We used immune dot blot to determine the presence of anti-NXP2 antibody in human serum (D-TEK, Belgium). Creatine kinase (CK) were tested by automated biochemical analyzer (Siemens, Germany). Ferritin was measured by quantitative chemiluminescence assay (Abbott Laboratories, US). Anti-nuclear antibodies (ANA) and anti-Ro-52 antibodies were measured by immunofluorescence assay (Euroimmun, US) and immunoblotting (Euroimmun, US) respectively. We assessed CD4/CD8 ratio by flow cytometry and immunofluorescence (Becton, Dickinson and Company, US).
Immunoblotting
Manufactured by EUROIMMUN
Sourced in United States
Immunoblotting is a laboratory technique used to detect and quantify specific proteins in a complex mixture. It involves the separation of proteins by size using gel electrophoresis, followed by their transfer to a membrane and detection using antibodies specific to the protein of interest.
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Lab products found in correlation
4 protocols using immunoblotting
1
Autoimmune Biomarkers in Serum
2
Comprehensive Biomarker Analysis in IIMs
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The following additional data for patients with IIMs were collected: CK, LDH, ALT, AST, erythrocyte sedimentation rate (ESR), C‐reactive protein (CRP), and neutrophil and lymphocyte counts. These were all analyzed using routine laboratory techniques within 24 h of enrolment. Antinuclear antibody (ANA) was detected by indirect immunofluorescence using Hep‐2 cells (Euroimmun). Myositis‐specific and myositis‐associated autoantibodies were detected using immunoblotting (Euroimmun). The demographic data for the disease groups are shown in Table 1 .
3
Serum Biomarkers for Allergy and Autoimmunity
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Serum levels of tIgE (Euroimmun, Lübeck, Germany) were measured using ELISA kits according to the manufacturer’s protocols (normal range: 0–100 IU/ml). Serum sIgEs were detected using immunoblotting (Euroimmun) as previously described (10 (link)). The detected sIgEs included 8 inhalant sIgEs and 10 food sIgEs. A test result of < 0.70 kU/L was considered negative. Serum levels of anti-TPO (normal range: 0.00–5.61 IU/ml) and anti-TG (normal range: 0.00–4.11 IU/ml) IgG AAbs were measured according to the manufacturer’s protocols (Abbott Park, Middletown, USA).
4
Serum Allergen-Specific IgE Detection
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Serum allergen-specific IgEs were detected using immunoblotting (Euroimmun). The detected allergens included willow/poplar/elm, ragweed, mugwort, pteronyssinus/farinae, house dust, cats, dogs, penicillium/branch spore mould/fumigatus/alternaria, humulus, egg white, cow's milk, peanut, soybean, beef, mutton, codfish/ lobster/scallop, salmon/weever/carp, shrimp, crab. A test result of 0.7 kU/l or higher (level 2 to 6) was considered positive.
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