Mgcl2

Manufactured by Mallinckrodt

MgCl2 is a chemical compound composed of magnesium and chloride ions. It is a white, crystalline solid that is highly soluble in water and other polar solvents. MgCl2 is commonly used in various laboratory applications as a source of magnesium ions.

Automatically generated - may contain errors

Lab products found in correlation

Potassium chloride (kcl), by Mallinckrodt (3 mentions) 3 3 cholamidopropyl dimethylammonio 1 propanesulfonate chaps, by Merck Group (2 mentions) Adenosine 5 triphosphate atp, by Merck Group (2 mentions) Deoxycholic acid, by Merck Group (1 mentions) 5 bromo 4 chloro 3 indolyl β d galacto pyranoside x gal, by Thermo Fisher Scientific (1 mentions) K4fe cn 6, by Merck Group (1 mentions) Igepal, by Merck Group (1 mentions) K3fe cn 6, by Merck Group (1 mentions) Ethylenediaminetetraacetic acid edta, by Merck Group (1 mentions) Cacl2, by Merck Group (1 mentions) Nacl 7548 4400, by Daejung Chemicals (1 mentions) Hydrochloric acid (hcl), by Avantor (1 mentions) H2so4, by Avantor (1 mentions) D glucose, by Avantor (1 mentions) Citric acid, by Mallinckrodt (1 mentions) Hepes, by Mallinckrodt (1 mentions) Milli q, by Merck Group (1 mentions) Dithiothreitol (dtt), by Promega (1 mentions) Hydrochloric acid (hcl), by Mallinckrodt (1 mentions) Tris hcl ph 8, by Thermo Fisher Scientific (1 mentions)

6 protocols using mgcl2

Whole Cerebral Organoid X-gal Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole cerebral organoids were stored at −80 °C and, without thawing, fixed in 2% glutaraldehyde/2% formaldehyde in PBS for 10 min at room temperature. Organoids were incubated in ice-cold citrate phosphate buffer (CPB, pH 4.2) for 20 min, then incubated in X-gal working solution containing 40 mg/ml 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal, Invitrogen, 15520-018), 20 mM K₃Fe(CN)₆ (Sigma-Aldrich), 20 mM K₄Fe(CN)₆ (Sigma-Aldrich), 0.02% IGEPAL (Sigma-Aldrich), 0.01% deoxycholic acid (Sigma-Aldrich), and 2 mM MgCl₂ (Mallinckrodt) in CPB at 37 °C for 30 min to 2 h. The tissue was rinsed first with CPB and then with PBS, and imaged using a dissecting microscope.

Latour Y.L., Yoon R., Thomas S.E., Grant C., Li C., Sena-Esteves M., Allende M.L., Proia R.L, & Tifft C.J. (2019). Human GLB1 knockout cerebral organoids: A model system for testing AAV9-mediated GLB1 gene therapy for reducing GM1 ganglioside storage in GM1 gangliosidosis. Molecular Genetics and Metabolism Reports, 21, 100513.

+ Open protocol

+ Expand

Preparation of Biochemical Buffers and Solutions

Check if the same lab product or an alternative is used in the 5 most similar protocols

MgCl₂, KOH, KCl and 3-(N-morpholino)propanesulfonic acid (MOPS) were obtained from Mallinckrodt Baker (ACS reagents). NaOH and HCl were from Carlo Erba. RbCl, CaCl₂, adenosine 5′-triphosphate (ATP), 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) and ethylenediaminetetraacetic acid (EDTA) were obtained from Sigma Aldrich. RNAse-free ultrapure water (Arium mini Ultrapure Lab Water, Sartorius) was used to prepare all stock solutions and buffers. Concentrations of CaCl₂, MgCl₂ and EDTA in stock solutions were determined by volumetric titrations using a Zn²⁺ standard solution and eriochrome black T indicator²⁸.

Cababie L.A., Incicco J.J., González-Lebrero R.M., Roman E.A., Gebhard L.G., Gamarnik A.V, & Kaufman S.B. (2019). Thermodynamic study of the effect of ions on the interaction between dengue virus NS3 helicase and single stranded RNA. Scientific Reports, 9, 10569.

+ Open protocol

+ Expand

Comprehensive Intracellular and Extracellular Solutions

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following chemicals were used in the intracellular and extracellular solutions (for details, see Table 3 and 4, respectively): CsMeSO₃ (C1426), CsCl (289329), CsOH (C8518), NaCl (7548-4400, DAEJUNG), MgCl₂ (M9272), MgSO₄ (6070-12, Mallinckrodt Chemicals), sodium l-glutamate (49621), KCl (P5405), CaCl₂ (C7902), EGTA (E4378), MgATP (A9187), NaGTP (G8877), HEPES (H3375), citric acid (C2404), NaOH (7708-10, Mallinckrodt Chemicals), H₂SO₄ (339741), HCl (H1758), and d-glucose (1916-01, J.T. Baker). All chemicals were purchased from MilliporeSigma unless otherwise indicated.

