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1200 series instrument

Manufactured by Agilent Technologies
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The 1200 series instrument is a modular high-performance liquid chromatography (HPLC) system designed for analytical and preparative applications. It consists of various modules, including a pump, autosampler, column compartment, and detector, which can be configured to meet specific analytical requirements. The core function of the 1200 series instrument is to provide reliable and accurate separation, identification, and quantification of chemical compounds in complex samples.

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Lab products found in correlation

6230 hr esi tof massspectrometer, by Agilent Technologies (2 mentions) Microtof mass spectrometer, by Bruker (2 mentions) Rf 6000 spectrofluorophotometer, by Shimadzu (1 mentions) Black flat bottom 96 well microplates, by Corning (1 mentions) Luna c18 2, by Phenomenex (1 mentions) 6110 quadrupole, by Agilent Technologies (1 mentions) Zorbax eclipse xdb phenyl column, by Agilent Technologies (1 mentions) 6410 triple quadrupole mass spectrometer, by Agilent Technologies (1 mentions) C18 guard column, by Agilent Technologies (1 mentions) Zorbax sb c18 column, by Agilent Technologies (1 mentions) Isco combiflash companion, by Teledyne (1 mentions) Ltq orbitrap xl instrument, by Thermo Fisher Scientific (1 mentions) Mp 90 melting point system, by Mettler Toledo (1 mentions) 400 mhz spectrometer, by Bruker (1 mentions) Phosphorimager, by Bio-Rad (1 mentions) Freezone 2.5 freeze dryer, by Labconco (1 mentions) Fluoromax 3 spectrometer, by Horiba (1 mentions) Mcp 200 modular circular polarimeter, by Anton Paar (1 mentions) Chirascan spectropolarimeter, by Applied Photophysics (1 mentions) Avance 500 spectrometer, by Bruker (1 mentions)

17 protocols using 1200 series instrument

1

Spectroscopic and Chromatographic Analyses of Enzymatic Reactions

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3D scans were performed on a Shimadzu RF 6000 spectrofluorophotometer. Enzyme activity by UV, fluorescence measurements and OD600 determinations were performed using a Tecan Spark 10 M microplate reader. Enzyme activity characterization through conversions was performed by HPLC measurements, using an Agilent Technologies 1200 Series instrument equipped with autosampler. The UV irradiation of assay samples was performed in Corning 96-well Clear Flat Bottom UV-Transparent microplates using a handheld Analytik Jena UV lamp of 302 nm. The fluorescence measurement was carried out in Corning 96-well Black Flat Bottom microplates. Styrene was purchased from Sigma Aldrich and solvents (n-hexane, n-octane, dimethyl sulfoxide, acetonitrile) of HPLC grade were purchased from VWR. The synthesis of fluorogenic probe 4 was performed according to reported procedure47 (link).
Moisă M.E., Amariei D.A., Nagy E.Z., Szarvas N., Toșa M.I., Paizs C, & Bencze L.C. (2020). Fluorescent enzyme-coupled activity assay for phenylalanine ammonia-lyases. Scientific Reports, 10, 18418.
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2

HPLC Separation and Analysis of Compounds

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Active extracts were dissolved in methanol and filtered using a Nylon Target Syringe Filter (0.45 µm pore size). Chromatographic separation was performed with an Agilent 1200 series instrument (Santa Clara, CA, USA) consisting of a quaternary pump, a degasser, a thermostatted column compartment, a photodiode-array detector, a high-performance auto sampler, and a fraction collector, all controlled by Agilent ChemStation ver. B.03.02 software. The column used was Luna C18(2) (Phenomenex, 150 × 4.6 µm, 3 µm particle size, 100 Å pore size) maintained at 40 °C. HPLC solvent A consisted of water–acetonitrile 95:5 and solvent B consisted of acetonitrile–water 95:5, both acidified with 0.1% formic acid. The flow rate was maintained at 0.5 mL/min with the following standard gradient: 0 min, 0% B; 30 min, 100% B; 35 min, 100% B; 36 min, 0% B and 5 min of equilibration. UV traces were monitored at 210, 235, 254, 280, 330 and 440 nm.
Trinh B.T., Jäger A.K, & Staerk D. (2017). High-Resolution Inhibition Profiling Combined with HPLC-HRMS-SPE-NMR for Identification of PTP1B Inhibitors from Vietnamese Plants. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(7), 1228.
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3

