100 mg lung tissue was rinsed and homogenized and then stored overnight at −20°C. The samples were performed to break the cell membranes; the homogenates were centrifuged for 5 minutes at 5000μg at 2–8°C. The supernatant was removed. The double-antibody sandwich ELISA kits (eBioscience, USA) were used to detect the levels of tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), IL-6, IL-10, MPO, and TSG-6 in lung tissues and serum according to the manufacturer's protocols.
Double antibody sandwich elisa kits
Manufactured by Thermo Fisher Scientific
Sourced in United States
Double-antibody sandwich ELISA kits are laboratory products used for the detection and quantification of specific analytes in various sample types. These kits employ a sandwich-based immunoassay technique to capture and measure the target analyte.
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Lab products found in correlation
3 protocols using double antibody sandwich elisa kits
1
Lung Inflammatory Markers Quantification
2
Cytokine and Antibody Profiling in Allergic Co-Culture
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The activities of IL-4, IL-5, IL-13, IL-9, IL-21, IL-23, IL-17A, and IFN-γ in the supernatants of the co-culture medium were determined corresponding commercially available double-antibody sandwich ELISA kits and (all from eBioscience). Serum was isolated from whole blood, and the total IgE and IgG1 concentrations were measured by ELISA kits. OVA-Specific IgE and IgG1 were detected with the Mouse OVA-specific IgE ELISA Kit and Mouse OVA-specific IgG1 ELISA Kit (both from Biolegend). All operations were performed in accordance with the manufacturer’s instructions.
3
Cytokine Profiling in Cell Co-Cultures
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Referring to previous studies [28 (link),29 (link)], the concentrations of secreted IL-4, IL-5, IL-13, IL-9, IL-22, IL-21, IL-23, IL-17A, IL-17F, IFN-γ, IL-10, TGF-β, IL-25, and IL-33 in the supernatants of the co-cultures were determined using the respective commercially available double-antibody sandwich ELISA kits (all from eBioscience).
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