3 4 dihydroxyphenylacetic acid

Manufactured by Merck Group

Sourced in United States, Spain, Germany

3,4-dihydroxyphenylacetic acid is a chemical compound. It is a colorless crystalline solid. The compound has the molecular formula C₈H₈O₃.

Automatically generated - may contain errors

Lab products found in correlation

32 protocols using 3 4 dihydroxyphenylacetic acid

Quantification of Polyphenolic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

US Pharmacopeia reference standard compounds, including trans-RSV, (+)-catechin, (–)-epicatechin, gallic acid, 3-hydroxytyrosol, isochlorogenic acid, 3, 4-dihydroxy phenylacetic acid, 4-methyl gallic acid, 3, 4-dihydroxy phenylacetic acid, 3-hydroxybenzoic acid, caffeic acid, 4-hydroxybenzoic acid, vanillic acid, dihydromyricetin, syringic acid, resveratrol-3-glucoside, dihydro ferulic acid, sinapic acid, taxifolin, ferulic acid, 3-hydrocinnamic acid, phenylacetic acid, and trans-2-hydrocinnamic acid, were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Primary analytical standard (Grade P) compounds, including procyanidin B2, procyanidin C1, quercetin, and cyanidin-3-glucoside, were purchased from ChromaDex (Irvine, CA, USA). The HPLC grade water, acetonitrile (ACN), methanol (MeOH), formic acid (FA), and trifluoroacetic acid (TFA) were obtained from Fisher Scientific Co. (Fair Lawn, NJ, USA).

Lyu W., Rodriguez D., Ferruzzi M.G., Pasinetti G.M., Murrough J.W., Simon J.E, & Wu Q. (2021). Chemical, Manufacturing, and Standardization Controls of Grape Polyphenol Dietary Supplements in Support of a Clinical Study: Mass Uniformity, Polyphenol Dosage, and Profiles. Frontiers in Nutrition, 8, 780226.

+ Open protocol

+ Expand

HPLC Analysis of Neurotransmitters

Check if the same lab product or an alternative is used in the 5 most similar protocols

Disodium EDTA, sodium phosphate, o-phosphoric acid (85%, HPLC grade), acetonitrile (Optima LC/MS grade), acetone (HPLC grade), and 2-propanol (99.9 %, HPLC grade) were purchased from Fisher Scientific (Fair Lawn, NJ). Sodium acetate and glacial acetic acid (99.9 %) were from J.T. Baker (Phillipsburg, NJ). Sodium 1-octanesulfonate (SOS), L-ascorbic acid (AA), 3,4 dihydroxyphenylacetic acid (DOPAC), 5-hydroxyindole-3-acetic acid (5-HIAA), homovanillic acid (HVA), dopamine hydrochloride (DA), serotonin hydrochloride (5-HT), 3-methoxytyramine hydrochloride (3-MT) were from Sigma Aldrich (St. Louis, MO). Nomifensine maleate (Sigma) was dissolved in phosphate-buffered saline (PBS: 155 mM NaCl, 100 mM NaH₂PO₄, pH 7.40) and administered at 20 mg/kg (i.p.). Artificial cerebrospinal fluid (aCSF) was 144 mM NaCl, 2.7 mM KCl, 1.1.75 mM CaCl₂, 1 mM MgCl₂, 1.75 mM NaH₂PO₄ and 2.5 mM NaHCO₃. All chemicals were used as received without further purification. Ultrapure water was obtained from a Millipore Milli-Q Synthesis A10 system (Bedford, MA).

Gu H., Varner E.L., Groskreutz S.R., Michael A.C, & Weber S.G. (2015). In vivo Monitoring of Dopamine by Microdialysis with One-Minute Temporal Resolution Using Online Capillary Liquid Chromatography with Electrochemical Detection. Analytical chemistry, 87(12), 6088-6094.

+ Open protocol

+ Expand

Synthesis and Preparation of Phenolic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tyrosine, 3-4-hydroxy-phenyl-propionic acid (HPA), 3,4-dihydrobenzaldehyde (DHB), 3,4-dihyroxybenzoic acid (DHBA), 3,4 dihydroxymandelic acid (DHMA), L-3,4-dihydroxyphenylalanine (DOPA) and 3,4-dihydroxyphenylacetic acid (DOPAC), 3,4-hydroxyphenyl lactic acid (HPLA), 2,4-Dihydroxyphenyl propionic acid (DHPA) and 4-hydroxyphenylpyruvic acid (HPPA) were purchased from Sigma-Aldrich; dihydrocaffeic acid (DHCA), 3,4-dihydroxy-phenyl pyruvate (DPPA) and 3-4-dihydroxyphenyl lactic acid (DPLA) were obtained from Extrasynthese, Molekula and Stanford Chemicals respectively. With the exception of DOPA, stock solutions at 1M were prepared in 70% ethanol, aliquoted and stored at −20°C. For subsequent dilution to a final concentration below 20 mM, fresh aliquots were first diluted to 50 mM in deionized water and then to their final concentration in 50 mM NaCl. For DOPA, a 10 mM stock solution was prepared in 50 mM NaCl, aliquoted and stored at −20°C. Fresh aliquots were diluted to their final concentration in 50 mM NaCl.

