Sc 51313

Manufactured by Santa Cruz Biotechnology

SC-51313 is a protein assay kit that can be used to quantify total protein concentrations in a sample. The kit includes reagents and a microplate protocol that allows for colorimetric detection and measurement of protein levels.

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Lab products found in correlation

G actin f actin in vivo assay biochem kit, by Cytoskeleton (1 mentions) A7811, by Merck Group (1 mentions) Sc 208, by Santa Cruz Biotechnology (1 mentions) Sc 23918, by Santa Cruz Biotechnology (1 mentions) Anti dhpr, by Thermo Fisher Scientific (1 mentions) Ma3 920, by Thermo Fisher Scientific (1 mentions)

2 protocols using sc 51313

Analyzing Murine Cardiomyocyte Protein Expression

Cited 1 time

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Western blotting and immunostaining of left ventricular murine cardiomyocytes was performed as described previously [24 (link), 26 (link)] using antibodies against PKCα (sc-208, Santa Cruz), JP2 (SC-51313, Santa Cruz), GAPDH (2118, Cell Signaling) and α-actinin (A7811, Sigma). Immunofluorescence imaging for α-actinin and JP2 was performed using a confocal microscope (Carl Zeiss LSM 510 MicroImaging) as previously described.[27 (link)] Cellular fractions of G-actin and F-actin were determined by the G-Actin/F-actin In Vivo Assay Biochem Kit (Cytoskeleton, Inc) per the manufacturer’s instructions.

Guo A., Chen R., Wang Y., Huang C.K., Chen B., Kutschke W., Hong J, & Song L.S. (2018). Transient activation of PKC results in long-lasting detrimental effects on systolic [Ca2+]i in cardiomyocytes by altering actin cytoskeletal dynamics and T-tubule integrity. Journal of molecular and cellular cardiology, 115, 104-114.

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Immunoblotting Analysis of Quadriceps Muscle Proteins

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Proteins in lysates of 2-3month old wildtype and Ehd1-heterozygous quadriceps muscles, (n ≥ 2), were transferred to membranes and were immunoblotted with anti-BIN1 (1:1000, sc-23918, Santa Cruz, Dallas, TX), rabbit polyclonal anti-EHD1 (1:5000, [10 (link)]), anti-DHPR (1:1000, ma3-920, Thermo Scientific, Rockford, IL), anti-Junctophilin 2 (1:1000, sc-51313, Santa Cruz, Dallas, TX), anti-Junctophilin 1 (1:1000, 40–5100, Thermo Scientific, Rockford, IL) antibodies. Secondary antibodies, goat anti-rabbit, and goat anti-mouse conjugated to horseradish peroxidase (Jackson ImmunoResearch) were used at a dilution of 1:2500. Blocking and antibody incubations were done in Starting Block T20 (Invitrogen, Grand Island, NY) for all antibodies and rinsed with TBS-T. Memcode and gelcode stains were used to mark proteins for loading controls (Life Technologies, Grand Island, NY). Chemiluminescence substrate, Kodak Biomax MS film, and a UVP BioSpectrum Imaging System (Upland, CA) were used for detection.

Demonbreun A.R., Swanson K.E., Rossi A.E., Deveaux H.K., Earley J.U., Allen M.V., Arya P., Bhattacharyya S., Band H., Pytel P, & McNally E.M. (2015). Eps 15 Homology Domain (EHD)-1 Remodels Transverse Tubules in Skeletal Muscle. PLoS ONE, 10(9), e0136679.

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