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Genjet plus dna in vivo tranfection reagent

Manufactured by SignaGen
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Sourced in United States

GenJet™ Plus DNA In Vivo Tranfection Reagent is a laboratory product designed for the transfection of DNA into cells. It facilitates the delivery of DNA into cells for various research applications.

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Lab products found in correlation

In vivo jetpei, by Polyplus Transfection (1 mentions) 5 triphosphate double stranded rna 5 ppp dsrna, by InvivoGen (1 mentions)

Close spelling variants of this product

GenJet (38 citations) GenJet reagent (9 citations) GenJet™ Plus reagent (7 citations) GenJet Plus (3 citations)

2 protocols using genjet plus dna in vivo tranfection reagent

1

In Vivo H5N1 Influenza Virus Propagation

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GenJet™ Plus DNA In Vivo Tranfection Reagent was purchased from SignaGen (Rockville, MD, USA) and in vivo-jetPEI® was purchased from Polyplus Transfection (New York, NY). H5N1 Monovalent Influenza Split Vaccine (A/Indonesia/05/2005, A/IN/05) was provided by GlaxoSmithKline Vaccines (Ste-Foy, Quebec, Canada). RG H5N1 A/IN/05 virus was made by reverse genetics with HA and NA from A/IN/05 virus and the remaining six gene segments from A/Puerto Rico/8/1934 (PR8) virus. Viruses were propagated in 10-day-old embryonated chicken eggs for 48 h. Pooled allantoic fluids were clarified by centrifugation, aliquoted, and stored at –80 °C until use.
Cao W., Mishina M., Amoah S., Mboko W.P., Bohannon C., McCoy J., Mittal S.K., Gangappa S, & Sambhara S. (2018). Nasal delivery of H5N1 avian influenza vaccine formulated with GenJet™ or in vivo-jetPEI® induces enhanced serological, cellular and protective immune responses. Drug Delivery, 25(1), 773-779.
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2

Synthetic RIG-I Agonist and Recombinant HA Protein

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5' triphosphate double stranded RNA (5' ppp-dsRNA), a synthetic ligand for retinoic acid-inducible protein (RIG-I), was purchased from Invivogen (San Diego, CA). The biological activity and absence of bacterial contamination of 5' ppp-dsRNA have been confirmed by the manufacturer. GenJet™ Plus DNA In Vivo Tranfection Reagent (SignaGen, CA) was used to deliver 5'ppp-dsRNA. Recombinant HA protein from A/Shanghai/2/2013 (rH7HA) influenza virus was expressed and purified using the baculovirus expression vector system, as described previously [11 (link)].
Cao W., Liepkalns J.S., Kamal R.P., Reber A.J., Kim J.H., Hofstetter A.R., Amoah S., Stevens J., Ranjan P., Gangappa S., York I.A, & Sambhara S. (2016). RIG-I ligand enhances the immunogenicity of recombinant H7 HA protein. Cellular immunology, 304-305, 55-58.
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