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Opto engine

Manufactured by Thorlabs
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The Opto Engine is a compact, high-performance optical engine designed for a variety of applications. It features a semiconductor laser source, collimating optics, and a modular housing for easy integration into custom setups.

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Lab products found in correlation

Zirconia sleeve, by Doric (2 mentions) Waveform generator, by Arduino (2 mentions) Laserglow, by Thorlabs (2 mentions) Pm20a, by Thorlabs (2 mentions) Fiber optic rotary joint, by Doric (2 mentions) Pclamp 10, by Molecular Devices (1 mentions)

3 protocols using opto engine

1

Optogenetic Stimulation of Neuronal Targets

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Mice were allowed to adapt to the fiber patch cord for at least three days prior to experiments and typically not handled on the day of the experiment. Fiber optic cables (200 m diameter; NA: 0.22, 1 m long; Doric Lenses; or, 0.5m long, ThorLabs) were connected to the implanted fiber optic cannulas with zirconia sleeves (Doric Lenses) and coupled to lasers via a fiber optic rotary joint (Doric Lenses). We adjusted the light power of the laser (473 nm; Laserglow or Opto Engine) such that the light power (measured with a fiber optic power meter; PM20A; ThorLabs) at the end of the fiber optic cable was ~10 mW. Using an online light transmission calculator for brain tissue (http://web.stanford.edu/group/dlab/cgi-bin/graph/chart.php), we estimated the light power at the DMH or RPa between 3 and 6 mW/mm2. This is an upper limit due to possible light loss between the fiber optic cable and the implanted optic fiber. Light pulses were controlled by a waveform generator (Arduino) programmed to deliver light pulses. In most experiments (unless otherwise indicated), stimulation was on for 1 s, followed by 3 s off, pulses were 10 ms delivered at 20 Hz. After the completion of experiments, fiber placement and ChR2 expression were assessed. Animals without ChR2 expression or incorrect placement of optic fibers were excluded from analysis.
Piñol R.A., Zahler S.H., Li C., Saha A., Tan B.K., Škop V., Gavrilova O., Xiao C., Krashes M.J, & Reitman M.L. (2018). Brs3 neurons in the mouse dorsomedial hypothalamus regulate body temperature, energy expenditure and heart rate, but not food intake. Nature neuroscience, 21(11), 1530-1540.
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2

Optogenetic Stimulation of Neuronal Targets

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Mice were allowed to adapt to the fiber patch cord for at least three days prior to experiments and typically not handled on the day of the experiment. Fiber optic cables (200 m diameter; NA: 0.22, 1 m long; Doric Lenses; or, 0.5m long, ThorLabs) were connected to the implanted fiber optic cannulas with zirconia sleeves (Doric Lenses) and coupled to lasers via a fiber optic rotary joint (Doric Lenses). We adjusted the light power of the laser (473 nm; Laserglow or Opto Engine) such that the light power (measured with a fiber optic power meter; PM20A; ThorLabs) at the end of the fiber optic cable was ~10 mW. Using an online light transmission calculator for brain tissue (http://web.stanford.edu/group/dlab/cgi-bin/graph/chart.php), we estimated the light power at the DMH or RPa between 3 and 6 mW/mm2. This is an upper limit due to possible light loss between the fiber optic cable and the implanted optic fiber. Light pulses were controlled by a waveform generator (Arduino) programmed to deliver light pulses. In most experiments (unless otherwise indicated), stimulation was on for 1 s, followed by 3 s off, pulses were 10 ms delivered at 20 Hz. After the completion of experiments, fiber placement and ChR2 expression were assessed. Animals without ChR2 expression or incorrect placement of optic fibers were excluded from analysis.
Piñol R.A., Zahler S.H., Li C., Saha A., Tan B.K., Škop V., Gavrilova O., Xiao C., Krashes M.J, & Reitman M.L. (2018). Brs3 neurons in the mouse dorsomedial hypothalamus regulate body temperature, energy expenditure and heart rate, but not food intake. Nature neuroscience, 21(11), 1530-1540.
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3

Optogenetic Dissection of Synaptic Connectivity

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For CRACM, photostimulation-evoked responses were recorded using a Cs+ based, low Cl− internal solution consisting of (in mM): 135 CsMeSO3, 10 HEPES, 1 EGTA, 4 MgCl2, 4 Na2-ATP, 0.4 Na2-GTP, 10 Na2-phosphocreatine (pH 7.3 adjusted with CsOH; 295 mOsm · kg−1; ECl = −70 mV). Light-evoked EPSCs and IPSCs were recorded in whole-cell voltage clamp mode with the membrane potential clamped at Vh = −70 mV and 0 mV, respectively. CNQX and D-AP5 or bicuculline were included to isolate glutamatergic/GABAergic currents as needed. A laser or LED light source (473 nm; Opto Engine; Thorlabs) was used to evoke ChR2-mediated responses. The light output was controlled by a programmable pulse stimulator, Master-8 (A.M.P.I.) and pClamp 10.2 software (Axon Instruments). Photostimulation-evoked EPSCs/IPSCs detection protocol consisted of 4X 5msec blue light laser pulses administered 1s apart during and 8 second sweep, repeated for a total of 30 sweeps. Evoked EPSCs with short latency (≤6 ms) upon light stimulation were considered as light-driven. As discussed by others (Petreanu et al., 2007), such currents are most likely monosynaptic. TTX 5uM was applied in several cells to demonstrate definitive monosynaptic connectivity.
Li M.M., Madara J.C., Steger J.S., Krashes M.J., Balthasar N., Campbell J.N., Resch J.M., Conley N.J., Garfield A.S, & Lowell B.B. (2019). The paraventricular hypothalamus regulates satiety and prevents obesity via two genetically distinct circuits. Neuron, 102(3), 653-667.e6.
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