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Phospho acc1 ser79

Manufactured by Cell Signaling Technology

Phospho-ACC1 Ser79 is a lab equipment product from Cell Signaling Technology that detects phosphorylation of Acetyl-CoA Carboxylase 1 (ACC1) at serine 79. This is a key regulatory site that controls the activity of ACC1, an enzyme involved in fatty acid synthesis.

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3 protocols using phospho acc1 ser79

1

Immunoblotting Analysis of Cellular Signaling

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AS160 (#07-741), Flag (#F7425), α-tubulin (#T6074), and phospho-TBC1D1 Ser237 (#07-2268) antibodies were purchased from MerckMilliporeSigma. ACC (#3676), phospho-ACC1 Ser79 (#3661), Akt (#4691), phospho-Akt Ser473 (#4060), phospho-Akt Thr308 (#9275) AMPKα (#2532), phospho-AMPKα Thr172 (#2535), phospho-AS160 Thr649 (#8881), ERK1/2 (#4695), phospho-ERK1/2 Thr202/Tyr204 (#4370), GSK3β (#9315), phospho-GSK3β Ser9 (#9322), HSP90 (#4874), p70S6K1 (#2708), phospho-p70S6K1 Thr389 (#9234), Raptor (#2280), phospho-Raptor Ser792 (#2083), TBC1D1 (#4629), ULK1 (#8054), phospho-ULK1 Ser555 (#5869), and vinculin (#13901) antibodies were purchased from Cell Signaling Technology.
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2

AMPK Pathway Protein Detection

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ACC (catalog no.: 3676), phospho-ACC1 (Ser79; catalog no.: 3661), AMPKα (catalog no.: 2532), phospho-AMPKα (Thr172; catalog no.: 2535), AMPKβ1 (catalog no.: 4178), Raptor (catalog no.: 2280), phospho-Raptor (S792; catalog no.: 2083), AMPKβ1 (catalog no.: 12063), AMPKβ2 (catalog no.: 4148), and AMPKβ1/2 (catalog no.: 4150) were obtained from Cell Signaling Technology. Antibody against AMPKγ1 (catalog no.: ab32508) was purchased from Abcam, and tubulin (catalog no.: T6074), FLAG (catalog no.: F7425), and β-actin (catalog no.: A2228) were purchased from MilliporeSigma. HTRF assay for detection of phospho-ACC was obtained from Cisbio (catalog no.: 64ACCPET).
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3

Antibody Procurement and Western Blot

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Antibodies were purchased from Abcam (S-tag: GR124515-1), Sigma (Flag: F3040), Santa Cruz Biotechnology (Pin1: sc-271441, GAPDH: sc-47724, tubulin: sc-8035 and actin: sc-47778) or Cell Signaling Technology (ACC1: #4190, phospho-ACC1 (Ser79): #11818, ACC2: #8578, AMPK-alpha: #2532, and phosphor-AMPK-alpha (T172): #2535). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Nissui (Tokyo, Japan), SYBR Green from KAPA (Shiga, Japan).
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