1260 series liquid chromatography system

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 1260 series liquid chromatography system is an analytical instrument designed for the separation, identification, and quantification of chemical compounds in complex mixtures. It features a modular design that allows for customization to meet specific analytical requirements. The system includes components such as a solvent delivery system, an autosampler, a column compartment, and a variety of detectors to monitor the separation process. The 1260 series is a versatile platform suitable for a wide range of applications in various industries, including pharmaceutical, environmental, and food analysis.

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Lab products found in correlation

C18 column, by Phenomenex (1 mentions) Agilent 6410 triple quadruple mass spectrometer, by Agilent Technologies (1 mentions) Masshunter workstation, by Agilent Technologies (1 mentions) Masshunter software, by Agilent Technologies (1 mentions) Poroshell 120 analytical column, by Agilent Technologies (1 mentions) 6420 triple quadrupole mass spectrometer, by Agilent Technologies (1 mentions)

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1260 series (163 citations) 1260 system (67 citations) 1260 liquid chromatography system (24 citations) 1260 series system (19 citations) Agilent 1260 series liquid chromatography system (4 citations) Liquid chromatography system (3 citations) 1260 series liquid chromatography (2 citations) 1260 Series Rapid Resolution Liquid Chromatography system (2 citations)

6 protocols using 1260 series liquid chromatography system

HPLC-Based Mycotoxin Analysis Protocol

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HPLC analyses were conducted using an Agilent 1260 series liquid chromatography system equipped with a fluorescence detector (Agilent, Santa Clara, CA, USA). A photochemical reactor (Huaan Magnech Bio Tech, Beijing, China) was also used for enhanced photochemical derivatization (PCD) detection. Empty columns for the fabrication of AAC columns were procured from Biocomma (Shenzhen, China). Commercial IACs for aflatoxins and mycotoxins were obtained from Romer Labs (Washington, DC, USA). The C18 column (150 mm × 4.6 mm i.d., particle size 5 µm) for HPLC separation was sourced from Phenomenex (Guangzhou, China). The benchtop rotary evaporator for removing solvent was provided by Heidolph (Shanghai, China).

Ji C., Sun X., Fang Y, & Li P. (2024). Determination of Aflatoxin B1 in Grains by Aptamer Affinity Column Enrichment and Purification Coupled with High Performance Liquid Chromatography Detection. Foods, 13(5), 640.

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Fenofibrate Quantification by HPLC

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Agilent Technologies 1260 Series liquid chromatography system with Clarity chromatography software was used. Gradient method: mobile phase A ammonium formate 10 mM, pH 3 in water; mobile phase B ammonium formate 10 mM, pH 3 in acetonitrile/water 9:1, time 0, 70%A:30%B, 3 min 0%A:100%B, 4 min 0%A:100%B, 4.5 min 70%A:30%B, total run 8 min. Column × Bridge C18 column/186003108/50 mm × 2.1 mm id. 5 μm was used. Fenofibrate (retention time 3.6 min) calibration curve (n = 6 concentrations) was run for the excipient-free DoE and each excipient concentration, with the lowest linear regression coefficient of 0.9979 for the low chitosan excipient concentration system. The method has been reported previously [13 (link),21 (link)].

Ainousah B.E., Khadra I, & Halbert G.W. (2023). Excipient Impact on Fenofibrate Equilibrium Solubility in Fasted and Fed Simulated Intestinal Fluids Assessed Using a Design of Experiment Protocol. Pharmaceutics, 15(10), 2484.

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ATRA Quantification by LC-MS/MS

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The concentrations of ATRA in plasma samples were determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis, and acitretin was used as the internal standards (IS). The LC-MS/MS system consisted of an Agilent 1260 series liquid chromatography system and an Agilent 6410 triple-quadruple mass spectrometer equipped with an Electrospray Ionization (ESI) source (Agilent Technologies, California, USA). Agilent MassHunter workstation (version B. 03. 01) was used for the control of LC-MS/MS system and data acquisition. The chromatographic analysis of ATRA and acitretin was performed on Welch ultimate XB-c18 column (2.1 × 50 mm, 3.5 μm) at 30°C. An isocratic elution was adopted with phase A (water containing 0.00625% ammonia solution) and phase B (methanol) in the ratio of 33:67. The total run time was 5 min and the injection volume was 3 μL. After chromatographic separation, the eluent was introduced to the mass spectrometer and determined in positive multiple reactions monitoring (MRM) mode with a dwell time of 500 ms. The precursor ion and product ion for ATRA and IS were m/z 299.2–255.2, and m/z 325.2–266.2, respectively. The optimized MS/MS conditions were as follows: fragmentor, 115 V; collision energy, 8 eV; capillary voltage, 3500 V; nebulizer gas pressure, 30 PSI; gas flow, 10 L/min; gas temperature, 325°C.

