Hoechst 33342 stain

Manufactured by Dojindo Laboratories

Sourced in Japan

Hoechst 33342 is a fluorescent dye that binds to the minor groove of DNA. It is commonly used for staining and visualization of nuclei in live and fixed cells.

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Lab products found in correlation

In cell analyzer 2000, by GE Healthcare (1 mentions) Propidium iodide, by Dojindo Laboratories (1 mentions) Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions) Anti mouse alexa 488, by Thermo Fisher Scientific (1 mentions) Anti rabbit alexa488, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Hoechst 33342 (297 citations) Hoechst (13 citations)

2 protocols using hoechst 33342 stain

Blue Light Cytotoxicity Assay in ARPE-19 Cells

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ARPE-19 cells were pretreated with cultured supernatants from human PBMC-derived M2 macrophages stimulated with or without L. paracasei KW3110 at a concentration of 10 μg/mL. After 24 h cultivation, the cells were exposed to 1250 lux of blue light (LEDB-SBOXH; OptoCode Corp., Tokyo, Japan) at 470 nm for 50 min. In addition, ARPE-19 cells were also pretreated with phosphate-buffered saline (PBS) or IL-10 (10 ng/mL). After 24 h of cultivation, these cells were exposed to 1250 lux of blue light (LEDB-SBOXH, OptoCode Corp.) at 470 nm for 10 min. After 24 h of light exposure, the Hoechst 33342 stain (Dojindo, Tokyo, Japan) and propidium iodide (PI) (Dojindo) were added to the culture medium at final concentrations of 8.1 μM and 1.5 μM, respectively. PI-positive cells were determined as the ratio of PI-positive to Hoechst 33342-positive cells using an In Cell Analyzer2000 (GE Healthcare, Tokyo, Japan).

Morita Y., Jounai K., Miyake M., Inaba M, & Kanauchi O. (2018). Effect of Heat-Killed Lactobacillus paracasei KW3110 Ingestion on Ocular Disorders Caused by Visual Display Terminal (VDT) Loads: A Randomized, Double-Blind, Placebo-Controlled Parallel-Group Study. Nutrients, 10(8), 1058.

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Mitochondrial and Protein Localization Imaging

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Cells were cultured on coverslips and loaded with 50 nM MitoTracker® Red CMXROS (Invitrogen) for 30 min at 37 °C in the dark, then fixed in 2% paraformaldehyde for 10 min at 25 °C, and permeabilized with 0.2% Triton X-100 for 10 min at 4 °C. The cells were incubated with anti-V5 or anti-myc antibodies, followed by secondary anti-mouse Alexa 568, anti-mouse Alexa 488, or anti-rabbit Alexa 488 (Invitrogen). Cells were further incubated with Hoechst-33342 stain (Dojindo, Kumamoto, Japan) for 10 min at room temperature in the dark after being washed. All fluorescent images were acquired with the BIOTRVO BZ-9000 (Keyence, Osaka, Japan).

Hirasaka K., Mills E.M., Haruna M., Bando A., Ikeda C., Abe T., Kohno S., Nowinski S.M., Lago C.U., Akagi K.I., Tochio H., Ohno A., Teshima-Kondo S., Okumura Y, & Nikawa T. (2016). UCP3 is associated with Hax-1 in mitochondria in the presence of calcium ion. Biochemical and biophysical research communications, 472(1), 108-113.

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