Abc total compensation beads

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom

The AbC Total Compensation beads are a set of beads designed for the quantification of antibodies. The beads are coated with specific antigens and can be used to measure the total concentration of antibodies in a sample.

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Lab products found in correlation

Efluor 780, by Thermo Fisher Scientific (2 mentions) Cd274 clone 29e 2a3, by BioLegend (2 mentions) Lmpo24, by Novus Biologicals (2 mentions) Attune nxt flow cytometer, by Thermo Fisher Scientific (2 mentions) Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (2 mentions) Arc amine reactive compensation beads, by Thermo Fisher Scientific (1 mentions) Countbright absolute counting beads, by Thermo Fisher Scientific (1 mentions) Lsrfortessa cell analyzer, by BD (1 mentions)

3 protocols using abc total compensation beads

Multiparametric Flow Cytometry Analysis

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All stained/fixed samples were acquired on Attune NxT Flow Cytometer (Thermo Fisher Scientific) with necessary internal controls to help assign gates. Fluorescence from multiple antibodies were compensated using AbC Total Compensation beads (Thermo Fisher Scientific, catalog no. A10497). In all experiments, cells were collected and stained with fixable viability dye eFluor780 (1:2000 dilution Life Technologies, catalog no. 65‐0865‐14;) followed by surface staining for PDL1 (CD274; clone 29E.2A3; BioLegend catalog no. 329714; 1:60 dilution) as per the manufacturer's instructions. Cells were then fixed with 4% methanol‐free formaldehyde (Thermo Fisher Scientific, catalog no. 28908) followed by intracellular staining for BZLF1 (Santa Cruz Biotechnology, catalog no. sc‐53904; 1:60 dilution) and LMP1 (clone LMPO24; Novus Biologicals, catalog no. NBP2‐50383; 1:60 dilution) using FoxP3/Transcription factor staining buffer set (eBioscience, catalog no. 5523) as per manufacturer's instructions. Data were analyzed using FlowJo and cumulated using GraphPad PRISM software.

Yan B., Wang C., Chakravorty S., Zhang Z., Kadadi S.D., Zhuang Y., Sirit I., Hu Y., Jung M., Sahoo S.S., Wang L., Shao K., Anderson N.L., Trujillo‐Ochoa J.L., Briggs S.D., Liu X., Olson M.R., Afzali B., Zhao B, & Kazemian M. (2022). A comprehensive single cell data analysis of lymphoblastoid cells reveals the role of super‐enhancers in maintaining EBV latency. Journal of Medical Virology, 95(1), e28362.

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Multiparametric Flow Cytometry of PDL1, BZLF1, and LMP1

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All stained/fixed samples were acquired on Attune NxT Flow Cytometer (Thermo Fisher Scientific) with necessary internal controls to help assign gates. Fluorescence from multiple antibodies were compensated using AbC Total Compensation beads (Thermo Fisher Scientific, catalog no. A10497). In all experiments, cells were collected and stained with fixable viability dye eFluor780 (1:2000 dilution Life Technologies, catalog no. 65–0865-14;) followed by surface staining for PDL1 (CD274; clone 29E.2A3; BioLegend catalog no. 329714; 1:60 dilution) as per the manufacturer’s instructions. Cells were then fixed with 4% methanol-free formaldehyde (Thermo Fisher Scientific, catalog no. 28908) followed by intracellular staining for BZLF1 (Santa Cruz Biotechnology, catalog no. sc-53904; 1:60 dilution) and LMP1 (clone LMPO24; Novus Biologicals, catalog no. NBP2–50383; 1:60 dilution) using FoxP3/Transcription factor staining buffer set (eBioscience, catalog no. 5523) as per manufacturer’s instructions. Data were analyzed using FlowJo and cumulated using GraphPad PRISM software.

Mair F, & Whitten P. (2000). Systematic review of studies of patient satisfaction with telemedicine. BMJ : British Medical Journal, 320(7248), 1517-1520.

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Comprehensive Lymphocyte Profiling by Flow Cytometry

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A 14-color, 17-parameter flow cytometry panel was used to enumerate different cell populations as described [26 (link)]. Briefly, fresh whole blood samples were stained for 30 min at room temperature with a panel of antibodies to lymphocyte surface markers (Supplementary Table S2) and 1:500 live/dead fixable near-infrared (near-IR) dye (Thermo Fisher Scientific, UK), followed by fixation and red blood cell lysis using red blood cell lysis/fixation solution (BioLegend, UK). After washing, CountBright™ absolute counting beads (Invitrogen, UK) were added to the sample and data was acquired using an LSR-Fortessa cell analyzer (BD Bioscience). Instrument calibration using cytometry setting and tracking (CST) beads, and compensation using AbC total compensation beads (Thermo Fisher Scientific, UK) and ArC Amine reactive compensation beads (Invitrogen, UK), were performed for each experiment.

Swieboda D., Rice T.F., Guo Y., Nadel S., Thwaites R.S., Openshaw P.J., Holder B, & Culley F.J. (2023). Natural killer cells and innate lymphoid cells but not NKT cells are mature in their cytokine production at birth. Clinical and Experimental Immunology, 215(1), 1-14.

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