Ab13970

Manufactured by Rockland Immunochemicals

Ab13970 is a primary antibody developed by Rockland Immunochemicals. It is designed for use in various immunoassay and immunohistochemistry applications. The antibody is purified and provided in a liquid format.

Automatically generated - may contain errors

Lab products found in correlation

Lsm 710 confocal microscope, by Zeiss (3 mentions) Rabbit anti rfp, by Rockland Immunochemicals (2 mentions) Alexa fluor 488 goat anti chicken, by Thermo Fisher Scientific (2 mentions) Ab64883, by Abcam (1 mentions) Slide scanner, by Hamamatsu Photonics (1 mentions) Ab7778, by Abcam (1 mentions) Elastin, by Abcam (1 mentions) Collagen 3, by Abcam (1 mentions) A1 upright confocal microscope, by Nikon (1 mentions) Click it edu imaging kit, by Thermo Fisher Scientific (1 mentions) Fab3688g, by R&D Systems (1 mentions) Af954, by R&D Systems (1 mentions) Sca 1, by R&D Systems (1 mentions) Af1226, by R&D Systems (1 mentions) Pdgfra, by R&D Systems (1 mentions) Prb 155p, by Abcam (1 mentions) Af1062, by Abcam (1 mentions) Phospho histone h3 ser10 antibody, by Cell Signaling Technology (1 mentions) Alexa fluor 488 goat anti rabbit, by Olympus (1 mentions) Mab377, by Abcam (1 mentions)

6 protocols using ab13970

Immunostaining of Skin Sections

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Paraffin- or optimal cutting temperature compound-embedded tissues were sectioned and stained6 (link)7 (link) using the following primary antibodies (all diluted 1:100 unless stated otherwise) for immunofluorescence labelling: Lrig: R&D Systems, FAB3688G; CD26: R&D Systems, AF954; Sca1: R&D Systems, AF1226; PDGFRa: R&D Systems, AF1062; Collagen III: Abcam, ab7778, Collagen11a1: Abcam, ab64883; Elastin: Abcam, ab21610; Caveolin: Cell Signaling Technology, 3267; phospho-Histone H3 (Ser10) antibody: Cell Signalling Technology, 970; Active Caspase-3: RnD Systems, AF835; K14: Covance, PRB-155P, 1:500; GFP: Abcam, ab13970, 1:500; RFP: Rockland, 600-401-379, 1:300. EdU staining was performed with a Click-it EdU imaging kit (Invitrogen) according to the manufacturer's recommendations. Images were acquired with a Nikon A1 Upright Confocal microscope. Images of H&E- and Herovici-stained sections were acquired with a Hamamatsu slide scanner. Representative images of skin from two to three independent experiments with at least three biological replicates per group are shown.

Lichtenberger B.M., Mastrogiannaki M, & Watt F.M. (2016). Epidermal β-catenin activation remodels the dermis via paracrine signalling to distinct fibroblast lineages. Nature Communications, 7, 10537.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Mouse Brain

Check if the same lab product or an alternative is used in the 5 most similar protocols

Both male and female mice (9–14 weeks old) were
transcardially perfused with 0.1 M phosphate buffered saline (PBS) followed
by 10% formalin. Brains were dissected out and post-fixed in 10% formalin
for several hours and then cryoprotected in 30% sucrose (0.1 M PBS). Serial
40 μm sections were collected and incubated with the following
primary antibodies overnight at 4°C: rabbit anti-S100β
(1:1,000; Abcam, ab41548), mouse anti-NeuN (1:1,000; Millipore, MAB377),
chicken anti-GFP (1:1,000; Abcam, ab13970), rabbit anti-RFP (1:1,000;
Rockland, 600–401-379), chicken anti-RFP (1:1,000; a gift from Dr.
Brecha’s laboratory at UCLA), rabbit anti-GAT-3 (1:500; a gift from
Dr. Brecha’s laboratory at UCLA), rabbit antiKir4.1 (1:1,500;
Alomone, APC-035), rabbit anti-DARPP-32 (1:200; Abcam, ab40801) or mouse
anti-HA (1:1,000; Covance, MMS-101R). Sections were then incubated with the
following secondary antibodies (1:1,000; Molecular Probes): Alexa Fluor 488
goat anti-chicken (A11039), Alexa Fluor 488 goat anti-rabbit (A11008), Alexa
Fluor 546 goat anti-mouse (A11003), Alexa Fluor 546 goat anti-chicken
(A11040) and Alexa Fluor 594 goat anti-rabbit (R37007) at room temperature
for 2 h. Fluorescence imaging was acquired with a 40× oil-immersion
objective lens (NA 1.3) under a laser-scanning confocal microscope
(Olympus).

Yu X., Taylor A.M., Nagai J., Golshani P., Evans C.J., Coppola G, & Khakh B.S. (2018). Reducing astrocyte calcium signaling in vivo alters striatal microcircuits and causes repetitive behavior. Neuron, 99(6), 1170-1187.e9.

