Fasting blood was drawn intravenously from anesthetized rats into lithium-heparin coated blood tubes and transported immediately to the laboratory for analysis. Levels of triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL) were measured by the clinical laboratory service at Ruby Memorial Hospital (Morgantown, WV). Blood glucose was measured using a commercially available glucometer (FreeStyle, Abbott), and insulin was measured using a rat ELISA kit (Cayman Chemical, item #589501).
Rat elisa kit
Manufactured by Cayman Chemical
Sourced in United States
The Rat ELISA Kit is a laboratory assay used to quantify specific analytes in rat biological samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and measure target molecules. The kit provides the necessary reagents and protocols to perform the ELISA analysis.
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Lab products found in correlation
3 protocols using rat elisa kit
1
Rat Blood Analysis Protocol
2
Hippocampal Apoptosis and NF-kB Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
The plasma insulin concentration was determined by rat ELISA kit (Cayman chem., Ann Arbor, MI, USA; Cat. No: 589501). Also, the NF-kB activity in hippocampus was determined by measuring the phosphorylated NF-kB p65 levels with an ELISA kit (Cayman Chemical, Ann Arbor, MI), according to the manufacturer’s instructions. we measured apoptosis rate in this tissue using cell death detection ELISA kit (1544675, Roche, Germany) at 405 nm as previously described.18 (link) According to the manufacturer’s instructions, 20-mg sections from hippocampus were homogenized in 400 μl of hypotonic buffer for 15 min and centrifuged at 14,000×g for 10 min at 4 °C. The supernatant was used for determination apoptosis rate. The remaining nuclear pellet was resuspended in 100 μl of extract buffer for 10 min and centrifuged at 14,000×g for 10 min at 4 °C. The supernatant containing the nuclear fraction was used for quantification of NF-kB activity.
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The plasma insulin concentration was determined by rat ELISA kit (Cayman chem., Ann Arbor, MI, USA; Cat. No: 589501). Also, the NF-kB activity in hippocampus was determined by measuring the phosphorylated NF-kB p65 levels with an ELISA kit (Cayman Chemical, Ann Arbor, MI), according to the manufacturer’s instructions. we measured apoptosis rate in this tissue using cell death detection ELISA kit (1544675, Roche, Germany) at 405 nm as previously described.18 (link) According to the manufacturer’s instructions, 20-mg sections from hippocampus were homogenized in 400 μl of hypotonic buffer for 15 min and centrifuged at 14,000×g for 10 min at 4 °C. The supernatant was used for determination apoptosis rate. The remaining nuclear pellet was resuspended in 100 μl of extract buffer for 10 min and centrifuged at 14,000×g for 10 min at 4 °C. The supernatant containing the nuclear fraction was used for quantification of NF-kB activity.
3
Rat Blood Analysis Protocol
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