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Phospho acc1 ser79

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Phospho-ACC1 Ser79 is a lab equipment product from Cell Signaling Technology that detects phosphorylation of Acetyl-CoA Carboxylase 1 (ACC1) at serine 79. This is a key regulatory site that controls the activity of ACC1, an enzyme involved in fatty acid synthesis.

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Lab products found in correlation

Phospho ampkα thr172, by Cell Signaling Technology (2 mentions) Ampkα, by Cell Signaling Technology (2 mentions) Raptor, by Cell Signaling Technology (2 mentions) Flag tag (flag), by Merck Group (2 mentions) Phospho gsk 3β ser9, by Cell Signaling Technology (1 mentions) Vinculin, by Cell Signaling Technology (1 mentions) P70s6k1, by Cell Signaling Technology (1 mentions) Phospho raptor ser792, by Cell Signaling Technology (1 mentions) Phospho p70s6k1 thr389, by Cell Signaling Technology (1 mentions) Tbc1d1, by Cell Signaling Technology (1 mentions) Phospho ulk1 ser555, by Cell Signaling Technology (1 mentions) Hsp90, by Cell Signaling Technology (1 mentions) Phospho akt thr308, by Cell Signaling Technology (1 mentions) Gsk3β, by Cell Signaling Technology (1 mentions) Phospho akt ser473, by Cell Signaling Technology (1 mentions) Phospho erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) α tubulin, by Merck Group (1 mentions) As160, by Merck Group (1 mentions) Ampk β1 2, by Cell Signaling Technology (1 mentions)

3 protocols using phospho acc1 ser79

1

Immunoblotting Analysis of Cellular Signaling

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AS160 (#07-741), Flag (#F7425), α-tubulin (#T6074), and phospho-TBC1D1 Ser237 (#07-2268) antibodies were purchased from MerckMilliporeSigma. ACC (#3676), phospho-ACC1 Ser79 (#3661), Akt (#4691), phospho-Akt Ser473 (#4060), phospho-Akt Thr308 (#9275) AMPKα (#2532), phospho-AMPKα Thr172 (#2535), phospho-AS160 Thr649 (#8881), ERK1/2 (#4695), phospho-ERK1/2 Thr202/Tyr204 (#4370), GSK3β (#9315), phospho-GSK3β Ser9 (#9322), HSP90 (#4874), p70S6K1 (#2708), phospho-p70S6K1 Thr389 (#9234), Raptor (#2280), phospho-Raptor Ser792 (#2083), TBC1D1 (#4629), ULK1 (#8054), phospho-ULK1 Ser555 (#5869), and vinculin (#13901) antibodies were purchased from Cell Signaling Technology.
Ahwazi D., Neopane K., Markby G.R., Kopietz F., Ovens A.J., Dall M., Hassing A.S., Gräsle P., Alshuweishi Y., Treebak J.T., Salt I.P., Göransson O., Zeqiraj E., Scott J.W, & Sakamoto K. (2021). Investigation of the specificity and mechanism of action of the ULK1/AMPK inhibitor SBI-0206965. Biochemical Journal, 478(15), 2977-2997.
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2

AMPK Pathway Protein Detection

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ACC (catalog no.: 3676), phospho-ACC1 (Ser79; catalog no.: 3661), AMPKα (catalog no.: 2532), phospho-AMPKα (Thr172; catalog no.: 2535), AMPKβ1 (catalog no.: 4178), Raptor (catalog no.: 2280), phospho-Raptor (S792; catalog no.: 2083), AMPKβ1 (catalog no.: 12063), AMPKβ2 (catalog no.: 4148), and AMPKβ1/2 (catalog no.: 4150) were obtained from Cell Signaling Technology. Antibody against AMPKγ1 (catalog no.: ab32508) was purchased from Abcam, and tubulin (catalog no.: T6074), FLAG (catalog no.: F7425), and β-actin (catalog no.: A2228) were purchased from MilliporeSigma. HTRF assay for detection of phospho-ACC was obtained from Cisbio (catalog no.: 64ACCPET).
Sanders M.J., Ratinaud Y., Neopane K., Bonhoure N., Day E.A., Ciclet O., Lassueur S., Naranjo Pinta M., Deak M., Brinon B., Christen S., Steinberg G.R., Barron D, & Sakamoto K. (2022). Natural (dihydro)phenanthrene plant compounds are direct activators of AMPK through its allosteric drug and metabolite–binding site. The Journal of Biological Chemistry, 298(5), 101852.
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3

Antibody Procurement and Western Blot

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Antibodies were purchased from Abcam (S-tag: GR124515-1), Sigma (Flag: F3040), Santa Cruz Biotechnology (Pin1: sc-271441, GAPDH: sc-47724, tubulin: sc-8035 and actin: sc-47778) or Cell Signaling Technology (ACC1: #4190, phospho-ACC1 (Ser79): #11818, ACC2: #8578, AMPK-alpha: #2532, and phosphor-AMPK-alpha (T172): #2535). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Nissui (Tokyo, Japan), SYBR Green from KAPA (Shiga, Japan).
Ueda K., Nakatsu Y., Yamamotoya T., Ono H., Inoue Y., Inoue M.K., Mizuno Y., Matsunaga Y., Kushiyama A., Sakoda H., Fujishiro M., Takahashi S.I., Matsubara A, & Asano T. (2019). Prolyl isomerase Pin1 binds to and stabilizes acetyl CoA carboxylase 1 protein, thereby supporting cancer cell proliferation. Oncotarget, 10(17), 1637-1648.
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