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Rabbit igg isotype control

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Rabbit IgG isotype control is a laboratory reagent used as a negative control in immunoassays and other applications that involve the use of primary antibodies. It is a purified immunoglobulin G (IgG) derived from rabbit serum that lacks reactivity to specific target antigens, providing a baseline for assessing non-specific binding of primary antibodies.

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Lab products found in correlation

Lsm800 confocal microscope, by Zeiss (2 mentions) Horseradish peroxidase conjugated anti rabbit igg, by Santa Cruz Biotechnology (1 mentions) Phusion 2x hf master mix, by New England Biolabs (1 mentions) H3k27ac antibody, by Abcam (1 mentions) Minelute pcr purification kit, by Qiagen (1 mentions) Minelute gel extraction kit, by Qiagen (1 mentions) Glutamate, by Merck Group (1 mentions) Rapamycin, by Merck Group (1 mentions) Cellmask green plasma membrane stain, by Thermo Fisher Scientific (1 mentions) Trifluoperazine tfp, by Merck Group (1 mentions) Hoechst 33342 trihydrochloride, by Thermo Fisher Scientific (1 mentions) Lipopolysaccharides lps from escherichia coli o111 b4, by Merck Group (1 mentions) Mk 801, by Merck Group (1 mentions) Bapta am, by Merck Group (1 mentions) Paraformaldehyde (pfa), by Biosesang (1 mentions) Mouse igg1 isotype control, by Thermo Fisher Scientific (1 mentions) Fitc conjugated mouse igg1 isotype control, by Thermo Fisher Scientific (1 mentions) Fitc conjugated annexin 5, by Beckman Coulter (1 mentions) Countbright absolute counting beads, by Thermo Fisher Scientific (1 mentions) Tissue tek embedding medium, by Sakura Finetek (1 mentions)

Close spelling variants of this product

IgG isotype control Rabbit IgG isotype Control rabbit IgG Isotype control Isotype IgG Control IgG IgG rabbit

11 protocols using rabbit igg isotype control

1

Immunohistochemical Analysis of USP18 in Muscle

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Eight-μm-thick unfixed cryostat muscle sections were collected from diagnostic muscle biopsies. Anti-human ubiquitin-specific peptidase 18 (USP18) polyclonal antibodies (Abcam, Cambridge, UK) were used as primary antibodies for detecting USP18 protein at a working concentration of 5 μg/ml. Horseradish peroxidase-conjugated anti-rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, USA) was used as a secondary antibody. Rabbit IgG isotype control (Abcam, Cambridge, UK) was used as a negative control for the primary antibody. The immunohistochemistry staining was performed as previously described44 (link).
Peng Q.L., Zhang Y.M., Yang H.B., Shu X.M., Lu X, & Wang G.C. (2016). Transcriptomic profiling of long non-coding RNAs in dermatomyositis by microarray analysis. Scientific Reports, 6, 32818.
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2

ChIP-seq Library Preparation Reagents

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MinElute PCR Purification Kit (Qiagen catalog # 28004).

MinElute Gel Extraction Kit (Qiagen catalog # 28604).

Phusion 2X HF Master Mix (New England Biolabs catalog # M0531S).

H3K4me3 antibody (Millipore Sigma catalog # 07-473).

H3K27ac antibody (Abcam catalog # ab4729).

RNA Pol II antibody (Millipore Sigma catalog # 17-620).

Rabbit IgG isotype control (Abcam catalog # ab37415).

Carter B., Ku W.L., Pelt J, & Zhao K. (2021). Concurrent mapping of multiple epigenetic marks and co-occupancy using ACT2-seq. Cell & Bioscience, 11, 198.
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3

Neurochemical Modulation of Cell Signaling

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All chemical reagents including hexadecyl trimethyl ammonium bromide (CTAB), tetraethyl orthosilicate, 3-mercaptopropyl-triethoxysilane (MPTES), lipopolysaccharides (LPS) from Escherichia coli O111:B4, MK-801, glutamate, NMDA, rapamycin, BAPTA-AM, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), and trifluoperazine (TFP) were purchased from Sigma Aldrich Chemical Co. (St. Louis, MO, USA). Rabbit IgG isotype control was obtained from Abcam (Cambridge, United Kingdom). NMDAR1 polyclonal antibody was obtained from Invitrogen of Thermo Fisher Scientific Co. (Waltham, MA, USA). CellMask™ green plasma membrane stain and Hoechst 33342 (Trihydrochloride) were purchased from Thermo Fisher Scientific. Phosphate buffered saline (PBS, pH7.4) and 4% paraformaldehyde (PFA) were supplied by Biosesang (Seoungnam, South Korea). FSD Fluor™ 647 dye was purchased from BioActs Co. (Incheon, South Korea).
Jeon H.J., Byun J.K., Lee S.B., Son K.H., Lim J.Y., Lee D.S., Kim K.S., Park J.W., Shin G.R., Kim Y.J., Jin J., Kim D., Kim D.H., Yu J.H., Choi Y.K., Park K.G, & Jeon Y.H. (2023). N-methyl-d-aspartate receptors induce M1 polarization of macrophages: Feasibility of targeted imaging in inflammatory response in vivo. Cell & Bioscience, 13, 69.
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4

