Tracegold tg 5silms

The absorbances of the samples were obtained using a Perkin Elmer Life and Analytical Science Molecular Absorption Spectrophotometer LAMBDA 25, at a λ = 595 nm. For the total nitrogen determination by the Kjeldahl method, a Digesdahl 23130–21 digestion apparatus was used. A Thermo Scientific TSQ 8000 EVO gas chromatograph was used under the following conditions: Column, Thermo Scientific TraceGOLD TG-5SilMS, L = 30.00 m, D = 0.25 mm; The used carrier gas, He; Flow rate, 1 mL/min; Analysis time, 46.667 min; Detection with Total Ion Chromatogram (ICT), 40–600 M/z; Software, Chromeleon 7. Protein analysis by polyacrylamide gel electrophoresis was performed using the Bio-Rad PowerPac Basic Mini electrophoresis system. A Perkin Elmer Life and Analytical Science LAMBDA 25 molecular absorption spectrophotometer was used for dosing of fructan fibers at 520 nm.

A Thermo Scientific TRACE 1310 Gas Chromatography coupled with TSQ 8000 Evo Triple Quadrupole Detector and AI 1310 Auto Sampler was used with Thermo Xcalibur 2.2 mass spectrometry data system (Software). For the chromatographic separation, a Thermo Scientific™ Trace GOLD™ TG-5SilMS 30 m × 0.25 mm I.D. × 0.25 µm film capillary column was used. The flow rate was maintained at constant flow 1.2 mL/min (He, inert carrier gas). The GC oven program was initially set at 70 °C (2 min), then increased 25 °C/min to 180 °C, 5 °C/min to 200 °C, and 10 °C/min to 280 °C, kept constant 5 min. The MS was operated with electrospray ionization (ESI+). The optimized parameters included transfer line (280 °C), electron energy(eV) 70, acquisition mode (SRM), and ion source temperature (320 °C). The Thermo ScientificTM TraceFinderTM software was used for method setup and data processing. For all pesticide compounds two SRM transitions were chosen for the overall MRM acquisition method. The first transition was used for quantitation, the second transition for confirmation. Table 5 and Figure 9, lists the SRM parameters for the compounds analyzed in this method.

Digital microscope, DinoLight (AnMo Electronic Corp. Hsinchu, Taiwan); 5-digit anti-static scale (METTLER TOLEDO, XSR225 DualRange, Switzerland); high temperature laboratory oven LHT 6/60 (Carbolite Gero, Sheffield, UK); Nicolet™ Summit PRO FTIR spectrometer equipped with an Everest™ ATR accessory monolithic ATR crystal (ThermoFisher Scientific™, Waltham, MA, USA); Frontier Lab Multi-Shot Pyrolyzer™ (EGA/PY-3030D) with Auto-Shot Sampler™ (AS-1020E; Frontier Lab, Koriyama, Japan), coupled to a Thermo Scientific™ TRACE™ 1310 Gas Chromatograph with a Thermo Scientific™ TraceGOLD™ TG-5SilMS 30 m × 0.25 mm I.D. × 0.25 μm film capillary column (P/N 26096-1420; ThermoFisher Scientific™, Waltham, MA, USA), coupled to a Thermo Scientific™ Exactive™ GC Orbitrap™ mass spectrometer.