Horseradish peroxidase hrp conjugated anti mouse and anti rabbit antibodies

Kaempferol (Fig. 1A) was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA) and dissolved in 100% dimethyl sulfoxide (DMSO). A stock solution of 100 mmol/L Kaempferol was prepared and stored as small aliquots at −20°C until use. We purchased 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), DMSO, gelatin, and horseradish peroxidase (HRP)-conjugated anti-mouse and anti-rabbit antibodies from Sigma-Aldrich Co.. Growth factor-reduced Matrigel was purchased from BD Biosciences (San Jose, CA, USA). The phospho-specific antibodies (anti-p38, anti-PI3K, anti-AKT, anti-mTOR, anti-p70S6K, anti-VEGFR2, and anti-HIF-1α) and the MAPK inhibitors (SB203580 and PD 98059) were purchased from Cell Signaling Technology (Danvers, MA, USA). The HRP-conjugated β-actin and phospho-ERK antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

DME was provided by Dr. Chul-yung Choi of Jeonnam Institute of Natural Resources Research. The compound was dissolved in 100% dimethyl sulfoxide (DMSO). A 100 mg/mL stock solution of DME was prepared and stored at −20°C until use. DMSO, MTT, rhodamine 123, verapamil, and horseradish peroxidase (HRP)-conjugated anti-rabbit and anti-mouse antibodies were purchased from Sigma-Aldrich. The apoptosis detection kit was purchased from BD Biosciences. 2′7′-dichlorofluorescein diacetate (H₂DCFDA) was purchased from Molecular Probes. Phospho-specific anti-p38, anti-FOXO3a, anti-PI3K, anti-AKT, anti-mTOR, and p70 antibodies; anti-PI3K, anti-AKT, anti-mTOR, p70, caspase-9, caspase-3, and PARP-specific antibodies; and the AKT inhibitor LY294002 were purchased from Cell Signaling Technology. HRP-conjugated β-actin, p53, p21, cyclin-dependent kinase (CDK) 4, cyclin D, CDK2, cyclin E, Bax, and Bcl-2 antibodies were purchased from Santa Cruz Biotechnology.

HMH (Fig. 1A; Sigma-Aldrich Co., St. Louis, MO, USA) was dissolved in 100% dimethyl sulfoxide (DMSO). A 50 mmol/L stock solution of HMH was prepared and stored as small aliquots at −20°C until needed. DMSO, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and horseradish peroxidase (HRP)-conjugated anti-rabbit and anti-mouse antibodies were purchased from Sigma-Aldrich, an apoptosis detection kit was purchased from BD Biosciences (Franklin Lakes, NJ, USA), and 2,7-dichlorofluorescein (DCF) diacetate (H₂DCFDA) was purchased from Molecular Probes (Invitrogen, Carlsbad, CA, USA). Phosphospecific anti-JNK, anti-p38, anti-ERK, anti-PI3K, anti-AKT, and anti-mTOR antibodies, anti-caspase-3, anti-caspase-9, anti-poly(ADP-ribose) polymerase (PARP), anti-Bax, anti-Bcl-2, anti-JNK, anti-p38, anti-ERK, anti-PI3K, anti-AKT, and anti-mTOR specific antibodies, the ERK inhibitor PD98059, and the AKT inhibitor LY294002 were purchased from Cell Signaling Technology (Danvers, MA, USA). HRP-conjugated b-actin, p53, p21, p-cdc2, cdc2, cyclin B1, and Rb antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

UA (Fig. 1A) was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). The compound was dissolved in 100% dimethyl sulphoxide (DMSO), and a 50 mmol/L stock solution was prepared and stored as small aliquots at −20°C until use. DMSO, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and horseradish peroxidase (HRP)-conjugated anti-rabbit and anti-mouse antibodies were purchased from Sigma-Aldrich Co.. Phycoerythrin-conjugated CD24 and fluorescein isothiocyanate (FITC)-conjugated CD44 antibodies were purchased from BD Biosciences (San Jose, CA, USA). The phospho-specific anti-p38, anti-PI3K, anti-AKT, anti-FoxO1/FoxO3a antibodies, and specific anti-p38, anti-PI3K, anti-AKT, anti-FoxO1/FoxO3a, ERK inhibitor PD98059, and AKT inhibitor LY294002 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). HRP-conjugated β-actin, p53, p21, CDK4, cyclin D, CDK2, and cyclin E antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).