Transferrin

AML-12 mouse hepatocyte, a nontransformed cell line, was obtained from American Type Culture Collection (ATCC, Manassas, VA). AML-12 hepatocytes were cultured in Dulbecco's Modified Eagle Medium/Ham's Nutrient Mixture F-12 (DMEM/F12, 1 : 1, Hyclone) containing 10% (v/v) fetal bovine serum (FBS, Biological Industries, ISR), 5 mg/mL insulin (Solarbio, Beijing, China), 5 μg/mL transferrin (Solarbio, Beijing, China), 5 ng/mL selenium (Sigma-Aldrich, St. Louis, MO), and 40 ng/mL dexamethasone (Sigma-Aldrich, St. Louis, MO) at 37°C in a humidified atmosphere of 5% CO₂ and 95% air.

β-Galactosidase (β-Gal), horseradish peroxidase, alkaline phosphatase, papain, pepsin, and α-mannosidase were purchased from Beyotime (Shanghai, China). Lysozyme, glucose, bovine serum albumin, γ-globulin, transferrin, mannitol tryptophan and proline were purchased from Solarbio (Beijing, China). Ciprofloxacin, ampicillin, kanamycin, dimethyl sulfoxide (DMSO), Polypropylene pyrrolidone K30 (PVP-K30, MW = 40,000) and all other chemicals and solvents were purchased from Sigma-Aldrich (St. Louis, United States). Luria-Bertani broth (LB) medium was purchased from BD Biosciences (San Jose, CA). All aqueous solutions were prepared with Milli-Q water (≥18 MΩ, Milli-Q, Millipore).

Poly(d, l-lactide-co-glycolide) (Resomer® RG 504) and PVA (87–90% hydrolyze) were purchased from Sigma-Aldrich (UK), the anti-BCR/ABL rabbit antibody is an IgG subtype antibody and recognizes the ABL sequence of BCR/ABL oncoprotein (Sangon Biotech, China), Transferrin (≥ 98%) and Coumarin6(≥ 98%) were purchased from Solarbio (China).

For the fabrication of microcavity, the silica capillary was purchased from Polymicro Inc. (#TSP250350). Dimethyloctadecyl[3-(trimethoxysilyl)propyl] ammoniumchloride (DMOAP, #A-FF032) to modify the internal surface of the microcavity and 4-Cyano-4′-N-Pentylbiphenyl as nematic LC (5CB) were from Xianding Biotechnology Co., Ltd. (Shanghai, China, #C486A). When the wavelength is ranging from 1565 nm to 1575 nm, the extraordinary refractive index (n_e) of 5CB is 1.67 and the ordinary refractive index (n_o) of 5CB is 1.51. BSA (#PC0001from Solarbio Science & Technology, Co., Ltd., Beijing, China), transferrin (Solarbio, #T8010), human IgG (HIgG) (#bs-0297P from Bioss, Beijing, China), goat anti-human IgG (AHIgG) (Solarbio, #PA101), goat anti-human albumin (AHSA) (Solarbio, #SPA107) and goat anti-mouse IgG (AMIgG) (Solarbio, #SPA131) were used for protein assays as protein solutions. Olive oil (Solarbio, #O8320) was employed to explain the effects of absorption behavior on spectral responses.

Ammonium tetrachloropalladate (II) ((NH₄)₂PdCl₄, 98%) and dl-methionine (C₅H₁₁NO₂S, 99.9%) were obtained from Adamas-Beta (Shanghai, China). Ascorbic acid (C₆H₈O₆, 99.9%), human serum albumin (HSA), ribonuclease A (RNA), cytochrome C, transferrin, bovine serum albumin (BSA), lysozyme, and hemoglobin were obtained from Solarbio (Beijing, China). All competitive metal ions and glucose used for selectivity testing were acquired from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). All reagents were used as received without further purification. Ultrapure water (resistivity: 18.2 MΩ⋅cm) was obtained from a Millipore purification system.