Log In Sign up
The largest database of trusted experimental protocols

Hrp linked goat anti actin

Manufactured by Santa Cruz Biotechnology
Visit Supplier

HRP‐linked goat anti‐Actin is a laboratory reagent used to detect the presence of the actin protein in biological samples. It consists of an antibody specific to the actin protein that is conjugated with the enzyme horseradish peroxidase (HRP). This reagent can be used in various immunoassay techniques to measure the levels of actin in cells or tissues.

Automatically generated - may contain errors

Lab products found in correlation

Femto chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) Mini protean tgx precast gradient gel, by Bio-Rad (2 mentions) Protease inhibitor cocktail 5, by Merck Group (2 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) Rabbit anti phospho mtor, by Cell Signaling Technology (1 mentions) Horseradish peroxidase hrp linked anti rabbit igg pab, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Goat anti-actin-HRP Anti-actin-HRP Goat anti-actin Actin-HRP Anti-actin

2 protocols using hrp linked goat anti actin

1

Phosphorylation Analysis of mTOR and MERTK

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cultured cells were lysed in buffer containing NaF, Na4P2O7, Na3VO4, and protease inhibitor cocktail V (EMD Millipore, Temecula, CA) to preserve protein phosphorylation. Proteins were resolved on Mini‐PROTEAN TGX precast gradient gels (BioRad, Berkeley, CA), transferred to polyvinylidene fluoride membranes, and incubated with rabbit anti‐phospho‐mTOR (monoclonal antibody [mAb]; Cell Signaling Technology, Danvers, MA), anti‐mTOR (mAb; Cell Signaling Technology), anti‐phospho‐MERTK (mer receptor tyrosine kinase; polyclonal antibody [pAb]; FabGennix, Frisco, TX), or anti‐MERTK (pAb; FabGennix). The blottings were then incubated with horseradish peroxidase (HRP)‐linked anti‐rabbit immunoglobulin G (pAb; Cell Signaling Technology) and HRP‐linked goat anti‐Actin (Santa Cruz Biotechnology, Santa Cruz, CA) and visualized with an 80/20 mix of SuperSignal West Pico Chemiluminescent Substrate and Femto Chemiluminescent Substrate (Thermo Scientific, Rochester, NY). Densitometry was performed using ImageJ analysis software (National Institutes of Health, Bethesda, MD).
Labonte A.C., Sung S.J., Jennelle L.T., Dandekar A.P, & Hahn Y.S. (2016). Expression of scavenger receptor‐AI promotes alternative activation of murine macrophages to limit hepatic inflammation and fibrosis. Hepatology (Baltimore, Md.), 65(1), 32-43.
+ Open protocol
+ Expand
2

Phosphorylation of mTOR and MERTK

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cultured cells were lysed in buffer containing NaF, Na4P2O7, Na3VO4, and protease inhibitor cocktail V (EMD Millipore, Temecula, CA) to preserve protein phosphorylation. Proteins were resolved on Mini-PROTEAN TGX precast gradient gels (BioRad, Berkeley, CA), transferred to PVDF membranes, and incubated with rabbit anti-phospho-mTOR (mAb, Cell Signaling Technology, Danvers, MA), anti-mTOR (mAb, Cell Signaling Technology), anti-phospho-MERTK (pAb, FabGennix, Frisco, TX), or anti-MERTK (pAb, FabGennix). The blots were then incubated with horseradish peroxidase (HRP)-linked anti-rabbit IgG (pAb, Cell Signaling Technology) and HRP-linked goat anti-Actin (Santa Cruz Biotechnology, Santa Cruz, CA) and visualized with an 80/20 mix of SuperSignal West Pico Chemiluminescent Substrate and Femto Chemiluminescent Substrate (Thermo Scientific, Rochester, NY). Densitometry was performed using ImageJ analysis software (National Institute of Health, Bethesda, MD).
Labonte A.C., Sung S.J., Jennelle L.T., Dandekar A.P, & Hahn Y.S. (2016). Expression of scavenger receptor‐AI promotes alternative activation of murine macrophages to limit hepatic inflammation and fibrosis. Hepatology (Baltimore, Md.), 65(1), 32-43.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!