Anti p44 42 mapk erk1 2 t202 y204

Manufactured by Cell Signaling Technology

Anti-p44/42 MAPK (Erk1/2) T202/Y204 is an antibody product designed to detect the phosphorylation of p44/42 MAPK (Erk1/2) at threonine 202 and tyrosine 204. This antibody is used for the analysis of MAPK activation by Western blotting.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using anti p44 42 mapk erk1 2 t202 y204

Antibody Characterization for Cell Signaling Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies used in this study are as follows. Anti-human Claspin was generated against the human recombinant Claspin with aa896–1,014 produced in E. coli. Anti-Chk1 phospho-S345 (#2348), anti-Chk1 phospho-S317 (#2344), anti-p44/42 MAPK (Erk1/2) (#4695), anti- SAPK/JNK (#9252), p38 MAPK (#8690), anti-p38 MAPK T180/Y182 (#4511), anti-p44/42 MAPK (Erk1/2) T202/Y204 (#4370), anti-SAPK/JNK T183/Y185 (#4668), Caspase-9 (#9508), Cleaved Caspase-3(#9661), and Mcl-1 (#5453) were obtained from Cell Signaling. Anti-α Tubulin (sc23948), anti-MCM2 (sc-9839), and anti-Chk1 (sc-8408) were obtained from Santa Cruz. Anti-phospho-H2A.X S139 (06-536) was purchased from Merck. Anti-BrdU (Ab6326) was purchased from Abcam. Anti-ATR phospho-T1989 (GTX128145) was purchased from GeneTex. Anti-BrdU (555627) was purchased from BD Pharmingen. Anti-H2A.X phospho-S139 (613402), anti-Rat IgG Alexa Fluor 555 (405420), and FITC-Anti-BrdU (364104) were purchased from Biolegend. RPA32 phospho-S4/S8 (A300-245A) and anti-MCM2 S53(A300-756A) were purchased from Bethyl. Anti-Mouse IgG Alexa Fluor 488 (A-11017) was purchased from Invitrogen. Goat Anti-Rabbit IgG HRP (111-035-003) and Goat Anti-Mouse IgG (115-035-003) were purchased from Jackson ImmunoResearch Laboratory.

Hsiao H.W., Yang C.C, & Masai H. (2023). Claspin-Dependent and -Independent Chk1 Activation by a Panel of Biological Stresses. Biomolecules, 13(1), 125.

+ Open protocol

+ Expand

Antibody Use for Cellular Protein Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies used in this study are as follows. Anti-human Claspin, anti-mouse Claspin, anti-MCM4, and anti-MCM4 S6T7 were previously described.¹⁹ (link) Anti-LaminB (sc-6216), anti-Chk1 (sc-8408), anti-p53 (sc-99), anti-Claspin (sc-376773) and anti-MCM2 (sc-9839) were from Santa Cruz Biotechnology; anti-Flag (M185-3L) were from MBL; anti-Chk1 S345(#2341 or #2348), anti-Chk1 S317 (#2344), anti-mTor (#2983), anti-mTOR S2448 (#5536), anti-p44/42 MAPK (Erk1/2) (#4695), anti- SAPK/JNK (#9252), p38 MAPK (#8690), anti-p38 MAPK T180/Y182 (#4511), anti-p44/42 MAPK (Erk1/2) T202/Y204 (#4370), anti-SAPK/JNK T183/Y185 (#4668), anti-PDK1 (#3062), anti-PDK1 S241 (#3438), anti-PI3 kinase p110β (#3011), anti-PI3 kinase p110γ (#5405), anti-PI3 kinase p110α (#4249), anti-PI3 kinase class III (#3358), anti- p70 S6 kinase T389 (#9234), anti-p70 S6 kinase (#9202), anti-4E-BP1 T37/T46 (#2855), anti-4E-BP1 (#9644), anti-Caspase-3 (#14220), anti-Akt S473(#4058) and anti-Akt (#4691) were from Cell signaling; anti-tubulin (T5168) was from Sigma-Aldrich; anti-MCM2 S53(A300-756A) was from Bethyl; anti-p53 S37 (PAB12641) was from Abnova; anti-histone H2A.X Ser139 (05-636) and anti-histone H3 Ser10 (05-598) were from Merck Millipore; anti-Rb (GTX50459) was from GeneTex.

Yang C.C, & Masai H. (2023). Claspin is Required for Growth Recovery from Serum Starvation through Regulating the PI3K-PDK1-mTOR Pathway in Mammalian Cells. Molecular and Cellular Biology, 43(1), 1-21.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!