+ Open protocol

+ Expand

Immunohistochemistry for Fluorescent Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

IHC. Tissue was processed for GFP, mCherry, dsRed and/or c-Fos (FOS-IR) IHC as previously described (D’Agostino et al., 2016 ). Briefly, mice were transcardially perfused with phosphate buffered saline (PBS) followed by 10% neutral buffered formalin (Sigma-Aldrich). Brains were extracted, post-fixed in 10% neutral buffered formalin at 4°C, cryoprotected in 20% sucrose at 4°C and then sectioned coronally on a freezing sliding microtome at 25 μm. Tissue was processed for chicken anti-GFP (1:1000; AbCam, ab13970), rabbit anti-dsRED (1:1000; Rockland, 600-401-379), goat anti-mCherry/RFP (1:1000; Sicgen, AB0040-200) or anti-c-FOS (1:5000, rabbit, Calbiochem, PC38) primary antibodies and a biotinylated donkey anti-rabbit (1:500, Jackson ImmunoResearch Laboratories, Inc.) or Alexa Fluor (1:500, Life Technologies) secondary antibodies using standard protocols previously described (Lam et al., 2009 (link), Heisler et al., 2006 (link)). Tissue was then mounted on slides, cover slipped and the NTS visualized using an Axioskop II microscope (Carl Zeiss, Oberkochen, Germany) and Adobe Photoshop CS5 software. Images of single-label immuoreactivity (IR) for GFP or mCherry were used to visualize and analyze NTS injection sites.

D'Agostino G., Lyons D., Cristiano C., Lettieri M., Olarte-Sanchez C., Burke L.K., Greenwald-Yarnell M., Cansell C., Doslikova B., Georgescu T., Martinez de Morentin P.B., Myers MG J.r., Rochford J.J, & Heisler L.K. (2018). Nucleus of the Solitary Tract Serotonin 5-HT2C Receptors Modulate Food Intake. Cell Metabolism, 28(4), 619-630.e5.

+ Open protocol

+ Expand

In Vivo Tracing of Cre-Expressing Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

AAV-U6-CARMIL3 Cterm sg1-HITI-smFP-P2A-mCherry-SynI-Cre virus was intracortically infused in P0 Cas9 KI mice as described above. Mice were perfused at P14 and brains were cryosectioned to 40 μm sections. Brain slices were then permeabilized for 2 h in 0.2% Triton X-100 and then blocked for 2 h in blocking buffer (Abcam, ab1265870). Slices were then incubated in primary antibodies: Rat Anti-HA (Sigma, 3F10, 1:200), Chicken Anti-GFP (Abcam, ab13970, 1:1000), and Rabbit anti-RFP (Rockland, 600-041-379, 1:1000) for 72 h. Slices were then washed three times in PBS for hour each wash and incubated in secondary antibodies: Alexa Fluor 647 Goat anti-Rat (ThermoFisher, A-21247, 1:1000), Alexa Fluor 488 Goat anti-Chicken (ThermoFisher, A-11006, 1:1000), and Alexa Fluor 555 Goat anti-Rabbit (ThermoFisher, A32732, 1:1000) for 24 h. Slices were then washed three times in PBS for hour each wash and mounted on slides. Slices were imaged and tiled on a Zeiss LSM 710 confocal microscope.

Spence E.F., Dube S., Uezu A., Locke M., Soderblom E.J, & Soderling S.H. (2019). In vivo proximity proteomics of nascent synapses reveals a novel regulator of cytoskeleton-mediated synaptic maturation. Nature Communications, 10, 386.

+ Open protocol

+ Expand

Immunolabeling of Cortical Neurons

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cerebral cortical sections and neurons were immunolabeled as previously described (Guo et al., 2017 ; Schmid et al., 2003 (link); Weimer et al., 2008 (link); Yokota et al., 2007 (link)) with the following primary antibodies: anti-GFP (chicken, 1:1000; Abcam, ab13970), anti-RFP (rabbit, 1:500; Rockland, cat. nr. 600–401-379), anti-ACIII (rabbit polyclonal, 1:100; Santa Cruz, sc-56855), anti-Tau (mouse 1:1000, Millipore, MAB3420), anti-Map2 (chicken polyclonal, 1:1000, Abcam, ab5392), Immunoreactivity was detected by incubation with appropriate AlexaFluor 488 or Cy3-conjugated secondary antibodies (Invitrogen and Jackson ImmunoResearch). AlexaFluor 488, 647 or Cy3-conjugated (Invitrogen and Jackson ImmunoResearch) secondary antibodies were used. Nuclei were counterstained with DAPI (Sigma).

Guo J., Otis J., Suciu S.K., Catalano C., Xing L., Constable S., Wachten D., Gupton S., Lee J., Lee A., Blackley K.H., Ptacek T., Simon J.M., Schurmans S., Stuber G.D., Caspary T, & Anton E.S. (2019). Primary cilia signaling promotes axonal tract development and is disrupted in Joubert Syndrome Related Disorder models. Developmental cell, 51(6), 759-774.e5.

+ Open protocol

+ Expand

Dual Immunofluorescence Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary antibodies used for the first round are: mouse anti-Fasciclin II (DSHB, 1D4), 1:20; Chicken anti-GFP (abcam, ab13970), 1:400; Rabbit anti-RFP (Rockland), 1:400. Conjugated secondary antibodies used for the first round are: Goat anti-mouse Alexa-405 (A31553, Thermo Fisher Scientific), 1:250; Goat anti-chicken Alexa-488 (A32931, Thermo Fisher Scientific), 3:500; Donkey anti-rabbit Alexa-568 (A10042, Thermo Fisher Scientific), 1:500. Primary antibody used for the second round is: mouse anti-Chaoptin (DSHB, 24B10), 1:20. Conjugated secondary antibody used for the second round is: Donkey anti-mouse Alexa-647 (A31571, Thermo Fisher Scientific), 1:100.

Ji W., Wu L.F., & Altschuler S.J. (2020). On the role of photoreceptor identity in controlling accurate wiring of theDrosophilavisual circuit.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!