Immunohistochemical or immunocytochemical staining for 7 μm wax sections of ONHs or cultured ONH astrocytes were performed as described previously (Ju et al., 2008 (link)). Five sections per wax block from each group (n = 4 ONHs/group) were used for immunohistochemical analysis. The primary antibodies included mouse monoclonal anti-glial fibrillary acidic protein (GFAP) antibody (1:300; Sigma), guinea pig polyclonal anti-GFAP antibody (1:500; Advanced ImmunoChemical. Long Beach, CA), mouse monoclonal anti-neurofilament antibody (1:500; Sigma), mouse monoclonal anti-NR1 antibody (1:1000; BD Pharmingen, San Diego, CA), rabbit monoclonal anti-NR2A antibody (1:100; Millipore, Billerica, MA), rabbit polyclonal anti-NR2B antibody (1:5000; Millipore), and mouse monoclonal anti-DRP1 antibody (1:1000; BD Transduction Laboratories, San Diego, CA). The images were acquired with a FluoView1000 confocal microscope (Olympus, Tokyo, Japan).
Mouse monoclonal anti neurofilament antibody
Manufactured by Merck Group
The mouse monoclonal anti-neurofilament antibody is a laboratory reagent that can be used to detect and identify neurofilament proteins in biological samples. Neurofilaments are structural proteins found in neurons and are commonly used as markers for neuronal cells.
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Lab products found in correlation
Mouse monoclonal anti glial fibrillary acidic protein antibody, by Merck Group (2 mentions)
Mouse monoclonal anti nr1 antibody, by BD (2 mentions)
Rabbit monoclonal anti nr2a antibody, by Merck Group (2 mentions)
Rabbit polyclonal anti nr2b antibody, by Merck Group (2 mentions)
Mouse monoclonal anti drp1 antibody, by BD (2 mentions)
Fluoview 1000 confocal microscope, by Olympus (2 mentions)
In cell analyzer 1000, by GE Healthcare (1 mentions)
Saponin, by Merck Group (1 mentions)
3 protocols using mouse monoclonal anti neurofilament antibody
1
Immunostaining of Optic Nerve Head Cells
2
Immunohistochemical Analysis of ONH Astrocytes
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Immunohistochemical or immunocytochemical staining for 7 µm wax sections of ONHs or cultured ONH astrocytes were performed as described previously (Ju et al., 2008 (link)). Five sections per wax block from each group (n = 4 ONHs/group) were used for immunohistochemical analysis. The primary antibodies included mouse monoclonal anti-glial fibrillary acidic protein (GFAP) antibody (1:300; Sigma), guinea pig polyclonal anti-GFAP antibody (1:500; Advanced ImmunoChemical. Long Beach, CA), mouse monoclonal antineurofilament antibody (1:500; Sigma), mouse monoclonal anti-NR1 antibody (1:1,000; BD Pharmingen, San Diego, CA), rabbit monoclonal anti-NR2A antibody (1:100; Millipore, Billerica, MA), rabbit polyclonal anti-NR2B antibody (1:5,000; Millipore), and mouse monoclonal anti-DRP1 antibody (1:1,000; BD Transduction Laboratories, San Diego, CA). The images were acquired with a FluoView1000 confocal microscope (Olympus, Tokyo, Japan).
3
Neurite Length Quantification in Cells
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After permeabilization with saponin (Sigma), cells were blocked for 2 hr with PBS containing 10% goat serum. Then cells were fixed, and neurites detected by using mouse monoclonal anti‐neurofilament antibody (Sigma‐Aldrich, Cat# N4142, RRID:AB_477272) revealed by an anti‐mouse Alexa Fluor 488 (1:400, Invitrogen, cat#A32723, RRID:AB_2633275). Total neurite length was analyzed using In Cell Analyzer™ 1000 (GE Healthcare).
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