SNHG20 small interference RNA (si-SNHG20) or its negative control (si-NC), miR-217 mimics and inhibitor (miR-217 and anti-miR-217) or its control (miR-NC and anti-miR-217) were synthesized by GenePharma Co., Ltd (Shanghai, China). The overexpression vector pcDNA 3.1 was acquired from Thermo Fisher Scientific. The above oligonucleotides and SNHG20 overexpression plasmid (pcDNA-SNHG20) or its negative control (pcDNA) were transfected into ovarian cancer cell lines (SKOV3 and A2780) using Lipofectamine™ 3000 transfection reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s suggested protocols.
Anti mir 217
Manufactured by GenePharma
Sourced in China
Anti-miR-217 is a laboratory reagent designed for research purposes. It functions as an inhibitor of miR-217, a microRNA involved in various cellular processes. Anti-miR-217 can be used to study the role of miR-217 in biological systems.
Automatically generated - may contain errors
Lab products found in correlation
Scramble mimic, by GenePharma (1 mentions)
Puromycin, by Thermo Fisher Scientific (1 mentions)
Dharmafect 1 reagent, by Horizon Discovery (1 mentions)
Ppackh1 lentivector packaging system, by System Biosciences (1 mentions)
Pcdna3, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Si nc, by GenePharma (1 mentions)
Mir nc, by GenePharma (1 mentions)
Sh mapk1, by GenePharma (1 mentions)
Mir 217 mimic, by GenePharma (1 mentions)
Sh crnde, by GenePharma (1 mentions)
Pcdna3.1 crnde, by GenePharma (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Si circrna, by GenePharma (1 mentions)
Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions)
4 protocols using anti mir 217
1
Modulation of Ovarian Cancer Pathways
2
Transfection and Stable Overexpression of miR-217 in Cervical Cancer Cells
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miR-217 mimics, scramble mimics, anti-miR-NC (negative control), and anti-miR-217 were synthesized by GenePharma (Shanghai, People’s Republic of China). miRNAs (10 nM) were transfected into cervical cancer cell using DharmaFECT1 Reagent (Dharmacon, Lafayette, CO, USA). To construct stably overexpressing miR-217 SiHa cell, lentiviral constructs (LentimiR™ miRNA precursor clones; System Biosciences, Palo Alto, CA, USA) expressing miR-217 or respective empty vector (miR-NC) were packaged using the pPACKH1 Lentivector Packaging System (System Biosciences) and were used to transfect into SiHa cell. Stable clones were selected using 1 µg/mL puromycin (Thermo Fisher Scientific).20 (link),21 (link)
3
Characterizing CRNDE and MAPK1 in HepG2 and Huh-7 Cells
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PcDNA3.1‐CRNDE, sh‐CRNDE, pcDNA3.1‐MAPK1, sh‐MAPK1, miR‐217 mimics, anti‐miR‐217 and negative control were provided by GenePharma (Shanghai, China). Transfection of HepG2 and Huh‐7 cells was conducted using Lipofectamine™ 2000 (Invitrogen, Carlsbad, CA, USA). Transfected cells were cultured in 6‐well plates. After 48‐h cultivation, the cells were collected for subsequent analyses.
4
Silencing CircRNA and miR-217 in Ishikawa Cells
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si-NC (negative control) sequence: UUCUCCGAACGUGUCACGUTT, si-circRNA (si-hsa_circ_0023404 #1–3; #3 sequence: GGUUCCUGCUAAUCUAUAATT, miR-217 or anti-miR-217 were synthesized by GenePharma (Shanghai, China). They were transfected in Ishikawa cells using Lipofectamine 3000 Reagent (Life Technologies, USA) and then culture in at 37 °C and 5% CO2 for 48–72 h.
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