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ABS983 is a laboratory instrument designed for the detection and quantification of biomolecules. It utilizes advanced spectroscopic techniques to analyze a wide range of samples, including proteins, nucleic acids, and small molecules. The core function of ABS983 is to provide accurate and reliable data for researchers and scientists working in the life sciences and biotechnology fields.

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Lab products found in correlation

Ab82411, by Thermo Fisher Scientific (3 mentions) Ab16045, by Abcam (3 mentions) Mtp11 s, by Merck Group (3 mentions) Sc 365365, by Santa Cruz Biotechnology (3 mentions) Sc 65882, by Abcam (3 mentions) Criterion tgx precast protein gel, by Bio-Rad (3 mentions) Ma5 27915, by Abcam (2 mentions) Ecl reagent, by PerkinElmer (2 mentions) Corresponding secondary antibody conjugated to hrp, by GE Healthcare (2 mentions)

3 protocols using abs983

1

Western Blot Analysis of Iron-Regulatory Proteins

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples were loaded onto a 4–20% Criterion TGX Precast Protein Gel (Bio-Rad) [7 (link)]. Protein was transferred onto a nitrocellulose membrane and probed for Fpn (Alpha Diagnostics, MTP11-S, 1:1000), DMT1 (Millipore, ABS983, 1:1000), Heph (Santa Cruz, SC-365365, 1:1000), TfR (Santa Cruz, sc-65882, 1:250), Tf (Abcam, ab82411, 1:1000), HA tag (Invitrogen, MA5-27915, 1:1000), ubiquitin (Protein Tech, 10201–2-AP, 1:1000) or cyclophilin B (Abcam, ab16045, 1:1000) as a loading control. Corresponding secondary antibody conjugated to HRP was used (1:5000, GE Amersham) and bands were visualized using ECL reagents (Perkin-Elmer) on an Amersham Imager 600 (GE Amersham). Cellular lysate samples were normalized to cyclophilin B protein as a loading control, and then subsequently normalized to an untreated control sample within each experiment. Membrane protein samples were stained with Ponceau S and normalized to total protein as a loading control.
Baringer S.L., Palsa K., Spiegelman V.S., Simpson I.A, & Connor J.R. (2023). Apo- and holo-transferrin differentially interact with hephaestin and ferroportin in a novel mechanism of cellular iron release regulation. Journal of Biomedical Science, 30, 36.
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2

Western Blot Analysis of Iron Transporters

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples were loaded onto a 4–20% Criterion TGX Precast Protein Gel (Bio-Rad)7 (link). Protein was transferred onto a nitrocellulose membrane and probed for Fpn (Alpha Diagnostics, MTP11-S, 1:1000), DMT1 (Millipore, ABS983, 1:1000), Heph (Santa Cruz, SC-365365, 1:1000), TfR (Santa Cruz, sc-65882, 1:250), Tf (Abcam, ab82411, 1:1000), HA tag (Invitrogen, MA5-27915, 1:1000), or cyclophilin B (Abcam, ab16045, 1:1000) as a loading control. Corresponding secondary antibody conjugated to HRP was used (1:5000, GE Amersham) and bands were visualized using ECL reagents (Perkin-Elmer) on an Amersham Imager 600 (GE Amersham). Cellular lysate samples were normalized to cyclophilin B protein as a loading control, and then subsequently normalized to an untreated control sample within each experiment. Membrane protein samples were stained with Ponceau S and normalized to total protein as a loading control.
Baringer S., Palsa K., Simpson I.A, & Connor J.R. (2023). Apo- and holo- transferrin differentially interact with ferroportin and hephaestin to regulate iron release at the blood-brain barrier. Research Square.
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3

Western Blot Analysis of Iron Transporters

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples were loaded onto a 4–20% Criterion TGX Precast Protein Gel (Bio-Rad)7 (link). Protein was transferred onto a nitrocellulose membrane and probed for Fpn (Alpha Diagnostics, MTP11-S, 1:1000), DMT1 (Millipore, ABS983, 1:1000), Heph (Santa Cruz, SC-365365, 1:1000), TfR (Santa Cruz, sc-65882, 1:250), Tf (Abcam, ab82411, 1:1000), HA tag (Invitrogen, MA5-27915, 1:1000), or cyclophilin B (Abcam, ab16045, 1:1000) as a loading control. Corresponding secondary antibody conjugated to HRP was used (1:5000, GE Amersham) and bands were visualized using ECL reagents (Perkin-Elmer) on an Amersham Imager 600 (GE Amersham). Cellular lysate samples were normalized to cyclophilin B protein as a loading control, and then subsequently normalized to an untreated control sample within each experiment. Membrane protein samples were stained with Ponceau S and normalized to total protein as a loading control.
Baringer S.L., Palsa K., Simpson I.A, & Connor J.R. (2023). Apo- and holo- transferrin differentially interact with ferroportin and hephaestin to regulate iron release at the blood-brain barrier. bioRxiv.
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