Agilent 6890 gc ms

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 6890 GC-MS is a gas chromatograph-mass spectrometer system designed for the separation, identification, and quantification of chemical compounds. It combines a gas chromatograph for sample separation with a mass spectrometer for compound detection and analysis.

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Lab products found in correlation

Trypsin edta, by Thermo Fisher Scientific (3 mentions) Rid 10a, by Shimadzu (2 mentions) Lc 20ad, by Shimadzu (1 mentions) Pack ods a column, by YMC (1 mentions) Spd 20a, by Shimadzu (1 mentions) Hp 5ms column, by Agilent Technologies (1 mentions) Hp 5ms, by Hewlett-Packard (1 mentions) Bead beating, by Biospec (1 mentions)

Close spelling variants of this product

6890 Agilent GC with 5975 MS detector Agilent 6890-5975 GC-MS system Agilent 6890/5975 GC-MS Agilent 6890 GC/5973 MS Agilent 6890 GC-5973 MS system 6890 GC-MS Agilent 6890 GC GC 6890 Agilent 6890 GC-MS

6 protocols using agilent 6890 gc ms

Quantification of Biomass Degradation Compounds

Cited 3 times

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Glucose, xylose, and acetic acid were analyzed using HPLC (LC-20AD, refractive index detector RID-10A, Shimadzu, Kyoto, Japan) with Bio-Rad Aminex HPX-87H column at 0.6 mL/min of 5-mM sulfuric acid solution and the column temperature of 65 °C [14 (link)].
Furan and phenolic compounds were analyzed using reverse-phase HPLC (LC-20AT, SPD-20A UV detector, Shimadzu, Kyoto, Japan) equipped with YMC-Pack ODS-A column (YMC, Tokyo, Japan) [31 (link)]. All samples were filtered through the 0.22-μm membrane before HPLC analysis.
It identified the degradation intermediates of aldehyde inhibitors by A. resinae ZN1 on Agilent 6890 GC–MS (Agilent Technologies, Santa Clara, CA) with HP-5 MS column (30 m × 0.25 mm × 0.25 μm) from 80 °C (held for 4 min) to 280 °C at the rate of 8 °C/min. One microliter sample was detected under splitless condition [68 (link), 69 (link)].

Yi X., Gao Q., Zhang L., Wang X., He Y., Hu F., Zhang J., Zou G., Yang S., Zhou Z, & Bao J. (2019). Heterozygous diploid structure of Amorphotheca resinae ZN1 contributes efficient biodetoxification on solid pretreated corn stover. Biotechnology for Biofuels, 12, 126.

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Metformin Alters Metabolic Landscape

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For steady-state analysis of metabolites, HPMCs from 2 separate patients were plated at 1,000,000 cells per 15cm dish in duplicate and allowed to grow for 72h prior to metformin exposure (1mM, 48h). Cells were lifted using Trypsin EDTA (1X, 0.25%; ThermoFisher Scientific) and then pelleted by centrifugation at 0.5rcf. for 3min. Cell pellets were flash frozen in liquid nitrogen and then prepared for downstream mass spectrometric analysis, as described previously (Lai et al., 2018 (link)). The Agilent 6530 Accurate-Mass Q-TOF LC/MS quadrupole analyzer and Agilent 6890 GC/MS (Agilent, Santa Clara, CA) were used to perform untargeted metabolomics. 617 metabolites were identified in total, and of those 189 known metabolites were used in subsequent analyses. Comparisons of relative abundance were performed using Perseus (Max Planck) as described above, and significantly altered metabolites were analyzed in conjunction with proteomics data using MetaboAnalyst Joint Pathway Analysis (metaboanalyst.ca)(Chong et al., 2018 (link)) to indicate pathways altered by metformin.

Hart P.C., Kenny H.A., Grassl N., Watters K.M., Litchfield L.M., Coscia F., Blaženović I., Ploetzky L., Fiehn O., Mann M., Lengyel E, & Romero I.L. (2019). Mesothelial Cell HIF1α Expression Is Metabolically Downregulated by Metformin to Prevent Oncogenic Tumor-Stromal Crosstalk. Cell reports, 29(12), 4086-4098.e6.

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HPLC and GC-MS Analysis of Biodegradation Intermediates

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Glucose and ethanol were analyzed using HPLC equipped with a refractive index detector RID-10A (Shimadzu, Kyoto, Japan) with an Aminex HPX-87H column (Bio-rad, Hercules, CA, USA) at 65 °C using a mobile phase of 5 mM H₂SO₄ at a rate of 0.6 mL/min. 4-Hydroxybenzaldehyde, syringaldehyde, and vanillin were analyzed using reverse-phase HPLC (SPD-20A, Shimadzu, Kyoto, Japan) equipped with a YMC-Pack ODS-A column (Tokyo, Japan) at 35 °C. 4-Hydroxybenzaldehyde and syringaldehyde were detected at 270 nm using a mobile phase of 30 % acetonitrile solution at a rate of 1.0 mL/min, and vanillin was detected at 320 nm using 50 % acetonitrile solution at a rate of 0.8 mL/min.
The biodegradation intermediates of phenolic aldehydes by Z. mobilis ZM4 was identified by GC–MS. Samples were taken at 4 h intervals after inoculation and concentrated by rotary evaporator with vacuum system, then dissolved in ethyl acetate and acetonitrile solution (2:1, v/v) and silylated with NO-bis-trimethylsilyl trifluoro-acetamide according to [31 (link), 32 (link)]. The treated samples were analyzed using Agilent 6890 GC–MS fitted with an HP-5 MS column (30 m × 0.25 mm × 0.25 μm) (Agilent Technologies, Santa Clara, CA, USA) from 80 °C (held for 4 min) to 280 °C at 8 °C/min. 1 μL sample was injected and detected under splitless condition.