Lipid Nanodisc Analysis by LC-MS

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LC-MS analysis was conducted on lipid nanodiscs using Agilent Technologies 1200 Series Instrument with a G1316A variable wavelength detector set at λ = 210 nm, 1200 Series ELSD, 6110 quadrupole ESI-MS, using an Agilent Zorbax Eclipse XDB-Phenyl column (3 × 100 mm, 3.5 μm particle size, flow rate 1 mL/min, the mobile phases 0.05% formic acid in water and 0.05% formic acid in acetonitrile).
Zhang A.H., Edwards I.A., Mishra B.P., Sharma G., Healy M.D., Elliott A.G., Blaskovich M.A., Cooper M.A., Collins B.M., Jia X, & Mobli M. (2019). Elucidating the Lipid Binding Properties of Membrane-Active Peptides Using Cyclised Nanodiscs. Frontiers in Chemistry, 7, 238.
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4

Norepinephrine Identification in Cocultures

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The norepinephrine
standard and the coculture
extract were analyzed on an Agilent 1200 series instrument equipped
with a DAD for retention time matching. Under aqueous normal phase
conditions, 10 μL of (1) a 1 mM norepinephrine standard, (2)
the coculture extract, and (3) an equal mixture of the two were injected
onto a Luna 5 μm HILIC column (150 × 4.60 mm; Phenomenex),
with a security guard (Phenomenex). Solvent A was 30 mM ammonium acetate,
pH = 4, and solvent B was ACN + 0.02% FA. The gradient was 5–15%
A over 20 min, at 1 mL/min. UV monitoring was performed at 210, 250,
254, and 270 nm.
Zink K.E., Dean M., Burdette J.E, & Sanchez L.M. (2018). Imaging Mass Spectrometry Reveals Crosstalk between the Fallopian Tube and the Ovary that Drives Primary Metastasis of Ovarian Cancer. ACS Central Science, 4(10), 1360-1370.
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5

Metabolite Profiling of Hairy Roots

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Dried hairy roots (50 mg) were ground into a fine powder and extracted twice with 25 mL of 80% methanol under sonication for 30 min. After centrifugation, the supernatant was diluted with 80% methanol to a total volume of 50 mL, and filtered through a 0.22 μm organic membrane filter prior to HPLC analysis. HPLC analysis was conducted on an Agilent 1200 series instrument with an Agilent 6410 triple-quadrupole mass spectrometer and an electrospray ionization source (Agilent Corporation, MA, USA). Metabolite separation was achieved on an Agilent ZORBAX SB-C18 column (3.5 μm, 2.1 × 150 mm) and an Agilent C18 guard column (5 μm, 4.0 × 2.0 mm). The mobile phase was acetonitrile: 5 mM ammonium acetate solution (the concentration of acetonitrile was from 5 to 95% in 1.0 min, v/v) with the flow rate of 0.3 mL·min−1 and a total run time of 5 min. Metabolite identification and quantification was achieved in multiple reaction monitoring mode (MRM). Characteristic m/z ions are listed in Table S2. The samples for qRT-PCR and metabolites analysis were the same.
Chen R., Li Q., Tan H., Chen J., Xiao Y., Ma R., Gao S., Zerbe P., Chen W, & Zhang L. (2015). Gene-to-metabolite network for biosynthesis of lignans in MeJA-elicited Isatis indigotica hairy root cultures. Frontiers in Plant Science, 6, 952.
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6

Synthesis and Purification of Diverse Compounds

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All starting materials were purchased from commercial sources and used directly without further purification. Purification by flash column chromatography was done using a Teledyne ISCO Combiflash Companion instrument using Teledyne Redisep normal phase columns. 1H and 13C NMR spectra were recorded on a Mercury-300, VNMR-400, INOVA-400, INOVA-500, or INOVA-600 NMR spectrometer. Chemical shifts were reported in ppm and referenced to the residual deuterated solvent. Reactions were monitored by thin-layer chromatography on pre-coated aluminum plates (silica gel 60 F254, 0.25 mm) or liquid chromatography–mass spectrometry (LCMS) on an Agilent Technologies 1200 series instrument. High-resolution mass spectra were recorded on a VG 70-S Nier Johnson or JEOL instrument by the Emory University Mass Spectroscopy Center. Purity was established via LCMS (Varian) in at least two solvent systems (MeOH:water/ACN:water or MeOH:water/MeOH:water) unless otherwise noted. All compounds described were >95% pure by LCMS besides one compound (12k) that was >85% pure. Optical rotations were established using a PerkinElmer 314 instrument.
Epplin M.P., Mohan A., Harris L.D., Zhu Z., Strong K.L., Bacsa J., Le P., Menaldino D.S., Traynelis S.F, & Liotta D.C. (2020). Discovery of Dihydropyrrolo[1,2-a]pyrazin-3(4H)-one-Based Second-Generation GluN2C- and GluN2D-Selective Positive Allosteric Modulators (PAMs) of the N-Methyl-d-Aspartate (NMDA) Receptor. Journal of medicinal chemistry, 63(14), 7569-7600.
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7

