After rats were anesthetized, a midline abdominal incision was made, and a cotton swab was used to open the renal adipose capsule. Saline-soaked sterile gauze was used to cover the intestines. The abdominal aorta and renal artery were separated, with the renal artery exposed at about 3 cm from the abdominal aorta. All visible renal nerve bundles were removed and adventitia of the renal artery and vein were stripped. Blood vessels were then gently painted with a cotton swab soaked with a solution of 95% ethanol and 10% phenol in ethanol. In selective rats, we evaluated the effects of RD by electrically stimulating the renal sympathetic nerves (Grass S48 nerve stimulator, 15 V, 0.2 ms, 10 Hz) at the proximal renal artery for 10–30 seconds before and after RD. In normal rats, electrical stimulation increased blood pressure by 5–10 mmHg, increased heart rate by 8–15 bpm, and caused the kidney to become paler in color. When electrically stimulated after RD, sympathetic effects were absent with no apparent changes in heart rate, blood pressure, or kidney color.
S48 nerve stimulator
Manufactured by Natus
The S48 nerve stimulator is a medical device designed to electrically stimulate nerves. It generates electrical pulses that can be applied to the patient's body to elicit a nerve response, which can be useful in various clinical applications.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using s48 nerve stimulator
1
Renal Denervation Procedure in Rats
2
Surgical Renal Denervation in Myocardial Infarction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Two days after induction of myocardial infarction, bilateral RD was performed under intraperitoneal administration of a mixture of ketamine (50 mg/kg) and diazepam (5 mg/kg). Bilateral flank incisions were made, and RD was performed surgically by stripping the adventitia of the renal arteries at about 3 mm from the abdominal aorta for 1–2 cm and dissecting all visible renal nerve bundles, and coating the vessels with a solution of 10% phenol in ethanol. We evaluated the effects of RD by electrically stimulating (Grass S48 nerve stimulator, 15 V, 0.2 ms, 10 Hz) the renal sympathetic nerve at the proximal renal artery for 10–30 seconds before and after RD, respectively. In normal rats, electrical stimulation can cause the blood pressure to increase 5-10 mmHg, the heart rate (HR) to increase 8-15 bpm, and the kidney to become pale in color. When electrically stimulated after RD, sympathetic effects were absent with no apparent changes in blood pressure, HR heart rate, or kidney color.
3
Renal Sympathetic Denervation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RSD was performed under intraperitoneal anesthesia (40 mg/kg pentobarbital sodium). Both kidneys were surgically denervated by cutting all visible nerves from the renal arteries and veins under an operating microscope. The vessels were then coated with a 20% phenol/ethanol solution for 10–15 minutes. The renal sympathetic nerves were electrically stimulated for 20–30 seconds to evaluate the effects of RSD (Grass S48 nerve stimulator, 0.2 ms, 15 V, 10 Hz). In normal rats, the blood pressure increased by 5–10 mmHg, heart rate increased by 8–15 bpm, and the kidney became paler after electrical stimulation. Changes in blood pressure, heart rate, or kidney color were absent in animals after RSD.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!