Yang D., Luo W., Wang J., Zheng M., Liao X.H., Zhang N., Lu W., Wang L., Chen A.Z., Wu W.G., Liu H., Wang S.B., Zhou X.Z, & Lu K.P. (2017). A novel controlled release formulation of the Pin1 inhibitor ATRA to improve liver cancer therapy by simultaneously blocking multiple cancer pathways. Journal of controlled release : official journal of the Controlled Release Society, 269, 405-422.

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Quantitative Polyphenol Analysis by LC-MS/MS

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Samples and standards were analyzed using a 1260 Series liquid chromatography system (Agilent Technologies, Santa Clara, CA, USA) coupled with a 6460A Triple Quad tandem mass spectrometer (Agilent Technologies, Santa Clara, CA, USA) with an electrospray ion source (Agilent Technologies) by the analytical conditions reported in an earlier study [86 (link)]. This coupled system was controlled using MassHunter software (version B.06.00, Agilent Technologies). Authentic polyphenol standards were: gallic acid, protocatechuic acid, p-hydroxybenzoic acid, chlorogenic acid, syringic acid, vanillic acid, caffeic acid, p-coumaric acid, ellagic acid, trans-cinnamic acid, ferulic acid, vanillin, quercetin, rutin, catechin, kaempferol, and resveratrol.

Kozarski M., Klaus A., Špirović-Trifunović B., Miletić S., Lazić V., Žižak Ž, & Vunduk J. (2024). Bioprospecting of Selected Species of Polypore Fungi from the Western Balkans. Molecules, 29(2), 314.

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HPLC Analysis of Compounds

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HPLC was performed using an Agilent Technologies 1260 Series Liquid Chromatography system controlled by Clarity Chromatography software. Mobile Phase: A: 10 mM Ammonium formate pH 3.0 in water; B: 10 mM Ammonium formate pH 3.0 in MeCN/H2O (9:1 v/v), Flow rate 1.5 mL/min, Gradient: Time 0, 70%A:30%B, 3 min 0%A:100%B, 4 min 0%A:100%B, 4.5 min 70%A:30%B total run time 10 min; Column: ACE 3 μm C18,: 50 × 3.0 mm, Column Temperature: 60 °C, Injection volume: 10 μL, Detection: 214 nm.

Zhou Z., Dunn C., Khadra I., Wilson C.G, & Halbert G.W. (2017). Statistical investigation of simulated fed intestinal media composition on the equilibrium solubility of oral drugs. European Journal of Pharmaceutical Sciences, 99, 95-104.

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Iohexol Analysis in Plasma Using LC-MS/MS

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An Agilent 1260 series liquid chromatography system paired with a 6420 triple quadrupole mass spectrometer (Agilent Technologies, Santa Clara, CA) was used to analyze plasma samples. The column temperature was maintained at 20°C. Mobile phases consisted of 0.1% formic acid in water (mobile phase A) and acetonitrile (mobile phase B) for both methods. For the analysis of iohexol, a Poroshell 120 analytical column was used (2.7 μm, 100 by 3.0 mm, part number 695975-302; Agilent Technologies). A gradient method was used to separate the analytes with solvent compositions of 5% mobile phase A-95% mobile phase B (0 to 4 min) and 97% mobile phase A-3% mobile phase B (4.1 to 7 min), at a mobile phase flow rate of 0.6 mL/min. Multiple-reaction monitoring mode was used for analyte detection, and the transitions monitored were m/z 821.8 to 803.8 and m/z 826.8 to 808.8 for iohexol and iohexol-d 5 , respectively.

Chang J., Pais G.M., Engel P.L., Klimek P., Marianski S., Valdez K, & Scheetz M.H. (2023). Impact of Vancomycin Loading Doses and Dose Escalation on Glomerular Function and Kidney Injury Biomarkers in a Translational Rat Model. Antimicrobial agents and chemotherapy, 67(2).

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