Zugasti O., Bose N., Squiban B., Belougne J., Kurz C.L., Schroeder F.C., Pujol N, & Ewbank J.J. (2014). Activation of a G protein-coupled receptor by its endogenous ligand triggers the Caenorhabditis elegans innate immune response. Nature immunology, 15(9), 833-838.

+ Open protocol

+ Expand

Quantitative Analysis of Neurotransmitters

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), 3-methoxytyramine (3-MT), noradrenaline, 3-methoxy-4-hydroxyphenylethyleneglycol (MHPG), normetanephrine (NM), and l-Tyr were obtained from Sigma-Aldrich (St. Louis, MO, USA). The dipeptides IY, SY, and Tyr-Pro (YP) were synthesized using a 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase synthesis method according to the manufacturer's instructions (Kokusan Chemical Co., Osaka, Japan) [9 (link)].

Moriyasu K., Ichinose T., Nakahata A., Tanaka M., Matsui T, & Furuya S. (2016). The Dipeptides Ile-Tyr and Ser-Tyr Exert Distinct Effects on Catecholamine Metabolism in the Mouse Brainstem. International Journal of Peptides, 2016, 6020786.

+ Open protocol

+ Expand

Analytical Standards for Phenolic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Acetonitrile of HPLC grade, acetic acid and N,O-bis(trimethylsilyl)trifluoroacetamide + 10% trimethylchlorosilane (BSTFA + 10% TMCS) were from Fischer Scientific (Pittsburgh, PA, USA). 5-O-caffeoylquinic acid, (+)-catechin, (−)-epicatechin, sucrose, glucose, fructose, citric acid, NaBH₄, N-methylimidazole, acetic anhydride, benzoic acid, 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, 3,4-dihydroxybenzoic acid, 3-phenylpropionic acid, phenylacetic acid, 3-hydroxyphenylacetic acid, 3-(4-hydroxyphenyl)propionic acid, dl-3-phenyllactic acid, dl-3-(4-hydroxyphenyl)lactic acid, 5-phenylvaleric acid, 3,4-dihydroxyphenylacetic acid and 2,4,5-trimethoxycinnamic acid and toluene-α−thiol were obtained from Sigma-Aldrich (Saint Quentin Fallavier, France). Phloretin, p-coumaric acid, quercetin and cyanidin-3-O-galactoside were obtained from Extrasynthese (Lyon, France). Phloridzin was obtained from Fluka (Buchs, Switzerland). Malic acid was obtained from R-Biopharm (Darmstadt, Germany). D3-Methanol was from Acros Organics (Geel, Belgium). Acetonitrile was analytical grade and from Fisher Scientific (Fair Lawn, NJ, USA). Ethyl acetate, dichloromethane and hexane obtained from VWR International (Radnor, PA, USA).

Le Bourvellec C., Bagano Vilas Boas P., Lepercq P., Comtet-Marre S., Auffret P., Ruiz P., Bott R., Renard C.M., Dufour C., Chatel J.M, & Mosoni P. (2019). Procyanidin—Cell Wall Interactions within Apple Matrices Decrease the Metabolization of Procyanidins by the Human Gut Microbiota and the Anti-Inflammatory Effect of the Resulting Microbial Metabolome In Vitro. Nutrients, 11(3), 664.

+ Open protocol

+ Expand

Synthesis and Preparation of Phenolic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

Camellia Species Seed Maturation Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

A total of 40 Camellia spp. samples in seed maturation were provided by Prof. Kaibing Zhou in 2018 for this study, including 26 C. vietnamensis Huang varieties, 7 C. oleifera Abel., 2 C. osmantha, 1 C. gigantocarpa Hu et Huang, 1 C. chekiangoleosa, 1 C. gauchowensis, 1 C. fangchengensis S.Y. Liang et Y.C. Zheng, and 1 C. sinensis (L.) O. Ktze. Sample numbers are shown in Table 1.
Squalene, vitamin E, stigmasterol, β-amyrin, 3,4-dihydroxyphenylacetic acid, epicatechin, tea saponin, rutin, quercetin, camellianin A standards, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), and Trolox were purchased from Sigma–Aldrich Chemical Co. Ltd. (Shanghai, China). Other reagents and solvents were obtained from Shanghai Macklin Biochemical Co. Ltd. (Shanghai, China).

Yu J., Yan H., Wu Y., Wang Y, & Xia P. (2022). Quality Evaluation of the Oil of Camellia spp.. Foods, 11(15), 2221.

