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Anti rab5c

Manufactured by Abcam
Sourced in United States, United Kingdom

Anti-Rab5c is a primary antibody that recognizes the Rab5c protein. Rab5c is a member of the Rab family of small GTPases and is involved in the regulation of early endosome fusion and trafficking. The Anti-Rab5c antibody can be used for the detection and study of Rab5c in various applications, such as Western blotting and immunohistochemistry.

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2 protocols using anti rab5c

1

Antibody and Reagent Procurement for Cell Study

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Peroxidase-conjugated affinipure goat anti-rabbit IgG was purchased from Proteintech, United States. Rabbit monoclonal anti-LC3 antibody, anti-β-tubulin and anti-Rab5c were purchased from Abcam, United States. Hoechst 33342 and Rap were purchased from Sigma-Aldrich. The fluorescent label, Alexa Fluor 647 and 4% paraformaldehyde were purchased from Invitrogen. The lipophilic dye, DiO was purchased from Biotium, United States. The SGIV polyclonal anti-MCP antibody was prepared in our laboratory. The labeled si-EcRab5c sequence is listed in Supplementary Table 1, and was synthesized by GenePharma, China.
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2

Immunofluorescent Labeling of Organelles

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Anti‐Rab5C (Ab199530; Abcam, Cambridge, UK), Anti‐red, for detection of mCherry, (5F8 Chromotek, Munich, Germany), Anti‐ATP6V1G1 (16143‐1‐AP; Proteintech Europe, Manchester, UK), Anti‐EF2 (C14; Santa Cruz, Dallas), Anti Lamp‐1 (H4A3; Abcam), Alexafluor 568 anti‐rat (Thermo Fisher, Waltham). Horseradish peroxidase (HRP)‐conjugated secondary antibodies (Sigma Aldrich).
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