Room-temperature EvaGreen
fluorescence intensities for UV-exposed dsDNA solutions were measured
using a Safire fluorescence plate reader (Tecan, Mannendorf, Switzerland)
after the addition of EG dye followed by incubation at 37 °C
for 20 min in the dark. Fluorescence emission spectra were recorded
using an excitation wavelength of 490 nm and an emission wavelength
of 530 nm. The fluorescence spectra of dsDNA alone gave a minimum
or zero background at the 530 nm emission wavelength.
fluorescence intensities for UV-exposed dsDNA solutions were measured
using a Safire fluorescence plate reader (Tecan, Mannendorf, Switzerland)
after the addition of EG dye followed by incubation at 37 °C
for 20 min in the dark. Fluorescence emission spectra were recorded
using an excitation wavelength of 490 nm and an emission wavelength
of 530 nm. The fluorescence spectra of dsDNA alone gave a minimum
or zero background at the 530 nm emission wavelength.