Ccl11 eotaxin 1

Cytokines were measured by enzyme-linked immunosorbent assay according to the manufacturer’s instructions. CCL11 (Eotaxin-1) and CCL24 (Eotaxin-2) ELISA kits were purchased from R&D Systems (Minneapolis, MN). IL-4 ELISA kit was purchased from BioLegend (San Diego, CA). CCL2 was obtained from Peprotech (Rechovot, IL) and Relm-α was detected as previously described^{59 (link)}. Lower detection limits for these assays were 15.625 pg/ml (CCL11) and 31.25 pg/ml (CCL24, CCL2 and Relm-α).

Alamar Blue cell viability reagent was purchased from Invitrogen (Massachusetts, USA). Dexamethasone, diclofenac sodium, dimethyl sulfoxide (DMSO), Dulbecco's Modified Eagle Medium (DMEM), ethylenediaminetetraacetic acid (EDTA), fetal bovine serum (FBS), Laemmli sample buffer, lipopolysaccharide (LPS), phosphate-buffered saline (PBS), and protease inhibitor cocktail were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ether, ethanol, ethyl acetate, dichloromethane, methanol, n-butanol, and n-hexane were purchased from Tedia Way (Fairfield, OH, USA). Nitrocellulose membranes were purchased from Amersham Pharmacia Biotech (San Francisco, CA, USA). The chromatographic solvents (acetonitrile, acetic acid, and trifluoroacetic acid) were purchased from Merck (Darmstadt, Germany). Monoclonal antibodies anti-COX-2, anti-goat IgG biotin-conjugated antibody, and streptavidin-conjugated horseradish peroxidase were purchased from Santa Cruz Biotechnology (Texas, USA). The CXCL2/MIP-2, CXCL1/KC, CCL11/eotaxin-1, and TNF-α enzyme-linked immunosorbent assay (ELISA) kit were purchased from R&D Systems (Minneapolis, MN, USA). The colorimetric kit used for the May-Grunwald-Giemsa method was purchased from Laborclin (Pinhais, PR, BR).