Nilotinib and anti-BAFF-R B-1239 antibodies (unconjugated and Alexa-647 labeled) were from Novartis. These antibodies were selected from the Human Combinatorial Antibody Library (HuCAL GOLD{r}), using phage display technology (8 (link)). The B-1239 mAb was produced in a fucosyl-transferase deficient CHO cell line (BioWa Potelligent Technology, BioWa Inc., Princeton, NJ, USA) (20 (link)), with a humanized sequence to optimalize ADCC activity. B-1239 was selected based on its property of blocking the BAFF/BAFF-R interaction and signaling via BAFF. Anti-BAFF-R antibody clone 11c1, anti-human CD19 and CD10 antibodies were from BD Biosciences (San Jose, CA, USA). FITC-anti-human IgG was from Sigma Aldrich (CA, USA). Recombinant huBAFF and the function-blocking anti-BAFF-R antibodies were purchased from R&D Systems (MN, USA). Bcr N-20, BAFF-R and Gapdh antibodies for Western blotting were from Santa Cruz, eBiosciences and Millipore, respectively. Cell Mask Orange and Deep Red were from Life Technologies (Eugene, OR).
Anti baff r antibody clone 11c1
Manufactured by BD
Sourced in United States
The Anti-BAFF-R antibody clone 11c1 is a laboratory reagent used for the detection and analysis of the BAFF-R (B-cell activating factor receptor) protein. This antibody can be used in various immunoassay techniques, such as flow cytometry, to identify and quantify cells expressing the BAFF-R protein.
Automatically generated - may contain errors
Lab products found in correlation
Cellmask orange, by Thermo Fisher Scientific (1 mentions)
Anti human cd19, by BD (1 mentions)
Anti human igg fitc, by Merck Group (1 mentions)
Nilotinib, by Novartis (1 mentions)
Nfkb p100 p52, by Cell Signaling Technology (1 mentions)
Apc anti human cd19 antibody, by BioLegend (1 mentions)
Gapdh, by R&D Systems (1 mentions)
Apc annexin 5 apoptosis detection kit with pi, by BioLegend (1 mentions)
Total akt antibody, by Cell Signaling Technology (1 mentions)
Lamin a c, by Santa Cruz Biotechnology (1 mentions)
α tubulin, by Santa Cruz Biotechnology (1 mentions)
Cleaved caspase 3, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti baff r antibody clone 11c1
1
Blocking BAFF-R Signaling in B Cells
2
Investigating BAFF-R Signaling Pathway
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Recombinant human BAFF (rhBAFF), APC anti-human CD19 antibody, APC Annexin V apoptosis detection kit with PI (BioLegend), Anti-BAFF-R VAY-736 (Novartis), Anti-BAFF- R antibody clone 11c1 (Pharmingen), cytarabine, Fluorescein isothiocyanate (FITC) anti-human immunoglobulin G (IgG) (Sigma Aldrich), neutralizing anti-human BAFF R (R&D Systems), GAPDH, Lamin A/C, α-tubulin, cleaved caspase 3, total-ERK antibodies (Santa Cruz Biotechnology), NF-kB p100/p52, phospho-AKT(Ser473), phospho- ERK1/2(Thr202/Tyr204), total-AKT antibodies (Cell Signaling Technology) were used for the study.
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