Yi X., Gu H., Gao Q., Liu Z.L, & Bao J. (2015). Transcriptome analysis of Zymomonas mobilis ZM4 reveals mechanisms of tolerance and detoxification of phenolic aldehyde inhibitors from lignocellulose pretreatment. Biotechnology for Biofuels, 8, 153.

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GC-MS Analysis of Chemical Compositions

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The chemical compositions of HO and CO were investigated by Agilent 6890 GC-MS (AgilentTechnologies, CA, USA), using 30.0 m × 250 mm i.d., 0.25-mm film thickness fused-silica HP-5 ms (HewlettPackard, CA, USA), and hydrogen gas was used as the carrier (mobile phase). The flow rate of the carrier gas was set at 1 mL/min, and the injection temperature was set at 260 °C. The analyses were performed by using a temperature gradient. In particular, column initial temperature was set at 100 °C, which was held for 3 min. Then, the column temperature was raised at 3 °C/min to reach the final temperature of 280 °C, which was maintained for 3 min. The resulting chromatogram was used to identify the chemical compositions of each essential oil by using the differential of the retention time compared with Wiley, NIST, and NBS libraries. Kovats index (KI) was calculated by using the following equation:
where KI is the Kovat index; tn, tn+1, and tx are the retention time (in minute) of the two n-alkanes containing n, n + 1 carbons, and the compounds of interest, respectively [19 (link)].

Laothaweerungsawat N., Sirithunyalug J, & Chaiyana W. (2020). Chemical Compositions and Anti-Skin-Ageing Activities of Origanum vulgare L. Essential Oil from Tropical and Mediterranean Region. Molecules, 25(5), 1101.

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Quantification of Chlorophyll and Alkanes

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2 mL of culture was pelleted by centrifugation and combined with 1 mL of ethyl acetate and 0.5 mL of 0.1 mm glass beads. Cells were lysed by bead beating (Biospec Products, Bartlesville, OK) for 3 cycles of 1 minute, with 5 minutes rest between cycles. Glass beads and debris were pelleted by centrifugation for 10 minutes at 16,000 × g, and then the upper ethyl acetate layer was removed for analysis. Chlorophyll a was determined on a DW-2000 spectrophotometer according to the formula [Chl a] (μg/mL) = (16.29 × A₆₆₅) − (8.24 × A₆₅₂)53 (link). Alkanes were measured on an Agilent 6890 GC-MS fitted with a 12 meter DB5-MS column as previously described2 (link) and quantified by comparison with an n-heptadecane standard (Sigma-Aldrich, St. Louis, MO).

Berla B.M., Saha R., Maranas C.D, & Pakrasi H.B. (2015). Cyanobacterial Alkanes Modulate Photosynthetic Cyclic Electron Flow to Assist Growth under Cold Stress. Scientific Reports, 5, 14894.

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Hydrocarbon Extraction and Quantification

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Hydrocarbons were extracted from 60 mL fermentation broth using 30 mL the mixture of methanol and chloroform (the ratio of methanol/chloroform was 2:1) for 24 h and then centrifuged at 10,000 rpm for 10 min. The solvent layer on the bottom of the mixture was rotary-evaporated to remove the solvent and the hydrocarbons obtained were re-dissolved by adding 1 mL of chloroform to obtain the samples for hydrocarbons measurement.
Samples were analyzed by Agilent 6890 GC–MS (Agilent Technologies, Santa Clara, CA, USA) with HP-5-MS column. The initial temperature was 50 °C and maintained for 2 min, then ramped up to 80 °C at a rate of 15 °C/min and held at 80 °C for 3 min, after that the temperature was ramped up to 280 °C at a rate of 15 °C/min and held at 280 °C for 8 min. The flow rate of the carrier gas helium was 1 mL/min. NIST MS SEARCH 2.0 library was used for qualitative analysis, and the matching degree of samples and standard products reached more than 95%. The internal calibration was 100 mg/L dodecane (C₁₂H₂₆) and the concentration of the hydrocarbons was calculated according to the ratio of the chromatographic peak area.

Xu Y.Y., Hua K.J., Huang Z., Zhou P.P., Wen J.B., Jin C, & Bao J. (2022). Cellulosic hydrocarbons production by engineering dual synthesis pathways in Corynebacterium glutamicum. Biotechnology for Biofuels and Bioproducts, 15, 29.

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