Katz B., Zaguri R., Edvardson S., Maayan C., Elpeleg O., Lev S., Davidson E., Peters M., Kfir-Erenfeld S., Berger E., Ghazalin S., Binshtok A.M, & Minke B. (2023). Nociception and pain in humans lacking a functional TRPV1 channel. The Journal of Clinical Investigation, 133(3), e153558.

+ Open protocol

+ Expand

Quantification of Ionic Cofactors in Biochemical Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

KCl, KOH, HCl and MgCl₂ were from Mallinckrodt Baker (ACS reagents). 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES), ethylenediaminetetraacetic acid (EDTA) tetraacid form, 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), adenosine 5′-triphosphate (ATP) and adenosine 5′-(β,γ-imido)triphosphate (AMP-PNP) were from Sigma Aldrich. Dithiothreitol (DTT) was from Promega Corporation. All solutions were prepared in distilled and filtered water (MilliQ, Millipore Corporation) with no detectable RNase activity. Concentrations of EDTA and MgCl₂ in stock solutions were determined by volumetric titrations employing eriochrome black T indicator and a Zn²⁺ standard solution (22 ).

Gebhard L.G., Incicco J.J., Smal C., Gallo M., Gamarnik A.V, & Kaufman S.B. (2014). Monomeric nature of dengue virus NS3 helicase and thermodynamic analysis of the interaction with single-stranded RNA. Nucleic Acids Research, 42(18), 11668-11686.

+ Open protocol

+ Expand

Biomolecular Reagent Preparation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

MgCl2 (Mallinckrodt CHEMICALS, 6066-04), 100% Glycerol (Sigma-Aldrich, G5516), RNase/DNase-free UPW (Invitrogen, 10977-035), 1 M Tris–HCl pH 8.0 (Invitrogen, 15568–025), 1 M Tris–HCl pH 7.5 (Invitrogen, 15567-027), 0.5M EDTA (Invitrogen, AM9261), 1M DTT (Sigma-Aldrich, 43816), Tween-20 (Sigma-Aldrich, P1379), TBE ×10 (Fisher BioReagents, BP13334). Stock solutions of 4 M of NaCl (J.T. Baker, 0277), 3 M KCl (powder; MERCKGaA, 104936), 3 M LiCl (powder; J.T. Baker, 2370–01), 50mM ATP (Jena Bioscience, NU-1010) or 50 mM ATPgS (Jena Bioscience, NU-406) were prepared by dissolving the powders in RNase-free UPW or in UPW, treated with DEPC (Sigma-Aldrich, D5758) before use.

Danino Y.M., Molitor L., Rosenbaum-Cohen T., Kaiser S., Cohen Y., Porat Z., Marmor-Kollet H., Katina C., Savidor A., Rotkopf R., Ben-Isaac E., Golani O., Levin Y., Monchaud D., Hickson I.D, & Hornstein E. (2023). BLM helicase protein negatively regulates stress granule formation through unwinding RNA G-quadruplex structures. Nucleic Acids Research, 51(17), 9369-9384.

+ Open protocol

+ Expand

In Vitro Stock Reagents Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

We used the next stock reagents in this study, specifically for in vitro experiments: MgCl₂ (powder; Mallinckrodt CHEMICALS, 6066-04), 100% glycerol (Sigma-Aldrich, G5516), RNase/DNase-free UPW (Invitrogen, 10977-035), 1 M Tris–HCl pH 8.0 (Invitrogen, 15568-025), 1 M Tris–HCl pH 7.5 (Invitrogen, 15567-027), 0.5 M EDTA (Invitrogen, AM9261), 1 M DTT (Sigma-Aldrich, 43816), TBE ×10 (Fisher BioReagents, BP13334). Stock solutions of 3 M KCl (powder; MERCKGaA, 104936) or 3 M LiCl (powder; J.T. Baker, 2370-01), were prepared by dissolving the powders in UPW and treated with DEPC (Sigma-Aldrich, D5758) before use.

Turner M., Danino Y.M., Barshai M., Yacovzada N.S., Cohen Y., Olender T., Rotkopf R., Monchaud D., Hornstein E, & Orenstein Y. (2022). rG4detector, a novel RNA G-quadruplex predictor, uncovers their impact on stress granule formation. Nucleic Acids Research, 50(20), 11426-11441.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!