In carta image analysis software
IN Carta image analysis software is a digital pathology tool that enables users to view, analyze, and manage digital slide images. The software provides a platform for image processing and quantitative analysis of microscopic samples.
10 protocols using in carta image analysis software
Quantifying Cellular Acetyl-Tubulin Levels
Quantifying Oxidative Stress by Nitrotyrosine
Quantitative Analysis of Nuclear Morphology
Evaluating Cell Membrane Integrity
Quantifying DNA Damage via γ-H2AX
Quantifying Cellular Hsp70 Levels
Mitochondrial Membrane Potential Assay
Genotoxicity Evaluation of Test Compounds
Mitochondrial Membrane Potential Analysis
Assessing Cell Membrane Integrity by PI Staining
Subsequently, cells were washed with PBS and stained with 3 μM calcein-AM (Thermo Fisher Scienti c) and propidium iodide (5 μM) followed by incubation for 30 min. 100 µl of cell suspension was transferred to black plates and imaged with uorescent microscope (IN Cell Analyzer 2000) using excitation of 475 nm /emission of 511 nm for calcein-AM and excitation of 542 nm /emission of 620 nm for PI. Generated images were automatically analysed using IN Carta Image Analysis Software (GE Healthcare).
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