Immunoprecipitation of CPSF6 Protein

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Dynabeads were precoated with rabbit anti-human CPSF6 (number 175237; Abcam) or rabbit IgG isotype control (number 172730; Abcam) at 4°C for 4 h (ThermoFisher). Cell lysates were added to antibody-precoated beads and kept rolling at 4°C overnight. Proteins were then eluted in NETN+/+-containing 1× Laemmli buffer (2% SDS, 0.1% bromophenol blue, 7.8% glycerol, 10 mM Tris, pH 6.8, 1.5% dithiothreitol) by heating at 95°C for 10 min. The proteins were further analyzed by Western blotting.
Zheng Y., Schubert H.L., Singh P.K., Martins L.J., Engelman A.N., D’Orso I., Hill C.P, & Planelles V. (2021). Cleavage and Polyadenylation Specificity Factor 6 Is Required for Efficient HIV-1 Latency Reversal. mBio, 12(3), e01098-21.
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5

Antibody Panel for Alzheimer's Biomarkers

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The following antibodies were employed: rabbit anti-Aβ1-42 monoclonal antibody (clone H31L21, Invitrogen, Carlsbad, CA), which recognizes human and mouse Aβ1-42; cross reactivity to Aβ1-40 is not observed in sandwich ELISA according to the manufacturer’s instructions; mouse anti-human Aβ monoclonal antibody (clone 6E10, Covance, Princeton, NJ), mouse anti-human apoE monoclonal antibody (clone 1H4, Abcam, Cambridge, MA); mouse anti-human apoJ monoclonal antibody (clone 3R3/2, Lifespan Biosciences, Seattle, WA); rabbit anti-mouse apoJ polyclonal antibody (Lifespan Biosciences); fluorescein isothiocyanate (FITC)-conjugated mouse anti-human apoAI monoclonal antibody (clone APO-1-1, Fitzgerald Industries, Acton, MA); rabbit anti-mouse apoAI polyclonal antibody (Lifespan Biosciences); FITC-conjugated annexin V (Beckman Coulter, Pasadena, CA); and mouse anti-human tau monoclonal antibody (clone TAU-5, Abcam). Isotype controls were: FITC-conjugated mouse IgG1 isotype control (eBioscience, San Diego, CA); mouse IgG1 isotype control (eBioscience), rabbit IgG isotype control (Abcam). Other reagents included Calibration BeadMix (Apogee Flow Systems, Hemel Hempstead, UK), annexin V binding buffer 10× (Beckman Coulter), CountBright absolute counting beads (Invitrogen), Zenon Alexa Fluor 488 mouse IgG1 and phycoerythrin (PE) rabbit IgG labeling kits (Invitrogen).
Yang Y., Keene C.D., Peskind E.R., Galasko D.R., Hu S.C., Cudaback E., Wilson A.M., Li G., Yu C.E., Montine K.S., Zhang J., Baird G.S., Hyman B.T, & Montine T.J. (2015). Cerebrospinal Fluid Particles in Alzheimer Disease and Parkinson Disease. Journal of neuropathology and experimental neurology, 74(7), 672-687.
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6

Immunohistochemical Staining of Eyelid

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Eyelids were collected and immediately fixed in 4% buffered paraformaldehyde overnight and then embedded in Tissue-Tek embedding medium (Sakura Finetek USA, Inc., Torrance, CA, USA) for cryosectioning. Sections (10 μm) were cut using a Leica CM 1950 (Leica, Buffalo Grove, IL, USA) cryostat and collected on Fisherbrand SuperfrostPlus Gold microscope slides (Thermo Fisher Scientific). Upon use, sections were incubated for 30 minutes at 60°C, and excess tissue embedding medium was removed with PBS. Unspecific protein binding sites were blocked with 10% fetal bovine serum (FBS) prepared in PBS containing 0.01 M saponin. Sections were then incubated with the primary antibodies anti-Krt14 (PRB-155P; Covance, Princeton, NJ, USA) and HA binding protein (Millipore). Sections were washed and incubated with NeutrAvidin Alexa Fluor 555 conjugate and anti-rabbit produced in donkey conjugated with Alexa 488 for 1 hour at room temperature. A secondary control was carried out with rabbit IgG isotype control (Abcam, Cambridge MA, USA) in place of the primary antibody and did not yield any staining (results not shown). Slides were mounted in Fluoromount-G and imaged under an LSM 800 confocal microscope (Zeiss).
Sun M., Puri S., Parfitt G.J., Mutoji N, & Coulson-Thomas V.J. (2018). Hyaluronan Regulates Eyelid and Meibomian Gland Morphogenesis. Investigative Ophthalmology & Visual Science, 59(8), 3713-3727.
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7

