Anti opa1

Human NSCLC cell lines (Calu-1, A549, and H460) and BEAS-2B normal bronchial epithelial cells were purchased from the Cell Bank of Chinese Academy of Sciences (Shanghai, China) and grown in RPMI-1640 medium (Gibco, Grand Island, NY, USA), supplemented with 10% heat-inactivated fetal bovine serum and 100 U/mL penicillin and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) in a humidified atmosphere of 95% air and 5% CO₂ at 37°C. Recombinant human TRAIL was purchased from PeproTech (Rocky Hill, NJ, USA). Mouse monoclonal anti-Drp1, anti-phosphorylated (p)-Drp1(Ser616), anti-caspase-3, anti-PARP, anti-LC3, anti-p62, anti-COX IV, anti-PINK1, and anti-Parkin were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse monoclonal anti-Fis1, anti-Mfn1, anti-Mfn2, anti-Opa1, anti-β-actin, N-acetyl-l-cysteine (NAC), and mitochondrial division inhibitor 1 (Mdivi-1) were purchased from Sigma (St Louis, MO, USA). Mn(III) tetrakis (1-methyl-4-pyridyl) porphyrinpentachloride (MnTMPyP) was obtained from Cayman Chemical (Ann Arbor, MI, USA).