HPLC Analysis of MITO-DCA Purity

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Example 6

HPLC studies were carried out using an Agilent 1200 series instrument to explore the purity of the samples. A 5 μL of 5 mM solution of MITO-DCA in DMSO was injected using a Zoebax C18 column and a 50:50 acetonitrile:isopropanol-1% trifluoroacetic acid (TFA) as mobile phase. The wavelength used for these experiments was 268 nm. HPLC showed a single peak confirming purity of the compounds.

US10004809B2. Precise delivery of therapeutic agents to cell mitochondria for anti-cancer therapy (2018-06-26). UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC. [US]. Inventors: Shanta Dhar [US], Rakesh Pathak [US].
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8

Analytical Methods for Bioprocessing

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The concentration
of residual glucose was determined using the SBA-40C
biosensor analyzer (Shandong Academy of Sciences, China).47 (link) The cell density was determined by measuring
the optical density at 562 nm (OD562) using a UV-1800 spectrophotometer
(Beijing Ruili Analytical Instrument Co., Ltd, China). The levels
of amino acids were analyzed by HPLC on a 1200 series instrument (Agilent,
USA) according to the method reported by Kőrös et al.48 (link)
Han G., Xu N., Sun X., Chen J., Chen C, & Wang Q. (2020). Improvement of l-Valine Production by Atmospheric and Room Temperature Plasma Mutagenesis and High-Throughput Screening in Corynebacterium glutamicum. ACS Omega, 5(10), 4751-4758.
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9

Characterization of Synthetic Compounds

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1H NMR and 13C NMR spectra were recorded on a Bruker 400 MHz spectrometer (Bruker, Ettlingen, Germany). Melting point was determined with an MP90 melting point system (Mettler-Toledo AG, Switzerland). High-resolution mass spectra were recorded using an LTQ Orbitrap XL instrument (Thermo Fisher Scientific, San Jose, CA, USA). Analytical high-performance liquid chromatography (HPLC) was performed on an Agilent Technologies 1200 Series Instrument. The compound was analyzed using an Eclipse XDB-C18 (4.6×250 mm, 5 µm) under a 254 nm UV detector. Methanol - water (85:15) was used as mobile phase at a flow rate of 1mL/min.
Cui A.L., Sun W.F., Zhong Z.J., Jin J., Xue S.T., Wu S., Li Y.H, & Li Z.R. (2020). Synthesis and Bioactivity of N-(4-Chlorophenyl)-4-Methoxy-3-(Methylamino) Benzamide as a Potential Anti-HBV Agent. Drug Design, Development and Therapy, 14, 3723-3729.
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10

Characterization of Small Molecules by Multifaceted Analytical Techniques

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NMR spectra were
recorded on a Jeol
ECA 500 MHz spectrometer. Small molecule mass spectra (MS) were recorded
at the University of California, San Diego, Chemistry and Biochemistry
Mass Spectrometry Facility, utilizing an Agilent 6230 HR-ESI-TOF mass
spectrometer. Reverse-phase HPLC (Vydac C18 column) purification and
analysis were carried out using an Agilent 1200 series instrument.
Products were lyophilized utilizing a Labconco FreeZone 2.5 freeze
dryer.
Polyacrylamide gels containing radiolabeled RNA were
analyzed by using a BioRad phosphorimager. Steady-state fluorescence
experiments were carried out in a microfluorescence cell (125 μL)
with a path length of 1.0 cm (Hellma GmbH & Co. KG, Müllheim,
Germany) on a Horiba Jobin Yvon (FluoroMax-3) spectrometer. Mass spectra
for oligonuceotides were obtained on a ThermoFinnigan LCQ DECA XP
at TriLink Biotechnologies, Inc.
McCoy L.S., Shin D, & Tor Y. (2014). Isomorphic Emissive GTP Surrogate Facilitates Initiation and Elongation of in Vitro Transcription Reactions. Journal of the American Chemical Society, 136(43), 15176-15184.
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