Neuro-2A (N2a, neuroblastoma cell line, ATCC CCL-131) and Sol8 (myoblast cell line, ATCC CRL-2174) cells were directly purchased from American Type Culture Collection (ATCC, Manassas, VA); BV2 cells (microglial cell line) were kindly provided by Dr. Wai Wong (National Eye Institute, NIH). All cells were cultured based on standard ATCC protocols. N2a, Sol8, and BV2 cells were plated in a 96-well plate 24 hours prior to treatment with recombinant TNF-Related Apoptosis-Inducing Ligand (TRAIL/Apo2L, PeproTech, Rocky Hill, NJ) diluted in phosphate-buffered saline (PBS) at various concentrations. Cell viability was quantified 24 hours after treatment using the MTT (3-[4,5-dimethylthiazol]-2,5-diphenyltetrazolium) assay (Thermo Fisher Scientific, Waltham, MA) based on manufacturer’s instructions. Each experiment was performed with n = 8, and was repeated at least three times. Data were expressed as percentage of cell death with respect to control untreated cells.
Trail apo2l
Manufactured by Thermo Fisher Scientific
Sourced in United States
TRAIL/Apo2L is a recombinant human protein that belongs to the tumor necrosis factor (TNF) superfamily. It functions as a ligand that can induce apoptosis, or programmed cell death, in various cell types.
Automatically generated - may contain errors
Lab products found in correlation
Neuro 2a, by American Type Culture Collection (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Thermo Fisher Scientific (1 mentions)
Anti lc3, by Novus Biologicals (1 mentions)
Anti pro caspase 3, by Cell Signaling Technology (1 mentions)
Beclin 1, by Cell Signaling Technology (1 mentions)
Bapta am, by Merck Group (1 mentions)
Pro caspase 8, by Cell Signaling Technology (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Sk n as, by American Type Culture Collection (1 mentions)
Xanthohumol, by Bio-Techne (1 mentions)
Sh sy5y, by American Type Culture Collection (1 mentions)
Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
4 protocols using trail apo2l
1
Cytotoxicity Assay of TRAIL on Neuronal and Muscle Cells
2
Investigating Autophagy Modulation in Cell Death
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
R-[2,3-Dihydro-5-methyl-3[(4-morpholinyl)methyl] pyrrolo[1,2,3,-de]-1,4-benzoxazin-6-yl]-1-naphthalenyl methanone mesylate (WIN55,212-2), anandamide (ANA), meth-anandamide (MethANA), 3-methyl-adenine (3-MA) and BAPTA-AM were purchased from Sigma, soluble human recombinant TRAIL/APO2L was obtained from PeproTech (EC Ltd., London, UK), benzyloxy-carbonyl-Val-Ala-Asp-fluoromethylketone (z-VAD-fmk) from Promega (Madison, WI). Stock solutions were prepared in DMSO and opportunely diluted in culture medium except for 3-MA which was prepared in ethanol. The final concentration of DMSO or ethanol never exceeded 0.04%, which is a concentration that was experimentally determined to have no discernible effect. All antibodies used were purchased from Santa Cruz Bio (Santa Cruz, CA, USA), except for anti-procaspase-3, procaspase-8, Beclin-1 and PARP (Cell Signalling, Beverly, MA, USA), anti-LC3 (Novus Biologicals, Cambridge, UK), p62 and Actin (Sigma, MI, Italy).
3
Neuroblastoma Cell Lines and Xanthohumol Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Three human neuroblastoma (NB) cell lines (SK-N-AS, NGP, and SH-SY-5Y) were used in this study, and SK-N-AS and SH-SY-5Y were purchased from ATCC. The NGP was a kind gift from Dr. Thiele. The cells were grown in Roswell Park Memorial Institute Media (RPMI; Life Technologies, Carlsbad, CA), supplemented with 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Life Technologies) in a humidified atmosphere of 5% CO2 in air at 37°C. Xanthohumol (Tocris, Minneapolis, MN or Selleckchem, Houston, TX) was dissolved in dimethyl sulfoxide (DMSO: Sigma-Aldrich) to prepare stock solutions of 50 mM. An equivalent volume of DMSO alone served as a negative control. TRAIL/Apo-2L was purchased from PeproTech (Rocky Hill, New Jersey, USA) and stored in aliquots at -80°C.
4
Preparation of Recombinant TRAIL/Apo2L
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human recombinant TRAIL/Apo2L was bought from Peprotech, USA and dissolved in 0.1% BSA (Sigma-Aldrich, St Louis, MO). After full dissolution, it was splited into small packages and stored at −80°C.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!