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Tlr3 pe

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TLR3-PE is a monoclonal antibody that binds to Toll-like receptor 3 (TLR3), a pattern recognition receptor involved in the detection of viral double-stranded RNA. The antibody is conjugated with the fluorescent dye phycoerythrin (PE), allowing for the detection and quantification of TLR3-expressing cells by flow cytometry.

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Lab products found in correlation

Facscalibur, by BD (1 mentions) Cytofix cytoperm kit, by BD (1 mentions) Cd11c fitc, by BioLegend (1 mentions) F4 80 apc cy7, by BioLegend (1 mentions) Myd88, by R&D Systems (1 mentions)

2 protocols using tlr3 pe

1

B Cell Phenotyping by Flow Cytometry

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At 24 h, B cells were washed with ice-cold PBS buffer and then stained with antibodies to CD19-APC, CD40-PE, CD86-PE, MHC II (Iab)-PE (BD Biosciences), or TLR3-PE (BioLegend, San Diego, CA, USA) for 30 min at 4°C. For intracellular TLR3 staining, cells were stained with antibody to CD19-APC and then fixed and permeabilised with a Cytofix/Cytoperm kit according to BD Biosciences. Permeabilised cells were incubated with PE-conjugated anti-TLR3 mAb. Data were collected using FACS Calibur (BD Biosciences) and analysed by FlowJo software.
Qian L., Chen W., Wang S., Liu Y., Jia X., Fu Y., Gong W, & Tian F. (2017). Immune complex negatively regulates toll-like receptor 3-triggered tumour necrosis factor α production in B cells. Central-European Journal of Immunology, 42(3), 223-230.
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2

Intracellular Staining of BAL Cells

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BAL cells were collected after LC. The cells were fixed, permeabilized, and subjected to intracellular staining. The following primary antibodies were used; MyD88 (R&D system, Minneapolis, MN) CD11c-FITC, TLR3-PE, and F4/80-APC-Cy7 (® BioLegend, San Diego, CA). Data were analyzed using Flow Jo software.
Suresh M.V., Thomas B., Machado-Aranda D., Dolgachev V.A., Ramakrishnan S.K., Talarico N., Cavassani K., Sherman M.A., Hemmila M.R., Kunkel S.L., Walter N.G., Hogaboam C.M, & Raghavendran K. (2016). Double-stranded RNA Interacts with Toll-like Receptor 3 in Driving the Acute Inflammatory Response Following Lung Contusion. Critical care medicine, 44(11), e1054-e1066.
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