Nitrate/nitrite contents in the liver tissue homogenate were measured using a colorimetric assay kit (ZellBio GmbH, Ulm-Germany) as indicated in the manufacturer's instructions.
Colorimetric assay kit
Manufactured by ZellBio
Sourced in Germany
The Colorimetric assay kit is a laboratory tool designed to quantify the presence and concentration of specific analytes in a sample. The kit utilizes a colorimetric reaction to generate a measurable color change, which can be analyzed using spectrophotometric techniques. The core function of the kit is to provide a standardized and reliable method for the quantitative analysis of target molecules in a variety of sample types.
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Lab products found in correlation
3 protocols using colorimetric assay kit
1
Quantifying Nitrate/Nitrite in Liver Tissue
2
Serum PON-1 Activity Quantification
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Fasting blood samples were collected from all patients into simple tubes prior to the operation and given enough time to make a clot. The tubes were subsequently centrifuged at 1,000 rpm for 3-5 min to form a clear serum, which was stored at -70°C. Serum PON-1 activity was measured using a colorimetric assay kit (ZellBio, Lonsee, Germany) according to the manufacturer's instructions.
3
Quantifying Kidney Tissue Damage Biomarkers
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Utilization of Pars Azmoon's kits (from Iranian company) the levels of serum creatinine (Cr), blood urea nitrogen (BUN) and proteinuria were measured quantitatively. We determined nitrite oxide stable metabolite and nitrite level in serum and supernatant via the Griess reaction of colorimetric assay kit (ZellBio, Germany). Homogenized tissue supernatant and serum level of malondialdehyde (MDA) were calculated manually (19, (link)20) . Before tissue staining we use 10% formalin for fixation and paraffin. We colored tissue sections with hematoxylin-eosin staining prior to their examination. Then two pathologists assessed the slides blindly. Tissue pathology was assessed by vehemence of distal tubular damages, transparent renal cast, wastages and debris, flattening and demolition of tubular cell, vacuolization and widening of tubular lumen and scored from 1 to 4. Overall score was determined as kidney tissue damage score (KTDS) which was reported 1 to 4, whereas natural tubes without any damage considered zero scores.
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