+ Open protocol

+ Expand

Integrated Gut Microbiome Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Formic acid, bile salts, soluble starch, (+)-arabinogalactan, tryptone, yeast extract, xylan from birchwood, L-cysteine hydrochloride monohydrate, guar gum, inulin, Tween 80, buffered peptone water, Dulbecco′s phosphate buffer saline (PBS), casein sodium salt from bovine milk, pectin from citrus fruits, mucin from porcine stomach-type III, CaCl₂, KCl, NaCl, NaHCO₃, anhydrous K₂HPO₄, KH₂PO₄, MgSO₄ monohydrate, FeSO₄ heptahydrate, resazurin redox indicator, quercetin, kaempferol, quercetin-3-O-rutinoside (aka rutin), phenylacetic acid, 4′-hydroxyphenylacetic acid, 3′-hydroxyphenylacetic acid, 3′,4′-dihydroxyphenylacetic acid, 3-phenylpropanoic acid, 3-(4′-hydroxyphenyl)propanoic acid, 3-(3′-hydroxyphenyl)propanoic acid, 3-(3′,4′-dihydroxyphenyl)propanoic acid (aka dihydrocaffeic acid), 4-hydroxybenzoic acid, 3-hydroxybenzoic acid, 3,4-dihydroxybenzoic acid (aka protocatechuic acid), and benzene-1,3,5-triol (aka phloroglucinol) were obtained from Sigma-Aldrich (St Louis, MO, USA). Isorhamnetin was from PhytoLab GmbH (Vestenbergsgreuth, Germany). All solvents and water were UHPLC-grade and were purchased from VWR International (Milan, Italy).

Di Pede G., Bresciani L., Calani L., Petrangolini G., Riva A., Allegrini P., Del Rio D, & Mena P. (2020). The Human Microbial Metabolism of Quercetin in Different Formulations: An In Vitro Evaluation. Foods, 9(8), 1121.

+ Open protocol

+ Expand

Chromatography of Polyphenol Standards

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chromatography
standards of (−)-epicatechin,
procyanidin B2, 3-hydroxyphenylacetic acid (HPA), 3,4-dihydroxyphenylacetic
acid (34PA), and 3-(3,4-dihydroxyphenyl)propionic acid (34PP) were
acquired from Sigma-Aldrich (St. Louis, MO). Standards of 3-(3-hydroxyphenyl)propionic
acid (HPP), 3,4-dihydroxybenzoic acid (34BA), and 3-hydroxybenzoic
acid (HBA) were acquired from Alfa Aesar (Tewksbury, MA). 5-(3′,4′-Dihydroxyphenyl)-g-valerolactone (34PV) was acquired from Toronto Chemicals
(North York, ON, Canada). Procyanidin A2 was purchased from Extrasynthese
(Lyon, France). LC-MS-grade mobile phases were also from Sigma-Aldrich,
and formic acid was from Fisher Scientific (Hampton, NH).

Diaz M.S., Mertens-Talcott S.U, & Talcott S.T. (2024). Intestinal Microbiome Metabolism of Cranberry (Vaccinium macrocarpon) Proanthocyanidin Dimers, but Not Trimers, Is Altered by Dysbiosis in Ulcerative Colitis Ex Vivo. Journal of Agricultural and Food Chemistry, 72(8), 4184-4194.

+ Open protocol

+ Expand

Analytical Methods for Phenolic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Reagents of Mass Spectrometry grade, including acetonitrile, methanol, water, and formic acid, were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Also, Folin–Ciocalteu’s phenol reagent was obtained from Sigma Chemical Co. Analyzed phenols included protocatechuic acid, m-hydroxy benzoic acid, chlorogenic acid, salicylic acid, caffeic acid, syringic acid, sinapic acid, ferulic acid, p-coumaric acid, m-coumaric acid, o-coumaric acid, gallic acid, o-hydroxy benzoic acid, 3,4-dihydroxyphenylacetic acid, trans-cinnamic acid, vanillic acid, benzoic acid, ellagic acid, vitexin, rutin, (+)-catechin, apigenin, kaempferol, orientin, naringenin, myricetin, isorhamnetin-3-O-rutinoside, isorhamnetin-3-O-glucoside, kaempferol-3-O-rutinoside, quercetin, and its derivativeswere purchased from Sigma Chemical Co. (St. Louis, MO, USA). Folin–Ciocalteu’s phenol reagent, 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2-di(4-tert-octylphenyl)-1-picrylhydrazyl (DPPH), and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) were obtained from Sigma Chemical Co. (St. Louis, MO, USA).

Sawicki T., Jabłońska M., Danielewicz A, & Przybyłowicz K.E. (2024). Phenolic Compounds Profile and Antioxidant Capacity of Plant-Based Protein Supplements. Molecules, 29(9), 2101.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!