Disulfiram

We used the Prestwick drug library of FDA-approved compounds, consisting of 1200 compounds from Prestwick (Illkirch, Alsace, France), for drug screening. The following compounds of Disulfiram, Auranofin, Thiostrepton, Thimerosal, Halofantrine, Vorinostat, CDDO-me, RTA-408, oleic acid, and palmitic acid were purchased from Cayman Chemicals (Ann Arbor, MI, USA). Hydrogen Peroxide was obtained from MilliporeSigma (Burlington, MA, USA).

Cisplatin [cis-diammineplatinum(II) dichloride], was obtained from Sigma-Aldrich (St. Louis, MO, USA) and dissolved in DMSO. Disulfiram was obtained from Cayman Chemical and dissolved in DMSO. Aliquots were stored at −20 °C for up to a maximum of three months and thawed immediately before use. WST-1 [2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium] was purchased from ScienCell (Research Lab., Carlsbad, CA, USA) freshly made, #8038; RIPA lysis buffer was purchased from VWR Chemicals, #N653-100 mL; protease inhibitor was purchased from Thermo Fisher Scientific (Waltham, MA, USA), #PIER87785; BCA purchased from Thermo Fisher Scientific, #PIER23225. Primary antibodies: E cadherin #3195P cell signaling, N cadherin #13116P cell signaling, ABCC1 #VMA00330 BioRad (Hercules, CA, USA), ABCC4 #PA5-18315 Thermo Fisher Scientific, ABCG2 #BRB155559 Biorbyt (Cambridge, UK), GAPDH #sc-32233 Santa Cruz Biotechnology (Dallas, TX, USA), α-Tubulin #NB100-690H Novus Biologicals (Littleton, CO, USA), ALDH3A1 #PA5-80332 Thermo Fisher Scientific, ALDH7A1 #MA5-29028 Thermo Fisher Scientific. Secondary HRP-conjugated antibodies were purchased from Santa Cruz Biotechnology.