Anti mouse igg gb23301

Twenty-four BALB/c nude female mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. The mice were raised in the animal experimental center of of China Medical University. The twenty-four nude mice were randomly divided into four groups. Two groups were used for hypodermic injection, and the other two groups were used for intraperitoneally injected .In the subcutaneous tumorigenesis experiment, 3 × 106 SGC-7901 NC cells per mouse were injected in NC group,and 3*106 SGC-7901 sh-STEAP1 cells per mouse were injected in sh-STEAP1 group .Similarly, in the experiment of intraperitoneal tumorigenesis,3*106 SGC-7901 NC cells per mouse were injected in NC group,and 3 × 106 SGC-7901 sh-STEAP1 cells per mouse were injected in sh-STEAP1 group .The animals experiments primary antibodies were used as follows. STEAP1 (Ab207914,1:200) was purchased from Abcam. Ki-67(GB111141,1:500),C-CASPASE3(GB13436,1:200) were provided by Wuhan Servicebio technology Co., Ltd. IL1β(16806-1-AP, 1:200),IL6 (66146-1-AP, 1:200) were purchased from Proteintech Group, Wuhan, China. Secondary antibodies were used as follows, the goat anti-rabbit (GB23303, 1:200)and anti-mouse IgG (GB23301, 1:200) secondary antibodies were provided by Wuhan Servicebio technology Co., Ltd. All animal experimental steps were approved by the Animal Research Committee of China Medical University.