20(
S)-Ginsenoside Rg3 from Panax ginseng (C
42H
72O
13; formula weight, 785.01; purity, 95%~99%) was purchased from Biopurify Phytochemicals (Chengdu, China). Mouse anti-Bax and mouse anti-Bcl-2 antibodies for western blotting were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit anti-Bax antibody for immunohistochemistry was purchased from Abcam (Cambridge, UK). TACS 2 TdT-DAB in Situ Apoptosis Detection kit for terminal transferase dUTP nick-end labeling (TUNEL) assay was purchased from Trevigen (Gaithersburg, MD, USA).
Mouse anti-iNOS antibody was purchased from BD Biosciences (Laguna Hills, CA, USA).
Rabbit anti-COX-2 antibody was purchased from Cayman Chemical (Ann Arbor, MI, USA). Rabbit anti-ionized calcium binding adaptor molecule 1 (Iba1) antibody was purchased from Wako Pure Chemical Industries (Osaka, Japan).
Mouse anti-β-actin antibody was purchased from Chemicon International (Temecula, CA, USA).
Biotinylated anti-mouse and anti-rabbit secondary antibodies were purchased from Vector Laboratories (Burlingame, CA, USA). The remaining chemicals and reagents used were of analytical grade and were obtained from commercial sources.
Kim D.K., Kweon K.J., Kim P., Kim H.J., Kim S.S., Sohn N.W., Maeng S, & Shin J.W. (2017). Ginsenoside Rg3 Improves Recovery from Spinal Cord Injury in Rats via Suppression of Neuronal Apoptosis, Pro-Inflammatory Mediators, and Microglial Activation. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(1), 122.