ADMPCs were examined for quality at the time of cell freezing, cell thawing, and just before transplantation. Quality control tests included cell number, cell viability, cell purity, and infection tests. Cell number and viability were determined by staining with 0.4% (w/v) Trypan blue solution (Wako Pure Chemical Industries). Cell purity was analyzed by flow cytometry using FITC-labeled anti-CD45 antibody (BD Biosciences), FITC-labeled anti-CD105 antibody (Ancell Corporation, Bayport, MN, USA), FITC-labeled anti-CD166 antibody (Ancell Corporation), and FITC-labeled isotype control mouse IgG1 antibody (BD Biosciences). Data were collected with a FACSCalibur (BD Biosciences). The culture supernatant was collected by aspiration and used for infection tests including a sterility test, mycoplasma negative test, and endotoxin test.
Fitc labeled anti cd45 antibody
Manufactured by BD
Sourced in United States
The FITC-labeled anti-CD45 antibody is a laboratory reagent used for the identification and quantification of CD45-positive cells in various biological samples. It binds specifically to the CD45 antigen, which is expressed on the surface of most hematopoietic cells. The FITC (Fluorescein Isothiocyanate) fluorescent label allows for the detection and analysis of CD45-positive cells using flow cytometry or other fluorescence-based techniques.
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Lab products found in correlation
2 protocols using fitc labeled anti cd45 antibody
1
Quality Control of ADMPCs Transplant
2
Quantifying CD133+ EPCs in Rat Blood
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Fluorescence‐activated cell analysis was performed to determine the number of CD133+ EPCs in peripheral blood from sham and DOCA‐salt rats. Briefly, peripheral blood was incubated with a fluorescein isothiocyanate (FITC)‐conjugated CD133 antibody (Beckman Coulter, Fullerton, CA). FITC‐labeled anti‐CD45 antibody (BD Biosciences, San Jose, CA) was used for differential gating during flow analysis. FITC‐labeled IgG1a (Beckman Coulter) and phycoerythrin‐labeled IgG2b (Becton Dickinson, Franklin Lakes, NJ) served as the control for color compensation. Analysis was performed with an automated fluorescence‐activated cell counter (Beckman Coulter) in which 1 000 000 events were counted. The absolute cell counts of all measured components per 1 000 000 events in the lymphocyte gate were calculated.31 (link)
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