodies for western blotting were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit anti-Bax antibody for immunohistochemistry was purchased from Abcam (Cambridge, UK). TACS 2 TdT-DAB in Situ Apoptosis Detection kit for terminal transferase dUTP nick-end labeling (TUNEL) assay was purchased from Trevigen (Gaithersburg, MD, USA). Mouse anti-iNOS antibody was purchased from BD Biosciences (Laguna Hills, CA, USA). Rabbit anti-COX-2 antibody was purchased from Cayman Chemical (Ann Arbor, MI, USA). Rabbit anti-ionized calcium binding adaptor molecule 1 (Iba1) antibody was purchased from Wako Pure Chemical Industries (Osaka, Japan). Mouse anti-β-actin antibody was purchased from Chemicon International (Temecula, CA, USA). Biotinylated anti-mouse and anti-rabbit secondary antibodies were purchased from Vector Laboratories (Burlingame, CA, USA). The remaining chemicals and reagents used were of analytical grade and were obtained from commercial sources.
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3

Immunohistochemistry of Synucleinopathy

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Maraviroc was purchased from Selleck Chemicals (Houston, TX). Mouse anti-TH antibody (Immunostar), mouse anti-human laminin α5 antibody clone # 4B12 (Millipore), rabbit anti-α-syn antibody (clone: MJFR1) (Abcam), anti-CD4 antibody (Thermofisher), anti-CD8 antibody (Thermofisher), anti-Iba1 antibody (Abcam), anti-GFAP antibody (Agilent), and mouse anti-iNOS antibody (BD Bioscience) were purchased from different vendors. Cy2- and Cy5-conjugated secondary antibodies were obtained from Jackson Immuno Research Laboratories (West Grove, PA).
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