Panc 28

The pancreatic cancer cell lines (Pan02, MIA PaCa-2, BxPC-3, PANC-1, Panc-28, SW1990, Capan-2) were obtained from ATCC (Manassas, VA, USA). CHO and HEK293 were purchased from the National Infrastructure of Cell Line Resource (Shanghai, China). HEK293, Pan02, BxPC-3, PANC-1 and Panc-28 were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) with 100 U/mL of penicillin and 100 µg/mL of streptomycin (Gibco, Carlsbad, CA, USA). MIA PaCa-2 was grown in DMEM supplemented with 10% FBS and 2.5% horse serum (Gibco, Carlsbad, CA, USA). SW1990 was maintained in L15 medium supplemented with 10% FBS. Capan-2 was cultured in RPMI-1640 (Gibco, Carlsbad, CA, USA) supplemented with 10% FBS. CHO was grown in DMEM-F12 (Gibco, Carlsbad, CA, USA) supplemented with 10% FBS. All cells were maintained at 37 °C in a humidified 5% CO₂ atmosphere. Cell lines were identified by short tandem repeat analysis and checked for mycoplasma contamination.

HepG2 and SK-HEP-1 (Hepatocellular carcinoma) cell lines were cultured in Eagle’s Minimum Essential Medium (EMEM) medium. CA-46 (Burkitt’s lymphoma), SU-DHL-1 (Anaplastic Large Cell Lymphoma), and KMH2 (Hodgkin lymphoma) cell lines were cultured in RPMI-1640 medium (GIBCO, Grand Island, NY, USA); PANC-28 (Pancreatic Cancer), Hey (Ovarian Cancer), DU145, 22RV1, LNCAP, and PC3 (Prostate cancer) cell lines were cultured in Dulbecco's Minimum Essential Medium (DMEM) supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS) (GIBCO) and 100 IU/mL penicillin-streptomycin. All the cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).
Dulbecco’s Phosphate-Buffered Saline (DPBS; Sigma, St. Louis, MO, USA) enriched with 4.5 g/L glucose and 5 mM MgCl₂ was used as washing buffer during selection and flow cytometry assays. The binding buffer, comprising washing buffer with 1 mg/mL Bovine Serum Albumin (BSA; Fisher, Waltham, MA, USA) and 0.1 mg/mL t-RNA, was used to reduce nonspecific background binding.