The 1H (400 MHz) and 13C (100 MHz)-NMR spectra were recorded by on a JEOL (Freising, Germany) spectrometer using CD3OD and DMSO-d6 as solvents and tetramethylsilane (TMS) as an internal standard. For column chromatography: normal-phase silica gel 60 (230–400 mesh, Merck KGaA, Darmstadt, Germany) and Sephadex LH-20 (Sigma Aldrich® a subsidiary of Merck KGaA, Darmstadt, Germany) were used. Merck precoated silica gel F254 plates were used for analytical TLC purpose. SIL G-25 Unmodified Standard Silica Layer Glass Plates with thickness of 2 mm (Macherey-Nagel®, Düren, Germany) were used for preparative TLC. Visualization of TLC was carried out using UV light and anisaldehyde—sulphuric acid spray reagent. Cisplatin (Cisplatine®) was obtained from Mylan Company (Saint Priest, France). Phosphate buffered saline solution (PBS) was purchased from Lonza Bio-products (Verviers, Belgium).
Precoated silica gel f254 plates
Manufactured by Merck Group
Precoated silica gel F254 plates are thin-layer chromatography (TLC) plates with a pre-applied layer of silica gel on a solid support, such as glass or aluminum. The silica gel layer acts as the stationary phase for separation of compounds. The F254 indicator is added to the silica gel, which allows for visualization of separated compounds under ultraviolet (UV) light at 254 nm.
Automatically generated - may contain errors
Lab products found in correlation
Sephadex lh 20, by Merck Group (1 mentions)
Normal phase silica gel 60, by Merck Group (1 mentions)
Sil g 25 unmodified standard silica layer glass plates, by Macherey-Nagel (1 mentions)
Phosphate buffered saline solution pbs, by Lonza (1 mentions)
Ft ir 4100, by Jasco (1 mentions)
Flash ea 1112, by Thermo Fisher Scientific (1 mentions)
Avance 3 400, by Bruker (1 mentions)
Avance 500 mhz spectrometer, by Bruker (1 mentions)
2 n 7 nitrobenz 2 oxa 1 3 diazol 4 yl amino 2 deoxyglucose 2 nbdg, by Thermo Fisher Scientific (1 mentions)
Silica gel, by Merck Group (1 mentions)
Exactive mass spectrometer, by Thermo Fisher Scientific (1 mentions)
4 protocols using precoated silica gel f254 plates
1
Characterization of Chemical Compounds
2
Synthesis and Characterization of Novel Compounds
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Completion of reaction was monitored by thin-layer chromatography on Merck precoated silica gel F-254 plates. Melting points of the synthesized compounds were determined on Veego VMP-D digital melting point apparatus by open capillary method and are uncorrected. The IR spectra of the synthesized compounds were recorded on Jasco FT-IR 4100 in potassium bromide. The 1H-NMR, 13C-NMR, and 13C-DEPT-135 spectra were recorded in deuterated chloroform (CDCl3) and dimethylsulfoxide (DMSO) using NMR Varian Mercury plus 300 MHz using tetramethylsilane (TMS) as internal standard. The mass spectra were recorded on 410 Prostar Binary LC with a 500 MS IT PDA detector. Elemental analysis was performed on FLASH EA 1112, Thermo-Finnigan, and indicated with the element symbol. The elemental analyses were within ± 0.4% of the theoretical values.
3
Characterization of Organic Compounds
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All reagents were obtained from local suppliers and were used without further purification. All reactions were monitored by thin-layer chromatography with pre-coated silica gel F254 plates purchased from Merck, Inc. All melting points were determined on Yanaco Melting point apparatus and were uncorrected. Mass spectra (MS) were taken in ESI mode on Agilent Technologies LC/MSD TOF instruments. 1H NMR spectra were recorded on Varian Mercury 300 MHz or 400 MHz spectrometer. 13C NMR spectra were recorded on Bruker AVANCEIII 400 or 600 MHz spectrometer.
4
NMR, HRESIMS, and Column Chromatography Protocol
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NMR spectra were recorded on a Bruker Avance 500 MHz spectrometer, using acetone-d 6 as solvent and the chemical shifts are expressed in δ (ppm) relative to the tetramethylsilane peak.
HRESIMS experiments were conducted on a Thermo Scientific Exactive mass spectrometer.
Column chromatography was performed using silica gel (100-200 mesh; Merck, Darmstadt, Germany). Merck pre-coated silica gel F 254 plates were used for monitoring thin-layer chromatography (TLC). Spots were visualized under UV light (254 and 365 nm) and by spraying with anisaldehyde-sulfuric acid solution followed by heating.
-Amylase, -glucosidase, acarbose, bovine serum albumin (BSA), -D-glucose, ascorbic acid, metformin, 4-nitrophenyl-α-D-glucopyranoside (p-NPG), Dulbecco's modified Eagle's media (DMEM), fetal bovine serum (FBS), antibiotic-antimycotic solution (Pencillinstreptomycin-amphotericin B mix) and 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were procured from M/s Sigma-Aldrich Chemicals (St. Louis, MO, USA). 2-(N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG) was procured from molecular probe (Invitrogen Life Technologies, Carlsbad, CA, USA). Phospho-specific or panspecific antibodies against AMPK, and GLUT 4 were purchased from Santa Cruz Biotechnology, United States. All other chemicals and reagents used were of highest grade available.
HRESIMS experiments were conducted on a Thermo Scientific Exactive mass spectrometer.
Column chromatography was performed using silica gel (100-200 mesh; Merck, Darmstadt, Germany). Merck pre-coated silica gel F 254 plates were used for monitoring thin-layer chromatography (TLC). Spots were visualized under UV light (254 and 365 nm) and by spraying with anisaldehyde-sulfuric acid solution followed by heating.
-Amylase, -glucosidase, acarbose, bovine serum albumin (BSA), -D-glucose, ascorbic acid, metformin, 4-nitrophenyl-α-D-glucopyranoside (p-NPG), Dulbecco's modified Eagle's media (DMEM), fetal bovine serum (FBS), antibiotic-antimycotic solution (Pencillinstreptomycin-amphotericin B mix) and 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were procured from M/s Sigma-Aldrich Chemicals (St. Louis, MO, USA). 2-(N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG) was procured from molecular probe (Invitrogen Life Technologies, Carlsbad, CA, USA). Phospho-specific or panspecific antibodies against AMPK, and GLUT 4 were purchased from Santa Cruz Biotechnology, United States. All other chemicals and reagents used were of highest grade available.
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