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Ni usb 6363

Manufactured by National Instruments
Sourced in Japan

The NI USB-6363 is a data acquisition (DAQ) device that provides high-speed analog input, analog output, digital input/output, and counter/timer functionality. It is designed to interface with a variety of sensors and signals for laboratory and test and measurement applications.

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6 protocols using ni usb 6363

1

Aorta Pressure-Diameter Characterization

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The pressure–diameter test was performed based on the previous study (Sugita and Matsumoto 2017 (link)). Both proximal and distal sides of the tubular aorta were tied to a 22-G hypodermic needle with suture threads so that its ventral side was top through the specimen as reference to the gentian violet markers. The aorta was stretched to its in vivo length with reference to the gentian violet markers. The specimen was pressurized with an electro-pneumatic regulator (640BA20B, Asahi Enterprise, Tokyo, Japan). The regulator was controlled using a software (NI LabVIEW 2010, National Instruments, Austin, TX, USA) installed on a personal computer (FMV BIBLO, Fujitsu, Tokyo, Japan; PC) through a digital–analog (D/A) converter (NI USB-6363, National Instruments). The intraluminal pressure of the specimen was measured with a pressure transducer (DX-300, Nihon Kohden, Tokyo, Japan), a strain amplifier (DPM-911B, Kyowa Electronic Instruments, Chofu, Japan), an analog–digital (A/D) converter (NI USB-6363, National Instruments), and the PC.
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2

Comparison of N-FSCV and N-FCSWV Techniques

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Conventional N-FSCV was used to make a comparison with N-FCSWV.8 (link) N-FSCV applied with a 1000 V/s scan rate and 0.2 V holding potential versus Ag/AgCl. The waveform switching potential was 1.0 V and then to −0.1 V and back to 0.2 V holding potential.
N-FSCV was performed using WINCS Harmoni with WincsWare software,21 (link) and N-FCSWV were achieved using a data acquisition interface (NI USB-6363, specification, National Instruments) with in-house written software using LabView (National Instruments, Austin, TX) to operate on a base-station computer. A current to voltage preamplifier was used with no analog filter circuit in order to maintain the immediate current response to the square pulse in N-FCSWV. An in-house program with the NI USB-6363 interface controlled the application of the waveform and flow injection hardware. Data was stored on the base-station computer in the form of a sequence of unsigned 2-byte integers and processed by MATLAB (MathWork Inc., Natick, MA). Temporal averaging, filtering, and background currents simulation were applied during post signal processing. Unlike conventional square wave voltammetry, all data points were recorded and used to analyze including curve fitting.12 (link) Figures and statistics (one-way, two-way ANOVA with multiple comparisons, paired t test) were created by using GraphPad Prism 5 (GraphPad Software, San Diego, CA).
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3

Fiber-Coupled Laser Cantilever Force Measurement

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A schematic of our force-measurement setup is given in Fig. 2a. A Sheaumann HF-975-7500-25C fibre-coupled laser diode, powered by an Arroyo LaserSource controller, was used for actuating the system. The vial or spectroscopic cuvette were glued to a 3 cm-long metal cantilever (made from a metal ruler). A small mirror was glued to the other side of the cantilever. A low power red laser was reflected on the small mirror and directed onto a quadrant photodiode (Thorlabs, PDQ80A). Laser illumination above the threshold power (see Fig. 2c) resulted in force spikes, which induced deflection of the cantilever in the 10 μm range. The resolution in cantilever deflection was below 100 nm and the time resolution was given by the photodiode bandwidth (150 kHz). In all experiments, the excitation laser fibre tip was placed at exactly 1 mm from the vial wall using a stepper motor and 2 mm above the vial bottom. The analogue control of the laser power, camera triggers, and force and sound measurements were synchronised using a National Instruments DAQ card (NI USB-6363) and a custom Labview software.
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4

Multichannel Cyclic Voltammetry Analysis

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M-CSWV was performed using a commercial electronic interface (NI USB-6363, National Instruments) with a base-station PC and software written in-house using LabVIEW 2016 (National Instruments, Austin, TX). Data, in the form of a sequence of unsigned 2-byte integers, were saved to the base-station computer and processed by MATLAB (MathWork Inc., Natick, MA). The processing includes temporal averaging, filtering, and simulating background currents. GraphPad Prism 5 (GraphPad Software, San Diego, CA) was used to generate figures and perform statistics (one-way, two-way ANOVA with multiple comparisons, etc.). All data are presented as mean ± standard error of the mean (SEM) values for n number of electrodes or rats.
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5

Crayfish Walking Leg Neurophysiology

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Data acquisition and analysis were performed with Igor Pro (WaveMetrics). Voltage signals were filtered at 5 kHz and sampled at 50 kHz (NI USB-6363). Each preparation ( N ) represented a set of data recorded from the walking leg of a crayfish animal. Statistical results were presented as an average±the standard error of the mean (SEM). Samples with statistically significant differences were tested with the two-tailed Student’s t -test ( α=0.05 ).
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6

Femtosecond Two-Photon Laser Scanning Microscopy

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The schematic setup for the LSM is shown in Fig. 1. A tunable femtosecond Ti:sapphire laser (720-950 nm, 80 MHz repetition rate, Chameleon, Coherent Inc., Santa Clara, California) provided excitation light. A polarizing beamsplitter cube deflected confocal reflectance light to the avalanche photodiode (APD) detector. A long-pass dichroic mirror (DM) directed the twophoton excited fluorescence (TPEF) signal to a photomultiplier tube (PMT). The microscopic objective (MO) (Olympus America Inc., Center Valley, Pennsylvania) was a ×40 water immersion type. The optical scanner was a small beam diameter scanning galvanometer mirrors system (GVS202, Thorlabs, Newton, New Jersey). The motor had a maximum angular scanning range of 12.5 deg, a repeatability of 15 μrad, and a step response time of 300 μs. Within a small scan angle (AE0.2 deg), the maximum input frequency could go up to 1 KHz. The galvo driving board accepted external command input from a DAQ card. The driving board provided diagnostic pins with voltage signals proportional to the position of the scanner mirror. A multifunction DAQ card (NI USB-6363, National Instruments, Austin, Texas) drove the galvo, acquired APD and PMT output, and detected the galvo mirror position diagnostic signals.
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