Pe conjugated anti cd24

Manufactured by BioLegend

Sourced in United States

PE)-conjugated anti-CD24 is a fluorescently labeled monoclonal antibody that binds to the CD24 cell surface antigen. CD24 is expressed on various cell types, including hematopoietic cells, and can be used as a marker in flow cytometric analysis.

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Lab products found in correlation

Apc conjugated anti cd44, by BioLegend (1 mentions) Apc conjugated anti epcam, by BioLegend (1 mentions) Facsaria 2 cell sorter, by BD (1 mentions) Ic fixation buffer, by Thermo Fisher Scientific (1 mentions) Apc conjugated anti cd44, by Thermo Fisher Scientific (1 mentions) Facs buffer, by Thermo Fisher Scientific (1 mentions) Pe conjugated anti il 17a, by Thermo Fisher Scientific (1 mentions) Pe conjugated anti icos, by Thermo Fisher Scientific (1 mentions) Fitc conjugated anti cd3, by Thermo Fisher Scientific (1 mentions) Percp cy5.5 conjugated anti igd, by BioLegend (1 mentions) Pe cy7 conjugated anti cd19, by BioLegend (1 mentions)

3 protocols using pe conjugated anti cd24

Phenotyping of Cancer Stem Cells

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Cells were stained with the following antibodies from Biolegend (San Diego, CA): allophycocyanin (APC)-conjugated anti-CD44 (RRID:AB_312963), phycoerythrin (PE)-conjugated anti-CD24 (RRID:AB_314854) and APC-conjugated anti-EpCAM (RRID:AB_756081). FACS analysis of stained cells was performed using a FACSAria II cell sorter (Becton Dickinson, Franklin Lakes, NJ) as previously described (Lo et al., 2016 (link)).

Lo P.K., Yao Y., Lee J.S., Zhang Y., Huang W., Kane M.A, & Zhou Q. (2018). LIPG signaling promotes tumor initiation and metastasis of human basal-like triple-negative breast cancer. eLife, 7, e31334.

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Flow Cytometric Analysis of CD24/CD44 Expression

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The stably infected cells were collected and washed with PBS. Then, the cells were suspended in FACS buffer (00‐4222‐57, eBioscience) and stained with PE‐conjugated anti‐CD24 (311 105, BioLegend) and APC‐conjugated anti‐CD44 (338 805, eBioscience) at 4°C for 30 minutes. After staining, the cells were washed with PBS and resuspended in IC Fixation Buffer (00‐8222‐49, eBioscience) prior to FACS analysis.

Han L., Yan Y., Zhao L., Liu Y., Lv X., Zhang L., Zhao Y., Zhao H., He M, & Wei M. (2020). LncRNA HOTTIP facilitates the stemness of breast cancer via regulation of miR‐148a‐3p/WNT1 pathway. Journal of Cellular and Molecular Medicine, 24(11), 6242-6252.

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Multiparametric Immune Profiling Protocol

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Fluorescein isothiocyanate (FITC)-conjugated anti-CD38, Alexa 488-anti-CXCR5, Alexa F-647-anti-CXCR5, phycoerythrin (PE)-conjugated anti-IL-21, PE-Cy7-conjugated anti-CD4, allophycocyanin (APC)-conjugated anti-IL-6 and FITC-conjugated anti-IFN-gamma were purchased from BD Bioscience and BD Pharmingen™ (San Diego, CA, USA). FITC-conjugated anti-CD3, PE-conjugated anti-ICOS, PE-conjugated anti-IL-17a, peridinin chlorophyll protein (PerCP)-conjugated anti-CD4 and anti-CD8 and APC-conjugated anti-CD279 (PD-1) were purchased from eBioscience (San Diego, CA, USA); and APC-Cy7-conjugated anti-CD3 and anti-CD27, PE-conjugated anti-CD24, PE-Cy7-conjugated anti-CD19 and PerCP-Cy5.5-conjugated anti-IgD were purchased from BioLegend (San Diego, CA, USA). Peripheral blood mononuclear cells (PBMCs) were isolated and phenotypic analysis performed using optimal concentrations of mAbs according to standard protocols (25 (link), 26 (link)). Aliquots of cells were also utilized for flow cytometry and FlowJo software analysis (Tristar Inc, San Carlos, CA, USA). At least 50,000 events per sample were analyzed.

Wang L., Sun Y., Zhang Z., Jia Y., Zou Z., Ding J., Li Y., Xu X., Jin L., Yang T., Li Z., Sun Y., Zhang J.Y., Lv S., Chen L., Li B., Gershwin M.E, & Wang F.S. (2015). CXCR5+ CD4+ T Follicular Helper Cells Participate in the Pathogenesis of Primary Biliary Cirrhosis. Hepatology (Baltimore, Md.), 61(2), 627-638.

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