Nε acetyl l lysine

Manufactured by Merck Group

Nε-Acetyl-l-lysine is a chemical compound used as a lab equipment product. It is a derivative of the amino acid lysine, with an acetyl group attached to the epsilon nitrogen. This compound is commonly used in various research and analytical applications, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach.

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Close spelling variants of this product

L-lysine (207 citations) Acetyl-lysine (12 citations) Nε-acetyl-l-lysine (AcK, (2 citations)

3 protocols using nε acetyl l lysine

Acetylation of Lysine in HEK293E Cells

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HEK293E cells were plated and cultured in DMEM with 10% FBS and transfected with FuGENE6 (Promega) 24 h later. Twenty four or 48 h after transfection the media was substituted with DMEM with 10%FBS and 5 mM Nε-Acetyl-l-lysine (A4021, Sigma). Cells were lysed or fixed after being 24 h in the presence of acetylated lysine. When mentioned, Ex527 (CAS 49843-98-3, Santa Cruz Biotechnology) was dissolved in DMSO and added to the cultured cells 24 h before lysis to a final concentration of 1 or 10 µM.

Garcia Morato J., Hans F., von Zweydorf F., Feederle R., Elsässer S.J., Skodras A.A., Gloeckner C.J., Buratti E., Neumann M, & Kahle P.J. (2022). Sirtuin-1 sensitive lysine-136 acetylation drives phase separation and pathological aggregation of TDP-43. Nature Communications, 13, 1223.

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Enzymatic Characterization of Ribonuclease A

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Pancreatic ribonuclease A was purchased from Worthington Biochemical Corparation. L-Lysine, Nα-Acetyl-L-Lysine, NεAcetyl-L-Lysine, DLMethionine, NAcetyl-DL-Methionine, Histidine, NαAcetyl-DL-Histidine, Imidazole, NαAcetyl-L-Arginine, NαAcetyl-L-Glutamine, NαAcetyl-DL-Tryptophan, NαAcetyl-DLTyrosine, Ribonucleic acid, Chloramine T trihydrate, Potassium Bromide, Lanthanum nitrate hydrate, Sodium Iodide, 3,3′,5,5′-Tetramethylbenzidine (TMB), N,N-Dimethyl formamide (DMF), Pyridoxamine dihydrochloride, Guanidinium Hydrochloride, Sodium Hypochlorite solution, proteinase K and type IV collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane were purchased from Sigma-Aldrich. Human Integrin α1β1 was purchased from Millipore and β1 Integrin antibody was purchased from Santa Cruz Biotechnology Inc.

Madu H., Avance J., Chetyrkin S., Darris C., Rose K.L., Sanchez O.A., Hudson B, & Voziyan P. (2015). Pyridoxamine Protects Proteins from Damage by Hypohalous Acids in Vitro and in Vivo. Free radical biology & medicine, 89, 83-90.

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Antibody-Based Protein Analysis Protocol

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Anti-Sirt1 (#2493, 1:1000), anti-LKB1 (#13031, 1:1000), anti-acetyl-p53 K382 (#2525, 1:1000), anti-p53 (#18032, 1:1000), anti-phospho-AMPK T172 (#2535, 1:1000), anti-AMPK (#5831, 1:1000), anti-phospho-ACC Ser79 (#11818, 1:1000), anti-ACC (#3662, 1:1000), anti-MO25α (#2716, 1:2000), anti-acetylated-lysine (#9441, 1:1000), anti-Lamin B1 (#13435, 1:1000), anti-GST-tag (#2625, 1:2000), anti-His-tag (#12698, 1:2000), anti-HA-tag (#3724, 1:2000), and anti-MBP-tag (#2396, 1:2000) antibodies were from Cell Signaling Technology. Anti-phospho-Ser/Thr (ab17464, 1:1000) and anti-α-tubulin (ab80779, 1:5000) antibodies were from Abcam. Anti-STRAD (N-13) (sc-34102) was from Santa Cruz. Anti-FLAG-tag (M2, 1:2000) antibody was from Sigma. Lambda Protein Phosphatase (P0753) was from New England Biolabs. Resveratrol, piceatannol, quercetin, fisetin, EX527, nicotinamide, NAD, N-ε-acetyl-L-lysine, SRT1720, 3xFLAG peptide, doxorubicin, phosphatase inhibitors, and protease inhibitors were from Sigma.

Huang Y., Lu J., Zhan L., Wang M., Shi R., Yuan X., Gao X., Liu X., Zang J., Liu W, & Yao X. (2021). Resveratrol-induced Sirt1 phosphorylation by LKB1 mediates mitochondrial metabolism. The Journal of Biological Chemistry, 297(2), 100929.

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