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Ma5 13065

Manufactured by Thermo Fisher Scientific
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The MA5-13065 is a laboratory equipment product from Thermo Fisher Scientific. It is designed for general laboratory use. The core function of this product is to perform a specific task or operation in a laboratory setting. However, further details about its intended use or specific capabilities are not available at this time.

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Lab products found in correlation

Ac 40, by Merck Group (2 mentions) Sc 816, by Santa Cruz Biotechnology (2 mentions) Sc 166394, by Santa Cruz Biotechnology (2 mentions) Sc 67060, by Santa Cruz Biotechnology (2 mentions) Sc 20681, by Santa Cruz Biotechnology (2 mentions) Ma1 310, by Thermo Fisher Scientific (2 mentions) Sc 32233, by Santa Cruz Biotechnology (2 mentions) Hrp conjugated goat anti rabbit, by Jackson ImmunoResearch (1 mentions) Hrp conjugated goat anti mouse antibody, by Jackson ImmunoResearch (1 mentions) Hrp conjugated goat anti human antibody, by Jackson ImmunoResearch (1 mentions) Ab8227, by Abcam (1 mentions) Whsc1l1 antibody, by Proteintech (1 mentions) H3k36me2, by Abcam (1 mentions) H3k4me3, by Merck Group (1 mentions) H3k36me3, by Abcam (1 mentions) Total h3, by Abcam (1 mentions)

4 protocols using ma5 13065

1

Antibody Sources for Protein Analysis

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The rabbit anti-human PAD2 and PAD4 antibodies were obtained from Proteintech (12110–1-AP; Rosemont, IL) and Sigma-Aldrich (P4749; St. Louis, MO), respectively. The rabbit anti-human caspase-3, activating transcription factor 4 (ATF4), 78 kDa Glucose-regulated Protein (GRP78) and beta actin antibodies were obtained from Abcam (ab4051; Cambridge, UK), Santa Cruz Biotechnology (sc-200; Dallas, TX), LS Bio (LS-C312961; Seattle, WA), and Abcam (ab8227), respectively. The mouse anti-dog/cat estrogen receptor alpha (ERα) antibody was obtained from Thermo Fisher Scientific (MA5–13065; Waltham, MA). The human anti-modified citrulline antibody was obtained from Millipore (MABS487; Darmstadt, Germany). Alexa 488-conjugated goat anti-rabbit antibodies, HRP-conjugated goat anti-mouse antibodies, HRP-conjugated goat anti-rabbit and HRP-conjugated goat anti-human antibodies were all obtained from Jackson ImmunoResearch Labs (West Grove, PA). The PAD inhibitor, BB-CLA, was synthesized as previously described and diluted in dimethyl sulfoxide (DMSO) [25 (link)].
Ledet M.M., Anderson R., Harman R., Muth A., Thompson P.R., Coonrod S.A, & Van de Walle G.R. (2018). BB-Cl-Amidine as a novel therapeutic for canine and feline mammary cancer via activation of the endoplasmic reticulum stress pathway. BMC Cancer, 18, 412.
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2

Protein Expression and Interaction Analysis

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20 µg of protein aliquots from cell lysates of testis from V7BAC transgenic mice were separated by 15% SDS-PAGE and transferred to nitrocellulose membranes. The membranes were probed with primary antibodies against VAMP7 (sc-166394, 1:100, Santa-Cruz or sc-67060, 1:50, Santa-Cruz), AR (sc-816, 1:1,000, Santa-Cruz), ESR1 (MA1-310, 1:200, Thermo-Fisher), LMNA (sc-20681, 1:200, Santa-Cruz), GAPDH (sc-32233, 1:200, Santa-Cruz), and ACTB (AC-40, 1:5,000, Sigma). HeLa cells were cotransfected with AR or ESR1 or VAMP7 or in combination. After 24 h of overexpression, cell lysates were prepared and immunoprecipitation was carried out using antibodies raised against AR (sc-816, Santa-Cruz) or ESR1 (MA5-13065, Thermo-Fisher) or VAMP7 (sc-67060, Santa-Cruz) or control IgG antibody. The samples were later subjected to western blotting with the indicated antibodies. Input was 5% of the lysates.
Tannour-Louet M., Han S., Louet J.F., Zhang B., Romero K., Addai J., Sahin A., Cheung S.W, & Lamb D.J. (2014). Increased gene copy number of the vesicle SNARE VAMP7 disrupts human male urogenital development through altered estrogen action. Nature medicine, 20(7), 715-724.
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3

Protein Expression and Interaction Analysis

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20 µg of protein aliquots from cell lysates of testis from V7BAC transgenic mice were separated by 15% SDS-PAGE and transferred to nitrocellulose membranes. The membranes were probed with primary antibodies against VAMP7 (sc-166394, 1:100, Santa-Cruz or sc-67060, 1:50, Santa-Cruz), AR (sc-816, 1:1,000, Santa-Cruz), ESR1 (MA1-310, 1:200, Thermo-Fisher), LMNA (sc-20681, 1:200, Santa-Cruz), GAPDH (sc-32233, 1:200, Santa-Cruz), and ACTB (AC-40, 1:5,000, Sigma). HeLa cells were cotransfected with AR or ESR1 or VAMP7 or in combination. After 24 h of overexpression, cell lysates were prepared and immunoprecipitation was carried out using antibodies raised against AR (sc-816, Santa-Cruz) or ESR1 (MA5-13065, Thermo-Fisher) or VAMP7 (sc-67060, Santa-Cruz) or control IgG antibody. The samples were later subjected to western blotting with the indicated antibodies. Input was 5% of the lysates.
Tannour-Louet M., Han S., Louet J.F., Zhang B., Romero K., Addai J., Sahin A., Cheung S.W, & Lamb D.J. (2014). Increased gene copy number of the vesicle SNARE VAMP7 disrupts human male urogenital development through altered estrogen action. Nature medicine, 20(7), 715-724.
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4

Antibody Procurement for Chromatin Immunoprecipitation

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The WHSC1L1 antibody was purchased from ProteinTech Group Inc. (Cat. No.
11345-1-AP). The estrogen receptor alpha antibody was purchase from Bethyl Labs
(Cat. No. A300-498A). ChIP-grade anti-estrogen receptor alpha antibodies were
purchased from Santa Cruz (sc-543x) and Thermo Scientific (MA5-13065).
ChIP-grade histone antibodies were used for both ChIP and immunoblotting, and
were purchased from Abcam and Millipore: total H3 (Abcam ab1791), H3K36me3
(Abcam ab9050), H3K36me2 (Abcam ab9049), and H3K4me3 (Millipore 17-678).
Irish J.C., Mills J.N., Turner-Ivey B., Wilson R.C., Guest S.T., Rutkovsky A., Dombkowski A., Kappler C.S., Hardiman G, & Ethier S.P. (2016). Amplification of WHSC1L1 regulates expression and estrogen‐independent activation of ERα in SUM‐44 breast cancer cells and is associated with ERα over‐expression in breast cancer. Molecular Oncology, 10(6), 850-865.
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