Culture flasks and 96 well plates flat bottom

Fluorescent dye thioflavin T (ThT) “UltraPure Grade” (AnaSpec, Fremont, CA, USA), isopropyl-beta-D-1-thiogalactopyranoside (IPTG; Fluka, Switzerland), buffer components, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) from Sigma (USA) were used without further purification. For amyloid preparation, Aβ40 and Aβ42 peptides from GL Biochem, Shanghai, China, and 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) from Sigma-Aldrich, St. Louis, MO, USA, were used. DMEM (glucose 4.5 g/L), fetal bovine serum (FBS), 0.25% trypsin-EDTA were purchased from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). Culture flasks and 96-well plates (flat bottom) were purchased from Corning (New York, NY, USA). Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), penicillin, and streptomycin were purchased from Gibco BRL (Life Technologies, Paisley, Scotland). The human cervical cancer cell line (HeLa) was obtained from the cell bank (The Center of Collective Usage “Collection of Vertebrate Cell Cultures”, Institute of Cytology, RAS)). 3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), rhodamine 123, and propidium iodide were purchased from Sigma-Aldrich (St. Louis, MO, USA).

The fluorescent dyes thioflavin T (ThT) “UltraPure Grade” (AnaSpec, Fremont, CA, USA) and 8-anilino-1-naphthalene sulfonate (ANS) from Serva (Heidelberg, Germany), isopropyl-beta-D-1-thiogalactopyranoside (IPTG; Fluka, Buchs, Switzerland), guanidine hydrochloride (GdnHCl), lysozyme and buffer components, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) from Sigma (Saint Louis, MO, USA) were used without further purification. The Dulbecco’s Modified Eagle Medium (DMEM, glucose 4.5 g/L), fetal bovine serum (FBS), 0.25% Trypsin-EDTA were from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). The culture flasks and 96-well plates (flat bottom) were from Corning (USA).

Guanidine hydrochloride (GdnHCl), lysozyme, buffer components, trypsin from bovine pancreas, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma (St. Louis, MO, USA). Thioflavin T (ThT) “UltraPure Grade” was supplied by AnaSpec (Fremont, CA, USA), and 8-anilino-1-naphthalene sulfonate (ANS) was from Serva (Heidelberg, Germany). Further purification of chemicals has not been performed. Reagents for cell cultivation, including DMEM medium (glucose 4.5 g/L), fetal bovine serum (FBS), and 0.25% Trypsin-EDTA were acquired from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). Culture flasks and 96-well plates (flat bottom) were from Corning (New York, NY, USA).