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Propan 2 ol

Manufactured by Scharlab
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Sourced in Spain

Propan-2-ol, also known as isopropyl alcohol, is a colorless, flammable liquid chemical. It has the chemical formula C3H8O. Propan-2-ol is commonly used as a solvent and disinfectant in various laboratory applications.

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Lab products found in correlation

Sodium hydroxide (naoh), by Merck Group (3 mentions) Ammonium acetate, by Merck Group (2 mentions) Formic acid, by Merck Group (2 mentions) Acetic acid, by Merck Group (2 mentions) Ammonium hydroxide, by Merck Group (2 mentions) Ammonium bicarbonate, by Merck Group (2 mentions) Sodium citrate, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Iodoacetamide, by Merck Group (1 mentions) Glu c, by Promega (1 mentions) Dl dithiothreitol, by Merck Group (1 mentions) Ammonium hydrogen carbonate, by Merck Group (1 mentions) Esi low concentration esi l tuning mix, by Agilent Technologies (1 mentions) Trypsin, by Promega (1 mentions) Orthophosphoric acid, by Merck Group (1 mentions) Glacial acetic acid, by Merck Group (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Chloroform, by Merck Group (1 mentions) Guanidinium chloride, by Merck Group (1 mentions) Acetonitrile, by ITW Reagents (1 mentions)

3 protocols using propan 2 ol

1

Optimized Protein Extraction and Digestion

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All buffers and solutions
were prepared with analytical reagent grade chemicals. Acetic acid
(HAc, glacial), formic acid (HFor 98–100%), sodium hydroxide,
sodium citrate tribasic, and ammonia (25%) were provided by Merck
(Darmstadt, Germany). DL-Dithiothreitol (DTT, ≥99%), iodoacetamide
(IAA, ≥98%), ammonium acetate (≥99.9%), and ammonium
hydrogen carbonate (≥99.9%) were supplied by Sigma-Aldrich
(St. Louis, MO, USA). Propan-2-ol was purchased from Scharlab (Barcelona,
Spain), while acetonitrile and water were supplied by Sigma-Aldrich
(all of them were of LC-MS quality grade). Trypsin and Glu-C (both
sequencing grade modified) were provided by Promega (Madison, WI,
USA). The ESI low concentration (ESI-L) tuning mix for tuning and
calibration of the mass spectrometer was obtained from Agilent Technologies
(Waldbronn, Germany).
Mancera-Arteu M., Benavente F., Sanz-Nebot V, & Giménez E. (2023). Sensitive Analysis of Recombinant Human Erythropoietin Glycopeptides by On-Line Phenylboronic Acid Solid-Phase Extraction Capillary Electrophoresis Mass Spectrometry. Journal of Proteome Research, 22(3), 826-836.
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2

Immobilization of DNA Aptamer on Magnetic Beads

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All chemicals used in the preparation
of the BGE and the rest of solutions were of analytical reagent grade
or better. Acetic acid (HAc, glacial), ammonium hydroxide (NH4OH) (25%), formic acid (HFor, 99.0%), and sodium hydroxide
(≥99.0%) were supplied by Merck (Darmstadt, Germany). Ammonium
bicarbonate (LC-MS grade) was purchased from Sigma-Aldrich (St. Louis,
MO). Propan-2-ol (LC-MS) was purchased from Scharlau (Barcelona, Spain).
Water (LC-MS grade) was supplied by Fisher Scientific (Loughborough,
U.K.). Particles with immobilized trypsin were provided by Promega
(Madison, WI).
The DNA aptamer M5-1530 (link) modified with a C6 spacer arm terminated by 5′amino (M5-15-5′,
66-mer, Mr = 20 690) and purified
by HPLC, was purchased from Integrated DNA Technologies (Coralville,
IA). LOABeads AffiAmino magnetic beads (MBs) of 45–165 μm
diameter were purchased from Lab on a Bead (Uppsala, Sweden).
Salim H., Pero-Gascon R., Giménez E, & Benavente F. (2022). On-line Coupling of Aptamer Affinity Solid-Phase Extraction and Immobilized Enzyme Microreactor Capillary Electrophoresis-Mass Spectrometry for the Sensitive Targeted Bottom-Up Analysis of Protein Biomarkers. Analytical Chemistry, 94(19), 6948-6956.
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3

Preparation and Characterization of miRNA Solutions

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All the chemicals used in the preparation of background electrolytes (BGEs) and solutions were of analytical-reagent grade or better. Acetic acid (glacial), ammonium hydroxide (25%), guanidinium chloride (≥99.0%), ortho-phosphoric acid (85%), and sodium hydroxide (≥99.0%) were purchased from Merck (Darmstadt, Germany).
Acetonitrile (LC-MS), 96% ethanol and methanol (LC-MS) were supplied by Panreac AppliChem (Barcelona, Spain). Propan-2-ol (LC-MS) was purchased from Scharlau (Barcelona, Spain). Ammonium acetate (NH4Ac, ≥99.999%), ammonium bicarbonate (for LC-MS), chloroform (≥99.0%), and phenol/chloroform/isoamyl alcohol mixture (PCA, 25:24:1) were supplied by Sigma-Aldrich (Madrid, Spain). The TRIzol Reagent was purchased from Thermo Fisher Scientific (Barcelona, Spain). Synthetic miRNA hsa-miR-21-5p (miR-21-5p), hsa-let-7g-5p (let-7g-5p), and hsa-miR-16-5p with 3'uridylation (iso-miR-16-5p) were purchased from IDT (Coralville, IA, USA).
Pero-Gascon R., Sanz-Nebot V., Berezovski M.V, & Benavente F. (2018). Analysis of Circulating microRNAs and Their Post-Transcriptional Modifications in Cancer Serum by On-Line Solid-Phase Extraction-Capillary Electrophoresis-Mass Spectrometry. Analytical chemistry, 90(11).
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