FBXW7 Expression Analysis in CRC Cells

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CRC cells were dissociated with trypsin/EDTA, washed with PBS, and fixed with 4% paraformaldehyde. The cells were permeabilized using ice-cold methanol for 30 min. After washing, nonspecific antibody binding sites were blocked with 5% normal goat serum/PBS for 30 min. Cells were then incubated with rabbit anti-human FBXW7 monoclonal antibody (1:200 dilution, clone SP237, Abcam, Cambridge, UK) or rabbit IgG isotype control (Abcam) for 30 min at 4 °C, followed by APC conjugated goat anti-rabbit IgG secondary antibody (Abcam) for 30 min at room temperature. These samples were analyzed using a FACS Aria II cell sorter.
Honma S., Hisamori S., Nishiuchi A., Itatani Y., Obama K., Shimono Y, & Sakai Y. (2019). F-Box/WD Repeat Domain-Containing 7 Induces Chemotherapy Resistance in Colorectal Cancer Stem Cells. Cancers, 11(5), 635.
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8

Immunostaining of IL-36R in Macrophages

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Isolated macrophages were seeded onto coverslips overnight. Cells were washed in PBS and fixed in 4% formaldehyde for 20 min. Cells were then blocked for 1 h in 5% BSA in PBS. Cells were incubated overnight with rabbit anti-human IL-36R 1:500 (Novus Biologics, Littleton, CO, USA) or rabbit IgG isotype control (1:500; Abcam, Cambridge, UK). Cells were then washed with PBS and incubated with donkey anti-rabbit Alexa 594 conjugated and mouse anti-human CD14 FITC conjugated or mouse IgG isotype control (both 1:100; both ImmunoTools, Friesoythe, Germany). Cells were visualised using the EVOS XL microscope (Thermo Fisher Scientific).
Bridgewood C., Fearnley G.W., Berekmeri A., Laws P., Macleod T., Ponnambalam S., Stacey M., Graham A, & Wittmann M. (2018). IL-36γ Is a Strong Inducer of IL-23 in Psoriatic Cells and Activates Angiogenesis. Frontiers in Immunology, 9, 200.
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9

Western Blot Immunoblotting Reagents

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Sodium dodecylsulfate (SDS), epigallocatechin-3-gallate (EGCG) and bovine serum albumin (BSA) were purchased from Sigma-Aldrich Canada (Oakville, ON, Canada). Electrophoresis reagents were purchased from Bio-Rad (Mississauga, ON, Canada). The enhanced chemiluminescence (ECL) reagents were from Amersham Pharmacia Biotech (Baie d’Urfé, QC, Canada). Micro bicinchoninic acid protein assay reagents were purchased from Pierce (Rockford, IL, USA). The polyclonal antibodies against Snail, Slug, phosphorylated and total NF-κβ (p105), Smad2, and STAT3 were obtained from Cell Signaling Technology Inc. (Danvers, MA, USA). Monoclonal antibody (mAb) against human IL-6 was purchased from (New England Biolabs Ltd., Whitby, ON, Canada), rabbit IgG isotype control was obtained from Abcam (Cat ab172730, clone EPR25A); and a mAb against glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was from Advanced Immunochemical Inc. (Long Beach, CA, USA). Horseradish peroxidase-conjugated donkey anti-rabbit and anti-mouse IgG secondary antibodies were obtained from Jackson ImmunoResearch Laboratories (West Grove, PA, USA). Protein A sepharose beads were obtained from GE Healthcare (Uppsala, Sweden). Gelatin was obtained from Sigma Aldrich (Oakville, ON, Canada).
Gonzalez Suarez N., Fernandez-Marrero Y., Torabidastgerdooei S, & Annabi B. (2022). EGCG Prevents the Onset of an Inflammatory and Cancer-Associated Adipocyte-like Phenotype in Adipose-Derived Mesenchymal Stem/Stromal Cells in Response to the Triple-Negative Breast Cancer Secretome. Nutrients, 14(5), 1099.
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10

Immunofluorescence Staining of HUVEC and HDLEC

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HUVEC and HDLEC were seeded onto gelatin coated coverslips overnight. Cells were washed in TBS and fixed in 4% formaldehyde for 20 mins. Cells were then blocked for 1 hour in 5% BSA in TBS. Cells were incubated overnight with mouse anti-human CD31
(1:1000) (Dako, Glostrup, Denmark) and rabbit anti-human IL-36R (1:500) (Novus Biologicals, Littleton, USA) or Rabbit IgG isotype control (1:500, Abcam, Cambridge, UK).
Cells were washed with TBST and secondary donkey anti-rabbit Alexa 594 conjugated, and donkey anti-mouse Alexa 488 conjugated were added (both 1:1000, both Invitrogen, Carlsbad, USA).
Bridgewood C., Stacey M., Alase A., Lagos D., Graham A, & Wittmann M. (2017). IL-36γ has proinflammatory effects on human endothelial cells. Experimental dermatology, 26(5).
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